Diphenhydramine colloidal gold test strip and kit thereof

A technology of diphenhydramine and test strips, which is applied in the field of diphenhydramine colloidal gold detection test strips and its kit, can solve the problems of mass spectrometry instrument damage, low detection sensitivity, long processing and detection time, etc., and achieve Reliable detection results, high detection sensitivity and low detection cost

Pending Publication Date: 2021-03-09
广东省药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing laboratory detection technology has technical defects such as low sensitivity, long time consumption and insufficient mobility
Common laboratory methods for detecting the illegal addition of diphenhydramine are liquid chromatography or liquid chromatography-mass chromatography. Both methods have long sample pretreatment and detection time, and are not convenient for on-site supervision and detection.
At the same time, liquid chromatography has the disadvantage of low detection sensitivity. Although liquid chromatography-mass spectrometry detection method has high sensitivity, it also has high requirements for sample pretreatment methods, otherwise it will easily cause mass spectrometry contamination to affect the detection results or directly cause damage to the mass spectrometer.

Method used

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  • Diphenhydramine colloidal gold test strip and kit thereof
  • Diphenhydramine colloidal gold test strip and kit thereof
  • Diphenhydramine colloidal gold test strip and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of embodiment 1 diphenhydramine colloidal gold detection test strip

[0047] 1. Preparation of colloidal gold

[0048] Take 2 mL of 1% chloroauric acid solution, add 98 mL of ultrapure water to form a chloroauric acid solution with a final concentration of 0.02%, and after heating and boiling, take 1.2 mL of 2% trisodium citrate and quickly add it to the boiling chloroauric acid solution at one time. Continue to heat until the solution turns from light yellow to blue-black and finally to bright red. After the color is stable, continue to heat for 30 minutes, cool at room temperature, and replenish the lost water to the original volume.

[0049] 2. Preparation of colloidal gold-labeled monoclonal antibody

[0050] For the preparation of colloidal gold-labeled monoclonal antibody, adjust the pH value of the colloidal gold solution to 8.0, stir evenly with a constant speed stirrer for 10 minutes, add diphenhydramine monoclonal antibody dropwise after 10 mi...

Embodiment 2

[0053] The preparation of embodiment 2 diphenhydramine colloidal gold detection kit

[0054] The test strip in Example 1 is further assembled into a kit, wherein the kit also includes a diluent, a desiccant and a dropper. Wherein the diluent is PBST buffer.

Embodiment 3

[0055] Embodiment 3 detects diphenhydramine colloidal gold detection test paper false positive rate

[0056] The false positive rate is one of the most important indicators for examining the detection accuracy of colloidal gold test strips. The lower the false positive rate is, the more reliable the test results of colloidal gold test strips are. In this embodiment, the false positive rate of diphenhydramine colloidal gold test strips is detected with herbal tea as a sample.

[0057] Through the detection of 30 batches of negative herbal tea samples, the accuracy of the diphenhydramine colloidal gold test strip detection results prepared in Example 1 was investigated.

[0058] Herbal tea samples are purchased from stores in the market, including special cold tea, wind-cold and phlegm-relieving cough tea, herbal tea king, throat pain relief tea, fire-relieving and detoxifying tea, cold tea, sore throat tea, cough-relieving tea, heat-clearing and detoxifying tea, cold cough tea ...

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Abstract

The invention discloses a diphenhydramine colloidal gold test strip which comprises a bottom plate, and a sample pad, a conjugate pad, a nitrocellulose membrane, absorbent paper and a clamping shell which are sequentially laid on the bottom plate, wherein a detection line and a quality control line are marked on the nitrocellulose membrane, a colloidal gold labeled antibody of diphenhydramine is arranged on the combination pad, and the colloidal gold labeled antibody is formed by coupling colloidal gold and a diphenhydramine monoclonal antibody. The test strip is rapid, accurate, flexible andmobile, is suitable for on-site supervision and detection of diphenhydramine, and can rapidly and accurately detect whether a sample contains diphenhydramine or not in a short time so as to determinewhether diphenhydramine is illegally added or not. The test strip is suitable for quality control detection of production enterprises such as herbal tea and beverages/make-up liquids/Chinese patent medicines or supervision and inspection of the consumer goods by government detection institutions. Compared with the prior art, the method has the characteristics of high detection sensitivity, high detection accuracy, convenience and quickness in operation, low detection cost and the like.

Description

technical field [0001] The invention belongs to the technical field of colloidal gold detection, and in particular relates to a diphenhydramine colloidal gold detection test strip and a kit thereof. Background technique [0002] Diphenhydramine is an alcohol-based antihistamine, which mainly has anti-allergic, anti-emetic, antitussive, and sedative effects. Therefore, diphenhydramine may be added to functional cosmetics for acne, anti-allergic, mosquito bites, etc., and added to functional drinks or Chinese patent medicines for colds to relieve cough and asthma. The effective oral dose for children is 12.5 mg / time for prevention or treatment of diseases, and 25 mg / time for adults. As the first generation of antihistamines, diphenhydramine itself also produces many side effects when treating diseases, such as central nervous system depression, nausea, loss of appetite, chest tightness, anaphylactic shock, convulsions, etc. Consumers contact (oral or skin application) diphen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/543
CPCG01N33/9493G01N33/577G01N33/558G01N33/54346
Inventor 罗卓雅庄玥汪瑾彦杨鹏博范彬
Owner 广东省药品检验所
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