Diphenhydramine colloidal gold test strip and kit thereof

Abstract

The invention discloses a diphenhydramine colloidal gold test strip which comprises a bottom plate, and a sample pad, a conjugate pad, a nitrocellulose membrane, absorbent paper and a clamping shell which are sequentially laid on the bottom plate, wherein a detection line and a quality control line are marked on the nitrocellulose membrane, a colloidal gold labeled antibody of diphenhydramine is arranged on the combination pad, and the colloidal gold labeled antibody is formed by coupling colloidal gold and a diphenhydramine monoclonal antibody. The test strip is rapid, accurate, flexible andmobile, is suitable for on-site supervision and detection of diphenhydramine, and can rapidly and accurately detect whether a sample contains diphenhydramine or not in a short time so as to determinewhether diphenhydramine is illegally added or not. The test strip is suitable for quality control detection of production enterprises such as herbal tea and beverages / make-up liquids / Chinese patent medicines or supervision and inspection of the consumer goods by government detection institutions. Compared with the prior art, the method has the characteristics of high detection sensitivity, high detection accuracy, convenience and quickness in operation, low detection cost and the like.

Description

technical field [0001] The invention belongs to the technical field of colloidal gold detection, and in particular relates to a diphenhydramine colloidal gold detection test strip and a kit thereof. Background technique [0002] Diphenhydramine is an alcohol-based antihistamine, which mainly has anti-allergic, anti-emetic, antitussive, and sedative effects. Therefore, diphenhydramine may be added to functional cosmetics for acne, anti-allergic, mosquito bites, etc., and added to functional drinks or Chinese patent medicines for colds to relieve cough and asthma. The effective oral dose for children is 12.5 mg / time for prevention or treatment of diseases, and 25 mg / time for adults. As the first generation of antihistamines, diphenhydramine itself also produces many side effects when treating diseases, such as central nervous system depression, nausea, loss of appetite, chest tightness, anaphylactic shock, convulsions, etc. Consumers contact (oral or skin application) diphen...

AI Technical Summary

Problems solved by technology

[0003] The existing laboratory detection technology has technical defects such as low sensitivity, long time consumption and insufficient mobility

Common laboratory methods for detecting the illegal addition of diphenhydramine are liquid chromatography or liquid chromatography-mass chromatography. Both methods have long sample pretreatment and detection time, and are not convenient for on-site supervision and detection.

At the same time, liquid chromatography has the disadvantage of low detection sensitivity. Although liquid chromatography-mass spectrometry detection method has high sensitivity, it also has high requirements for sample pretreatment methods, otherwise it will easily cause mass spectrometry contamination to affect the detection results or directly cause damage to the mass spectrometer.

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