Triple immunofluorescence quantitative detection kit for cardiac troponin I, brain natriuretic peptide and D-dimer chest pain
A technique for cardiac troponin and brain natriuretic peptide, which is applied in biological testing, measuring devices, material testing products, etc., can solve problems such as lack of detection kits, and achieve the effects of improving sensitivity, uniform flow rate, and uniform detection line
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Embodiment 1
[0027] Example 1 Kit for joint quantitative detection of cardiac troponin I, brain natriuretic peptide and D-dimer
[0028] 1. Composition and preparation method of the kit
[0029] A kit for the joint quantitative detection of cardiac troponin I, brain natriuretic peptide and D-dimer, including: a test card, a U disk with a standard curve and a sample diluent.
[0030] (1) Structure of test card
[0031] combined figure 1 with figure 2 , illustrating the structure of the test card.
[0032] The test card includes a card shell and a test strip inside the card shell.
[0033] The test strip includes a substrate 6 and a sample pad 7 , a CP pad 8 , an NC film 9 and an absorbent pad 10 pasted on the substrate in sequence. Adjacent ends of the sample pad, CP pad, NC membrane, and absorbent pad overlap each other.
[0034] The cartridge includes an upper cartridge 1 and a lower cartridge 2 that are engaged with each other. The inner surface of the lower cartridge 2 is provide...
Embodiment 2
[0071] Embodiment 2 The effect contrast of test strip of the present invention and other test strips
[0072] Control test strips 1-4 were prepared respectively.
[0073] The NC film of the control test strip 1 is provided with T2 detection lines and quality control lines at equal intervals. The T2 detection line is coated with cTnI monoclonal antibody (purchased from Huakui Jinpei, product number A8H12), and the coating concentration is 1 mg / mL. The quality control line was coated with rabbit anti-chicken IgY monoclonal antibody (purchased from Wuhan Yuangu Biotechnology Co., Ltd., product number AB-038B), and the coating concentration was 0.2 mg / mL. According to the method in Example 1, the cardiac troponin I antibody (purchased from Huakui Jinpei, product number F6B11) and chicken IgY (purchased from Wuhan Yuangu Biotechnology Co., Ltd., product number BCB005) were respectively labeled with biotin and fluorescent Coupling with streptavidin, mixed and sprayed on the glass ...
Embodiment 3
[0080] Embodiment 3 adopts kit of the present invention to detect serum sample
[0081] Collect the serum samples of 110 patients with chest pain, use the kit of the present invention to detect the cTnI concentration in the samples, and adopt the Beckman Coulter ACCESS 2 automatic immune analysis system to detect the cTnI concentrations in these samples, and compare the data correlation between the two , the result is as Image 6 .
[0082] Collect the serum samples of 110 chest pain patients, use the kit of the present invention to detect the BNP concentration in the samples, and adopt the Beckman Coulter ACCESS 2 automatic immune analysis system to detect the BNP concentrations of these samples at the same time, compare the data correlation of the two, the result Such as Figure 7 .
[0083] Collect the serum samples of 110 chest pain patients, use the kit of the present invention to detect the concentration of D-dimer in the sample, and adopt Siemens automatic chemilumin...
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