Triple immunofluorescence quantitative detection kit for cardiac troponin I, brain natriuretic peptide and D-dimer chest pain

A technique for cardiac troponin and brain natriuretic peptide, which is applied in biological testing, measuring devices, material testing products, etc., can solve problems such as lack of detection kits, and achieve the effects of improving sensitivity, uniform flow rate, and uniform detection line

Pending Publication Date: 2021-02-05
RELIA BIOTECH JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the detection kits for cTnI, BNP and D-dimer in the prior art exist in the form of single detection or double detection, and there is a lack of detection kits that can simultaneously detect cTnI, BNP and D-dimer with high sensitivity

Method used

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  • Triple immunofluorescence quantitative detection kit for cardiac troponin I, brain natriuretic peptide and D-dimer chest pain
  • Triple immunofluorescence quantitative detection kit for cardiac troponin I, brain natriuretic peptide and D-dimer chest pain
  • Triple immunofluorescence quantitative detection kit for cardiac troponin I, brain natriuretic peptide and D-dimer chest pain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Kit for joint quantitative detection of cardiac troponin I, brain natriuretic peptide and D-dimer

[0028] 1. Composition and preparation method of the kit

[0029] A kit for the joint quantitative detection of cardiac troponin I, brain natriuretic peptide and D-dimer, including: a test card, a U disk with a standard curve and a sample diluent.

[0030] (1) Structure of test card

[0031] combined figure 1 with figure 2 , illustrating the structure of the test card.

[0032] The test card includes a card shell and a test strip inside the card shell.

[0033] The test strip includes a substrate 6 and a sample pad 7 , a CP pad 8 , an NC film 9 and an absorbent pad 10 pasted on the substrate in sequence. Adjacent ends of the sample pad, CP pad, NC membrane, and absorbent pad overlap each other.

[0034] The cartridge includes an upper cartridge 1 and a lower cartridge 2 that are engaged with each other. The inner surface of the lower cartridge 2 is provide...

Embodiment 2

[0071] Embodiment 2 The effect contrast of test strip of the present invention and other test strips

[0072] Control test strips 1-4 were prepared respectively.

[0073] The NC film of the control test strip 1 is provided with T2 detection lines and quality control lines at equal intervals. The T2 detection line is coated with cTnI monoclonal antibody (purchased from Huakui Jinpei, product number A8H12), and the coating concentration is 1 mg / mL. The quality control line was coated with rabbit anti-chicken IgY monoclonal antibody (purchased from Wuhan Yuangu Biotechnology Co., Ltd., product number AB-038B), and the coating concentration was 0.2 mg / mL. According to the method in Example 1, the cardiac troponin I antibody (purchased from Huakui Jinpei, product number F6B11) and chicken IgY (purchased from Wuhan Yuangu Biotechnology Co., Ltd., product number BCB005) were respectively labeled with biotin and fluorescent Coupling with streptavidin, mixed and sprayed on the glass ...

Embodiment 3

[0080] Embodiment 3 adopts kit of the present invention to detect serum sample

[0081] Collect the serum samples of 110 patients with chest pain, use the kit of the present invention to detect the cTnI concentration in the samples, and adopt the Beckman Coulter ACCESS 2 automatic immune analysis system to detect the cTnI concentrations in these samples, and compare the data correlation between the two , the result is as Image 6 .

[0082] Collect the serum samples of 110 chest pain patients, use the kit of the present invention to detect the BNP concentration in the samples, and adopt the Beckman Coulter ACCESS 2 automatic immune analysis system to detect the BNP concentrations of these samples at the same time, compare the data correlation of the two, the result Such as Figure 7 .

[0083] Collect the serum samples of 110 chest pain patients, use the kit of the present invention to detect the concentration of D-dimer in the sample, and adopt Siemens automatic chemilumin...

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Abstract

The invention provides a triple immunofluorescence quantitative detection kit for cardiac troponin I, brain natriuretic peptide and D-dimer chest pain. The kit for joint quantitative detection of cardiac troponin I, brain natriuretic peptide and D-dimer comprises a reagent strip, and the reagent strip comprises a substrate, and a sample pad, a CP pad, an NC membrane and an absorption pad which aresequentially pasted on the substrate; the CP pad is coated with a cardiac troponin I antibody, a brain natriuretic peptide antibody, a D-dimer antibody and a fluorescein labeled conjugate of chickenIgY; and the NC membrane is respectively provided with a detection line coated with a D-dimer monoclonal antibody, a cTnI monoclonal antibody, a BNP monoclonal antibody and a rabbit anti-chicken IgY monoclonal antibody. According to the detection kit disclosed by the invention, cTnI, BNP and D-dimer can be simultaneously, quickly and accurately detected quantitatively by one-time sampling, so thatclinical diagnosis and treatment on a patient suffering from chest pain are effectively assisted.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a triple immunofluorescence quantitative detection kit for cardiac troponin I, brain natriuretic peptide and D-dimer chest pain. Background technique [0002] Fatal chest pain is a common clinical disease. Once the disease occurs, it will progress rapidly and continue to worsen. If it cannot be diagnosed and treated in time, it will lead to the death of the patient. Acute myocardial infarction (AMI), acute aortic dissection (AAD) and acute pulmonary embolism (APE) are the three most common fatal chest pain diseases in clinical practice. For fatal chest pain, the sooner rescue can save the patient's life; however, in clinical diagnosis and treatment, affected by the patient's atypical clinical manifestations and complex etiology, it often leads to misdiagnosis and missed diagnosis of the disease, delaying the timely treatment of patients; therefore, in actual diagnosis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/68G01N33/577G01N33/558G01N33/58
CPCG01N33/558G01N33/577G01N33/582G01N33/6887G01N33/6893G01N33/74G01N2333/4712G01N2333/58G01N2333/75G01N2800/122G01N2800/324G01N2800/329
Inventor 朱庆平尹欢任婷陈金龙
Owner RELIA BIOTECH JIANGSU
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