Method for assembling and annotating Guide black fur sheep genome based on three-generation PacBio and Hi-C technologies
A genome and fur technology, applied in the field of biological information, can solve problems such as the unpublished genome of sheep, and achieve the effects of high integrity, good continuity and many repeating sequences
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[0041] Example 1. A method for assembling and annotating the guide black fur genome based on three generations of PacBio and Hi-C technology
[0042] 1 Materials and methods
[0043] 1.1 Sample collection
[0044] One healthy adult ram (altitude >3500m) was selected in Guinan County, Guinan County, Hainan Tibetan Autonomous Prefecture, Qinghai Province, and 5ml of blood was collected from the jugular vein in the morning on an empty stomach and added to a blood collection tube with EDTA-K2 anticoagulant. , stored in -20°C refrigerator. After slaughtering and immediately dividing the heart, liver, lung, lung, spleen, rumen and muscle tissue, quickly rinse the blood on the surface of the tissue with pre-cooled normal saline, and cut into 0.5cm 2 Small pieces and stored in liquid nitrogen.
[0045] 1.2 Library Construction and Sequencing
[0046] The blood genome extraction kit (Tiangen Biochemical Technology Beijing Co., Ltd., Beijing) and the CTAB (cetyltrimethylammonium bro...
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