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A kind of detection method of tetracycline

A detection method, tetracycline technology, applied in the detection field, can solve problems such as time-consuming and complicated, expensive equipment, and unfriendly organic solvents, and achieve the effect of fast detection method and high detection sensitivity

Active Publication Date: 2022-06-24
TCL CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a detection method for tetracycline, which aims to solve the problem that the existing method requires expensive instruments and equipment, time-consuming and complicated pretreatment procedures, and uses some organic compounds in the detection process. The problem of solvents being unfriendly to the environment

Method used

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  • A kind of detection method of tetracycline
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Examples

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Effect test

Embodiment 1

[0034] Example 1: MoS 2 The above synthesis method is described in detail by taking nanosheets as an example.

[0035] A kind of MoS with 6 layers was prepared 2 Nanosheets, dissolved in Tris-HCl buffer solution;

[0036] A CdSe quantum dot whose surface is modified with hydroxyl group is prepared;

[0037] Prepare a tetracycline aptamer single-stranded DNA: -HOOC-CGT ACG ATG CAG-COOH;

[0038] Dissolve -HOOC-CGT ACG ATG CAG-COOH and CdSe quantum dots modified with hydroxyl groups on the surface in a pH=6.0, Tris-HCl buffer solution, and stir for 2 hours to obtain a quantum dot-aptamer solution;

[0039] lamellar MoS 2 The solution is added to the quantum dot-aptamer solution to quench the fluorescence of the quantum dot-aptamer;

[0040] The tetracycline solution was then added to the above solution, and after incubation at room temperature for 30 minutes, the change in the fluorescence intensity of the system was measured, and it was detected that the system had fluoresce...

Embodiment 2

[0041] Example 2: The following is a VTe 2 The above synthesis method is described in detail by taking nanosheets as an example.

[0042] A 10-layer VTe was prepared 2 Nanosheets, dissolved in phosphate buffered solution;

[0043] An InAsP quantum dot whose surface is modified with amino groups is prepared;

[0044] An aptamer single-stranded RNA for tetracycline was prepared: -HOOC-CGT ACG CUA UAG CGU CAG-COOH;

[0045] Add -HOOC-CGT ACG ATG CAG-COOH and InAsP quantum dots modified with amino groups on the surface to dissolve in PH=7.0, phosphate buffer solution, and stir for 20 hours;

[0046] The lamellar VTe 2 The solution is added to the quantum dot-aptamer solution to quench the fluorescence of the quantum dot-aptamer;

[0047] The tetracycline solution was then added to the above solution, and after incubation at room temperature for 60 minutes, the change of the fluorescence intensity of the system was measured, and it was detected that the system had fluorescence...

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Abstract

The invention discloses a tetracycline detection method. The detection method of described tetracycline comprises the steps: provide quantum dot-aptamer solution, in described quantum dot-aptamer, quantum dot is combined with aptamer, and described aptamer is single-stranded DNA or single-stranded RNA; The quantum dot-aptamer is combined with a layered transition metal compound; the solution to be tested is added to the solution, and if the solution shows fluorescence, it is detected that the solution to be tested contains tetracycline. In the method provided by the invention, the transition metal compound with a layered structure is used as a "biological switch" and the quantum dot is used as a fluorescent marker to detect tetracycline. The detection method of the invention is fast and has high detection sensitivity.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a detection method of tetracycline. Background technique [0002] Tetracycline (TET) is a common antibiotic, which is widely used in human diseases and pharmaceutical additives due to its advantages of good antibacterial properties, low price and low side effects. However, due to the excessive abuse of TET and the high solubility of TET in water, it has caused serious pollution to surface water and groundwater. Residual tetracycline can be passed to the human body through the food chain. With the accumulation of tetracycline in the human body, it will produce a series of side effects, such as bacterial resistance, causing liver damage, affecting bone development, weakening the function of the human immune system, etc. . [0003] In recent years, two-dimensional (2D) transition metal nano-quantum dot materials with lamellar structures, including transition metal dichalcogenide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 邓承雨芦子哲
Owner TCL CORPORATION
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