Inclined top-type static Bessel light sheet imaging system

Abstract

The invention discloses an inclined top-type static Bessel light sheet imaging system, which belongs to the field of biomedical microscopic imaging, and comprises a generating and shaping unit for generating a Gaussian beam and shaping and expanding the Gaussian beam into a line focusing beam; a Bessel light sheet generation unit which comprises a double-ring mask and is used for modulating the intensity of the line focusing light beam to obtain a segmented line light source; an illumination unit which is used for converging the segmented line light sources into a low-sidelobe Bessel beam and obliquely emitting the low-sidelobe Bessel beam into a sample chamber which is horizontally arranged so as to excite and generate fluorescence in a sample; a detection unit which is orthogonal to the illumination unit, is inclined relative to the horizontal plane, and detects fluorescence; an aberration correction unit that corrects the fluorescence; an imaging unit which generates an image. An inclined top-type imaging system is formed in an illumination and detection mode inclined with the horizontal plane, a transparent large sample can be imaged, the low-sidelobe Bessel beam formed by the double-ring mask solves the problem that the sidelobe affects the image contrast and photobleaching, and large-view-field and high-resolution imaging is achieved.

Description

technical field [0001] The invention belongs to the field of biomedical microscopic imaging, and more particularly relates to an oblique top-mounted static Bessel light sheet imaging system. Background technique [0002] Optical microscopy imaging system is an emerging biomedical imaging system, and its illumination light path is usually perpendicular to the detection light path. The illumination light path usually uses optical elements to shape the laser beam to form a sheet-like beam that excites the fluorescent signal of the sample from the side. The detection optical path typically images the sample in a CCD or sCMOS in a direction perpendicular to the illumination optical path. According to the properties of the beam, the imaging light sheet can be divided into Gaussian light sheet and Bessel light sheet. The Gaussian optical sheet is generally formed by one-dimensional compression of the Gaussian beam by a cylindrical lens. The smaller the thickness of the Gaussian o...

AI Technical Summary

Problems solved by technology

The sample of the horizontally arranged light sheet imaging system is placed vertically on the intersection of the illumination light path and the detection light path through the designed holder. Due to the narrow sample operation space, this arrangement cannot achieve high-throughput imaging.

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