An oblique top-mounted static Bessel light sheet imaging system

Abstract

The invention discloses an oblique top-type static Bessel light sheet imaging system, which belongs to the field of biomedical microscopic imaging. A light sheet generation unit, including a double-ring mask, modulates the intensity of the line-focused beam to obtain a segmented line light source; an illumination unit, which converges the segmented line light source into a low-sidelobe Bessel beam and is obliquely incident on a horizontally placed sample The chamber is used to excite the sample to generate fluorescence; the detection unit is orthogonal to the illumination unit, and both are inclined relative to the horizontal plane, to detect the fluorescence; the aberration correction unit is to correct the fluorescence; the imaging unit is to generate an image. The illumination and detection method inclined with the horizontal plane forms an oblique top-top imaging system, which can image large transparent samples. The low sidelobe Bessel beam formed by the double-ring mask solves the problem of sidelobes affecting image contrast and photobleaching. Realize large field of view and high-resolution imaging.

Description

technical field [0001] The invention belongs to the field of biomedical microscopic imaging, and more particularly relates to an oblique top-type static Bessel light sheet imaging system. Background technique [0002] Optical microscopy imaging system is an emerging biomedical imaging system, and its illumination light path is usually perpendicular to the detection light path. The illumination light path usually uses optical elements to shape the laser beam to form a layer of sheet-like beams that excite the fluorescent signal of the sample from the side. The detection light path typically images the sample in a CCD or sCMOS in a direction perpendicular to the illumination light path. According to the beam properties, imaging light sheets can be divided into Gaussian light sheets and Bessel light sheets. The Gaussian light sheet is generally formed by one-dimensional compression of the Gaussian beam by a cylindrical lens. The smaller the thickness of the Gaussian light she...

AI Technical Summary

Problems solved by technology

The sample of the horizontally arranged light sheet imaging system is placed vertically on the intersection of the illumination light path and the detection light path through the designed holder. Due to the narrow sample operation space, this arrangement cannot achieve high-throughput imaging.

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