Molecular imaging method based on high-stability capillary electrophoresis separation analysis technology

A technology of capillary electrophoresis and molecular imaging, which is applied in the fields of life sciences, pharmaceutical development and materials science, clinical medicine, and analytical chemistry. It can solve the problems of difficult application of mass spectrometry imaging, high operation requirements, and high maintenance costs, and achieve wide separation of objects and easy operation. Simple, easy-to-scale effects

Active Publication Date: 2021-07-13
INST OF CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still many problems or challenges in mass spectrometry imaging. For example, components that are difficult to analyze, difficult to ionize, and components with the same mass/charge ratio cannot be mapped by mass spectrometry i

Method used

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  • Molecular imaging method based on high-stability capillary electrophoresis separation analysis technology
  • Molecular imaging method based on high-stability capillary electrophoresis separation analysis technology
  • Molecular imaging method based on high-stability capillary electrophoresis separation analysis technology

Examples

Experimental program
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Embodiment 1

[0055] 1. Use a grid knife to perform pretreatments such as uniform cutting, in situ extraction, and fluorescence derivatization of tissue sections

[0056] Such as figure 1 The schematic diagram of the operation of the sample pretreatment method of the grid knife tissue section is shown, taking the mouse brain tissue section as an example, and the specific operation includes the following steps:

[0057] 1. Lay freshly obtained cryosections of mouse brain tissue on a clean parafilm.

[0058] 2. Trim the parafilm to a size that matches the size of the slicing groove, and place it on the bottom of the slicing groove with two layers of sealing film as a whole, add the hydrophobized grid blade, and press down to make the slice evenly Divide and compartmentalize into miniature square wells.

[0059] 3. There is no need to transfer the cut slices, because the micro-wells of the grid knife are like micro-containers, which play a role of separation. Add 1.5 μL reagent directly to ...

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Abstract

The invention discloses a molecular imaging method based on a high-stability capillary electrophoresis separation analysis technology, and relates to multiple fields of analytical chemistry, life science, clinical medicine, medicine development, material science and the like. The molecular imaging method comprises the steps of sample pretreatment, high-stability capillary electrophoresis sample introduction, separation and analysis and molecular distribution image drawing. The sample pretreatment adopts a grid knife method, and comprises the steps of uniform segmentation, in-situ extraction, enrichment, derivation and the like. According to the method, the strong separation capability and abundant separation modes of capillary electrophoresis are utilized, imaging elements are abundant, and neutral molecules difficult to distinguish by mass spectrometry and components with the same mass-to-charge ratio can be imaged. The batch processing capacity of capillary array electrophoresis can accelerate the separation process and shorten the imaging analysis time. By means of the high-stability capillary electrophoresis technology, the peak position of the separated component can be stabilized, identification and positioning of the component are facilitated, and then a spatial distribution image of the stable peak component is constructed.

Description

technical field [0001] The present invention relates to various fields such as analytical chemistry, life science, clinical medicine, medicine development and materials science, and specifically relates to a molecular imaging method based on high-stable capillary electrophoresis separation and analysis technology. Background technique [0002] Imaging technology is a high-throughput, multi-parameter (or multi-parameter), multi-level, visualized data analysis and presentation method. Through imaging, we can intuitively and quickly read a large amount of information. The imaging range is very wide, ranging from celestial bodies, galaxies, and the universe to cells, molecules, and even atoms. However, most of the current imaging methods are based on a certain property of matter, such as luminescence, light transmission, light reflection, magnetism, force, etc., and usually can only give the shape corresponding to this property of the observed object. or contour images, which ...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/447
Inventor 陈义郑清锋许吉英郭振朋
Owner INST OF CHEM CHINESE ACAD OF SCI
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