Balanophora involucrata extract as well as preparation method and application thereof

A technology of extract and deer grass, applied in the field of whitening plant extract and preparation thereof, can solve the problems of anti-arrhythmia and the like, and achieve the effect of good whitening activity

Pending Publication Date: 2021-08-06
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effects on the human body include promoting collagen synthesis, anti-fatigue, anti-arrhythmia; lowering blood fat, inhibiting blood sugar rise; promoting cell growth, stimulating dermal cell division, and eliminating cholesterol in the body; anti-tumor activity; Pain and other physiological activities, but there is no report that it has whitening activity

Method used

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  • Balanophora involucrata extract as well as preparation method and application thereof
  • Balanophora involucrata extract as well as preparation method and application thereof
  • Balanophora involucrata extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation of embodiment 1 deer grass extract

[0017] (1) Place deer grass powder in 40% ethanol solution and extract by percolation method to obtain ethanol extract, recycle ethanol solvent to obtain the first extract; (2) add a small amount of water to the first extract , forming a suspension, adjusting the pH value of the suspension between 4.5-6.0; (3) adding an appropriate amount of biological enzymes to the suspension, and reacting in a water bath at 45°C for 60 minutes; (4) transferring the reaction solution to a Soxhlet extractor, adding 60% Ethanol, leaching for 90min, filtered and separated after cooling, and the filtrate was concentrated to prepare the target extract (hereinafter referred to as sample 1).

Embodiment 2

[0018] Embodiment 2 comparison of different preparation methods of deer grass extract

[0019] Place deer grass powder in 60% ethanol solution and extract by percolation method to obtain ethanol extract, recycle ethanol solvent to obtain the first extract; (2) add a small amount of water to the first extract to form a suspension solution, adjust the pH value of the suspension between 4.5-6.0; (3) add an appropriate amount of biological enzymes to the suspension, and react in a water bath at 45°C for 60 minutes; (4) transfer the reaction solution into a Soxhlet extractor, add 60% ethanol, soak Extract for 90min, filter and separate after cooling, and concentrate the filtrate to prepare the target extract (hereinafter referred to as sample 2)

[0020] Place deer grass powder in 40% ethanol solution and extract by percolation method to obtain ethanol extract, recycle ethanol solvent to obtain ethanol sample (hereinafter referred to as sample 3)

[0021] Place deer grass powder i...

Embodiment 3

[0029] Embodiment 3 different preparation methods tyrosinase activity comparison

[0030] As a person skilled in the art, everyone knows that the parts extracted by different extraction methods contain different compounds and have different activities. On the premise that all the chemical components are not clear, the activities of the samples prepared in Example 1 and Example 2 were compared through the tyrosine activity.

[0031] Determination of tyrosinase (TYR) activity: Measure 3mL of 50mmol / L PBS (pH=6.8), add 2.9mL of 1mmol / L levodopa solution, place it in a water bath at 32°C for 20min, add 0.1mL of TYR solution (0.01g / L) was mixed, and the growth curve of the absorbance of the mixed solution with time was measured under constant temperature conditions. It is stipulated that the absorbance value of the reaction solution at a wavelength of 475nm increases by 0.001 per minute as a TYR activity unit (U / min), and kojic acid is used as a positive control.

[0032] Calcula...

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Abstract

The invention provides a rhaponticum aestivum extract as well as a preparation method and application thereof, and belongs to the technical field of plant extracts. The rhaponticum aestivum extract comprises the following components: 1.92 to 10.38 mg/g of 3-methoxy-quercetin, 1.50 to 6.47 mg/g of 3,4-dihydroxy benzoic acid, 1.32 to 3.79 mg/g of apigenin, 1.97 to 8.59 mg/g of kaempferol and 25 to 500 [mu]g/g of rhaponticum aestivum sterone, and the rhaponticum aestivum extract is prepared by a percolation assisted enzymolysis process. The rhaponticum aestivum extract disclosed by the invention can achieve a whitening effect by inhibiting the activity of tyrosinase.

Description

technical field [0001] The invention belongs to the technical field of plant extracts, and in particular relates to a whitening plant extract and its preparation method and application. Background technique [0002] With the improvement of people's living standards and aesthetic standards, smooth and fair skin is more and more respected by women, and the proportion of whitening products in the daily chemical product market is also increasing. Due to the safe and healthy performance of natural functional substances, daily chemical products containing natural plant whitening functional ingredients have gradually become the consumer objects that people respect and the product orientation of the industry. The commonly used commercialized natural whitening extract is glabra root extract , peony root extract and tea extract etc. [0003] Tyrosinase inhibitors from natural sources are attracting more and more attention due to their safety, effectiveness, and ability to effectively...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/49A61K8/63A61K8/368A61Q19/02
CPCA61K8/9789A61Q19/02A61K8/498A61K8/368A61K8/63A61K2800/782A61K2800/805A61K2800/5922
Inventor 韦敏汤毅宋萍萍汤承翰颜露钱怡云王晨
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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