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Methods, devices, kits and compositions for detecting tapeworm

一种绦虫、复合物的技术，应用在区分绦虫抗原的抗体和抗体组合物领域，能够解决复杂、程序困难、不易操纵等问题

Pending Publication Date: 2021-08-06
IDEXX LABORATORIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hygienic and harmless procedures for disposing of feces are not easily manipulated and often complex
Such procedures can include weighing, centrifugation, and storage and are difficult except in clinical laboratories equipped with appropriate instrumentation, protective equipment, and skilled technicians

Method used

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  • Methods, devices, kits and compositions for detecting tapeworm
  • Methods, devices, kits and compositions for detecting tapeworm
  • Methods, devices, kits and compositions for detecting tapeworm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment A

[0142] Unless otherwise indicated, the following materials and techniques were used to generate the data described in one or more of Examples 1-5 as described below.

[0143] Preparation of Worm Extracts from Taenia fasciatus and Dipylidium caninum . Worm extracts of Taenia fasciatus and Dipylidium caninum were purchased from Antibody systems, Inc., Hurst, Texas, U.S.A. Worm extracts were centrifuged at 10,000 g for 30 min at 4 °C. Supernatants were collected, dialyzed into PBS, pH 7.0 (membrane molecular weight cut-off 12-14 kD, Part 132678, Spectrum, Repligen, Waltham MA, USA), and protein concentrations were determined using the Bradford assay.

[0144] Preparation of Worm Extracts from Taenia gigantea. C. gigantea was obtained from Ross University School of Veterinary Medicine, Saint Kitts. Whole worms were washed several times with cold PBS (pH 7.0) at room temperature to remove fecal material and mucus from the host and homogenized with a tissue grinder at 4 °C un...

Embodiment 1A

[0155] Generation and screening of murine monoclonal antibodies against Taenia lentils. Murine mAbs (monoclonal antibodies) were produced by immunizing mice with worm extracts (WE) of Taenia luteiformis (hereafter referred to as Taenia phagiformis extracts or . tumor. In an ELISA capture assay, candidate hybridomas were screened for the ability of their secreted mAbs to bind the immunogen (ie, an extract of Taenia fauna) coated on a microtiter plate. From this screen, one hundred (100) hybridomas (ie, 100 mAbs) that were positive in the screen (ie, able to bind the Taenia luteum extract) were selected for further analysis. Screened by capture assays, the 100 candidate mAbs were further screened for their ability to function in a fecal antigen ELISA assay. Microtiter plates were coated with rabbit polyclonal antibodies raised by immunizing rabbit standards with extracts of Taenia fabaciformis. FEX (fecal extract) prepared from canine excrement samples was added. The faecal...

Embodiment 1B

[0159] Specificity and Sensitivity Evaluation of ELISA for Taenia faeciformis Fecal Antigens. To assess the specificity of the assay, it was tested against the FEX versus Taenia faeciformis fecal antigen ELISA assay (using ADX131 and ADX132-HRP conjugates coated on plates) from: Infected with hookworm Ancylostoma canis ( Canidae of the roundworm Toxocara canis (n=9), the roundworm T. canis (n=9), the whipworm Trichocephalis (n=36), Dipyridium caninum (n=44) or Taenia lentilis (n=5) animal. Results (see image 3 ) shows that all 5 samples of Taenia fauna in this assay were positive, while all other samples were negative. The Taenia faeciformis fecal antigen ELISA did not cross-react with the hookworm Ancylostoma canis, the roundworm Toxocara canis, the whipworm Trichocephala vulpes, or Dipyridium canis. Therefore, in this experiment, the ELISA assay for Taenia faeciformis coproantigen had 100% sensitivity and specificity. To assess the specificity of the assay, a sandwich ...

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PUM

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Abstract

Methods, devices, kits and compositions for detecting the presence or absence of one or more tapeworm coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of tapeworm in a fecal sample from a mammal and may also be able to distinguish between different tapeworm species and in tire presence of one or more infections with helminths (such as roundworm, hookworm, whipworm and heartworm), Giardia and parvovirus. Confirmation of the presence or absence of tapeworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and / or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

Description

[0001] cross reference [0002] This application claims priority to U.S. Provisional Application Serial Nos. 62 / 740,100 (filed October 2, 2018); 62 / 741,849 (filed October 5, 2018); and 62 / 746,805 (filed October 17, 2018) interests, are incorporated by reference in their entirety. technical field [0003] The present invention relates to compositions, devices, kits and methods for detecting and distinguishing Taenia species in mammals. More specifically, the present invention relates to antibodies and antibody compositions, devices, kits and methods for detecting the presence or absence of Taenia species in a sample from a mammal and for distinguishing Taenia antigens. Background technique [0004] Parasitic helminth infections are common in animals and can cause serious illness or death if left undiagnosed and untreated. Current methods for diagnosing parasitic helminth infections primarily consist of microscopic examination of fecal samples either directly in fecal smears...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70G01N33/53
CPCC07K16/18G01N33/5308G01N2333/43539G01N33/535G01N33/54386C07K2317/33G01N33/563
Inventor 耿进明大卫·艾伦·艾瑟莫尔
Owner IDEXX LABORATORIES
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