Magnetic nanoparticle visual pathogen detection kit for porcine viral diseases

Abstract

The invention discloses a magnetic nanoparticle visual pathogen detection kit for porcine viral diseases. According to the kit, an enrichment probe on the functionalized magnetic particles and a hybridization probe on the functionalized gold nanoparticles are combined with pathogenic nucleic acid in a sample to form a hybridized gold compound, and horseradish peroxidase collected by the functionalized gold nanoparticles in the hybridized compound is combined to detect the pathogenic nucleic acid in the sample; through enzymatic reaction, field visual detection on common pig pathogens is realized; the detection process does not need pathogen nucleic acid extraction, the detection result is specific, accurate and visual, the detection sensitivity can reach the PCR detection level, and the rapid pathogen detection requirement of a basic mechanism and an epidemic site without any laboratory condition can be met; therefore, a technical support is provided for early discovery, early prevention and early treatment of porcine viral infectious diseases, and economic and social significance is huge.

Description

technical field [0001] The invention belongs to the fields of nanomaterials, analytical chemistry and animal virus detection, and relates to a magnetic nanoparticle visual detection kit for pig common pathogens. Background technique [0002] Intensive pig farms are specialized pig farms that implement high-density, high-efficiency, continuous and balanced production. Intensive pig farms have extremely high requirements for feeding management and disease prevention and control. Once the pathogen invades, it is difficult to remove it. The difficulty of prevention and control lies in the mixed infection or secondary infection caused by the joint action of multiple pathogens. Its occurrence can increase the unstable factors of the pig farm, and it is easy to cause the whole pig farm to suffer from continuous disease, and even bring about a pandemic of the disease. [0003] At present, the common porcine viral diseases that endanger large-scale pig production mainly include: rep...

AI Technical Summary

Problems solved by technology

Pathogen isolation and biochemical identification are not only time-consuming and laborious, but also have poor sensitivity and specificity. Serological diagnostic methods not only cannot get results in the early stage of pig disease, but also the detection results are affected by the conditions of different laboratories

Although single PCR, partial multiplex PCR, and real-time quantitative PCR diagnostic methods for PCV2, PRRSV, PPV, CSFV, and PRV have been established at present, the complex operations such as nucleic acid extraction used in these methods need to be carried out in the laboratory, and it is impossible to quickly detect non-specific Pathogen tracking and monitoring of diseased or suspected diseased pig farms and rapid and effective pathogen on-site diagnosis of diseased pig farms

[0005] Chinese patent CN1339609A discloses nanoparticle-labeled gene probes and their preparation methods and applications. This patent realizes nano-scale amplification and visual color development through the preparation of nano-labeled gene probes, but the connection between nanoparticles and DNA has high production costs. , poor stability and inconvenient use

However, the above two patents still need to extract the nucleic acid components in the sample in the actual detection application, and need to rely on laboratory equipment, which cannot realize rapid on-site detection without instrumentation

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