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Uncooked food pesticide residue detection method

A detection method and food technology, which can be used in measurement devices, analysis by chemical reaction of materials, instruments, etc., can solve the problems of insufficient vitality and risk of test errors, and achieve the effect of good activity

Active Publication Date: 2021-08-10
华标(天津)科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for detecting pesticide residues in raw foods. The traditional enzyme inhibition method has not been immobilized with thimerosal, and the activity is insufficient, and there is a risk of error in the test.

Method used

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  • Uncooked food pesticide residue detection method
  • Uncooked food pesticide residue detection method
  • Uncooked food pesticide residue detection method

Examples

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Embodiment 1

[0038] see Figure 1-7 As shown, the present invention is a detection device for pesticide residues in raw foods. It includes a constant temperature box 100, an installation box 200, a pull bolt 300, a color test paper box 400, and a liquid outlet 500. One surface of the constant temperature box 100 is provided with a connection groove, and the installation box 200 is welded to the connection groove, and the color test paper box 400 passes through The pull bolt 300 is installed inside the installation box 200, and the liquid outlet 500 is connected with the incubator 100 through threaded screw holes;

[0039]The installation box 200 includes an installation box body and a first buffer spring 210. The color test strip box 400 includes a color development test strip body and a second slider 410. A second chute is provided on one side surface of the installation box body for color development. The test strip box 400 and the installation box 200 are slidably matched through the s...

Embodiment 2

[0045] A detection method of a detection device for pesticide residues in raw food, comprising the following steps:

[0046] Step 1: prepare buffer solution and thimerosal solution: put disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate into the first reaction kettle, add hydrochloric acid solution to adjust its pH value, obtain buffer solution, weigh a certain amount of thimerosal, Put into the second reaction kettle, add water to dissolve, obtain thimerosal solution;

[0047] Step 2: prepare acetylcholinesterase (AChE) solution: use buffer solution to dissolve electric eel acetylcholinesterase, put it into the third reaction kettle, add water to dilute to obtain acetylcholinesterase solution;

[0048] Step 3: Prepare chitosan-acetylcholinesterase membrane and add thimerosal: put the chitosan membrane into the acetylcholinesterase solution, let it absorb in the fourth reaction kettle, and then add glutaraldehyde solution to react to obtain the shell ...

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Abstract

The invention discloses an uncooked food pesticide residue detection method, and relates to the field of pesticide residue detection. The device comprises a constant-temperature box, a mounting box, a pull plug, a color developing test paper box and a liquid outlet device, wherein a connecting groove is formed in one surface of the constant-temperature box, the mounting box is welded to the connecting groove, the color developing test paper box is mounted in the mounting box through the pull plug, and the liquid outlet device is connected with the constant-temperature box in a matched mode through a thread and a screw hole. According to the invention, by arranging the liquid outlet device, enzyme liquid in a test tube can be rapidly sprayed out through extrusion of a pressing plate, a pressing rod and a piston, so that convenience and rapidness are achieved; the activity of enzyme is guaranteed by arranging the constant-temperature box; the thiomersalate is used for immobilizing the enzyme, so that the activity of the enzyme is better, and detection of pesticide residues is facilitated; and by arranging the pull plug and the mounting box, color developing test paper can be quickly taken out when needed.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection, in particular to a method for detecting pesticide residues in raw foods. Background technique [0002] Pesticide residues are the phenomenon that some pesticides remain directly or indirectly in grains, vegetables, fruits, livestock products, aquatic products, soil and water after the application of pesticides in agricultural production. If pesticide residues are eaten by people or livestock, it will cause great harm. With the improvement of people's living standards, people are very concerned about the problem of pesticide residues, and the requirements for the accuracy of detection devices are getting higher and higher. [0003] Existing pesticide residue detection devices often use high-performance liquid chromatography or enzyme inhibition method, but the traditional enzyme inhibition method has not been immobilized with thimerosal, the activity is insufficient, and there ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/80
CPCG01N21/78G01N21/80G01N2021/7759
Inventor 孔祥卉张岩
Owner 华标(天津)科技有限责任公司
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