Preparation of controlled release platelet derived growth factor nanocapsules

A platelet-derived and nanocapsule technology, which is applied in capsule delivery, nanocapsules, medical preparations of non-active ingredients, etc., can solve problems such as severe reaction conditions, influence of growth factor activity, and sudden release of drugs

Active Publication Date: 2021-08-13
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently commonly used controlled-release carriers include hydrogels, liposomes, nanoparticles, and nanofibers, etc., but these delivery carriers have more or less certain defects, such as hydrogels are easy to swell and cause sudden release of drugs; Organic solvents are often required in the process of fiber synthesis, or the reaction conditions are severe, which will affect the activity of growth factors

Method used

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  • Preparation of controlled release platelet derived growth factor nanocapsules
  • Preparation of controlled release platelet derived growth factor nanocapsules
  • Preparation of controlled release platelet derived growth factor nanocapsules

Examples

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Embodiment 1

[0036] 1. Synthesis of PLA-PEG-PLA

[0037] (1) Take a small amount of sodium block and cut it into small pieces and add it to toluene together with benzophenone, heat it to boiling with an electric heating mantle, then condense and reflux until it becomes dark blue, then distill the toluene to collect the distillate to obtain anhydrous toluene . Take an appropriate amount of stannous octoate and dissolve it in anhydrous toluene.

[0038] (2) Preparation and characterization of PLA-PEG-PLA. PLA-PEG-PLA is synthesized by ring-opening polymerization of lactide. All the reaction tubes to be used were washed and dried in an oven at 120°C for 2h. Add an appropriate amount of PEG1000 to the reaction tube, and remove the water in it by vacuuming under the condition of 100°C oil bath. After 2 hours, remove the heating device and turn off the vacuum pump. After cooling to room temperature, the purified D,L-lactide and Sn(Oct)2 toluene solution were added in proportion, and reacted ...

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Abstract

The invention discloses preparation of controlled release platelet derived growth factor nanocapsules, and belongs to the field of nanocapsules. The nanocapsules are prepared through a method of in situ free radical polymerization. Monomers and crosslinkers are enriched around single PDGF-BB through electrostatic action / hydrogen-bonding interaction;free radicals are generated under the action of an initiator (APS and TEMED); and in situ free radical polymerization is performed. Controlled release is realized by designing components (the crosslinkers) of the nanocapsules. Two crosslinkers capable of responding to pH degradation are selected but with different degradation rates. By adjusting the ratio of the two, the controlled release of PDGF-BB can be realized. The synthesized nanocapsules wrap the PDGF-BB to enhance stability; the controlled release of the PDGF-BB can be realized; the nanocapsules has significant meanings for wound repair and tissue regeneration diseases; and thoughts can be provided for the delivery of other protein drugs.

Description

technical field [0001] The invention relates to a preparation method of a platelet-derived growth factor nanocapsule for controlled release, and belongs to the field of nanocapsules. Background technique [0002] Platelet-derived growth factor (PDGF) is a cationic glycoprotein that is heat-resistant, acid-resistant, and easily hydrolyzed by proteases. It can be secreted by a variety of cells, such as platelets, fibroblasts, macrophages, etc. PDGF has four monomers PDGF-A, PDGF-B, PDGF-C, and PDGF-D, and these monomers are connected by disulfide bonds to form a dimer form. Among them, PDGF-BB, as a mitosis promoter, can stimulate the proliferation and migration of fibroblasts and smooth muscle cells, and promote the production of macrophages and the secretion of growth factors. It is more effective in wound repair, tissue regeneration, bone and tooth regeneration, and joint repair. Especially in wound repair, it is the only growth factor approved by the US Food and Drug Adm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K9/52A61K47/34A61K47/14A61P17/02A61P19/08A61P19/02A61P31/10
CPCA61K38/1858A61K9/5153A61K9/5123A61P17/02A61P19/08A61P19/02A61P31/10
Inventor 甘志华蔡素素柳朝永秦蒙喻青松
Owner BEIJING UNIV OF CHEM TECH
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