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Sansanthoxylum botanica fungicide and its preparation method and application

The technology of a plant-derived fungicide and sanshoenone is applied in the field of sanshoko plant-derived fungicide and its preparation, which can solve the problems that the anti-phytopathogenic bacteria activity of sanshoko and sanshoenone has not been reported, and achieves a good control effect. , strong bactericidal activity, broad antibacterial spectrum effect

Active Publication Date: 2022-04-08
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, a large number of medical experiments have shown that sanshollenone exhibits high-efficiency biological activities in anti-tumor and anti-tuberculosis, but the activity of sanshoperon and sanshotenone in anti-phytopathogenic bacteria has not been reported yet.

Method used

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  • Sansanthoxylum botanica fungicide and its preparation method and application
  • Sansanthoxylum botanica fungicide and its preparation method and application
  • Sansanthoxylum botanica fungicide and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The preparation of embodiment 1-sanjiaozi whole plant methanol crude extract

[0032] Cut the fresh Sansanthoxylum plant into small pieces, put it into a blast drying oven for drying at 50°C, and grind it into powder. Weigh 2.5kg of the powder and soak it in 20L of methanol solution with a volume fraction of 99.5% for 72 hours each time. The soaking liquids were combined and filtered, and concentrated under vacuum at 45°C with a rotary evaporator to obtain the methanol crude extract of the whole plant of Sanshochu.

Embodiment 2

[0033] The preparation of embodiment 2-sanjiaozi whole plant methanol crude extract

[0034] Cut the fresh Sansanthoxylum plant into small pieces, dry them in a blast drying oven at 48°C, and grind them into powder. Weigh 2kg of the powder and soak them in 20L of methanol solution with a volume fraction of 99%. The liquid was combined and filtered, and concentrated under vacuum at 40°C with a rotary evaporator to obtain a methanol crude extract of the whole plant of Sanshochu.

Embodiment 3

[0035]The preparation of embodiment 3-sanjiaozi whole plant methanol crude extract

[0036] Cut the fresh Sansanthoxylum plant into small pieces, put it in a blast drying oven to dry at 52°C, and grind it into powder. Weigh 3kg of the powder and soak it in 20L of methanol solution with a volume fraction of 99.5% for 75 hours each time. The solution was combined and filtered, and concentrated under vacuum at 50°C with a rotary evaporator to obtain crude methanol extract of the whole plant of Sanshochu;

[0037] (1) In vitro antibacterial activity test

[0038] The mycelia growth rate method was used to detect the bactericidal activity of the methanol crude extract of Sansanthoxylum bungeanum obtained in the above Examples 1-3.

[0039] Take the methanolic crude extracts of peppercorns from Examples 1-3 and dissolve them in DMF (0.1 mL), then mix them with potato dextrose agar (PDA; 9.9 mL), and finally prepare the required concentration. The crude extract was tested for activ...

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Abstract

The present invention provides a plant-derived fungicide of Sansanthoxylum japonicus and its preparation method and application. The broad-spectrum plant-derived fungicide provided by the invention includes: the whole plant extract of Sansanthoxylum japonicus and the compound sanshovenone obtained by separation and purification have antibacterial spectrum Wide range, simple separation process and other advantages. The potted bactericidal activity test proves that the Sansanthoxylum extract has a good control effect on pepper phytophthora, cucumber downy mildew, potato late blight, and cowpea powdery mildew, and has a promoting effect on cucumber growth. The control effect of sanshocenone on cucumber downy mildew is equivalent to that of the chemical fungicide cyazofamid. At the same time, the field efficacy test proves that the fungicidal activity of sanshoperone extract on cucumber powdery mildew is better than that of the commercial fungicide Lunason. As a potential botanical fungicide for the prevention and control of plant diseases.

Description

technical field [0001] The invention relates to the technical field of fungicides, in particular to a plant-derived fungicide from Sansanthoxylum bungeanum and its preparation method and application. Background technique [0002] With the improvement of people's awareness of environmental protection, the voice of advocating nature, protecting the environment, and paying attention to food safety is increasing day by day. Pollution-free biological pesticides and biological control technologies and concepts have gradually attracted people's attention. Natural fungicides isolated from plants have been successfully used to control plant diseases, and have advantages over chemically synthesized pesticides, such as containing new compounds that are not yet resistant to known plant pathogenic fungi, relatively fast biodegradation, and less residue , renewable materials, etc. At present, many plant extracts have been proven to have fungicidal activity against phytopathogenic fungi, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/08A01N37/10A01P3/00C07C67/48C07C69/78
CPCA01N65/08A01N37/10C07C67/48A01N65/00C07C69/78A01P3/00A01N37/08
Inventor 孙然锋贺建国胡展谢佳
Owner HAINAN UNIV
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