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Multigene construct for immune-modulatory protein expression and methods of use

An expression vector and actin technology, which can be used in chemical instruments and methods, gene therapy, mammalian proteins, etc., and can solve problems such as insufficient production of immunomodulatory proteins.

Pending Publication Date: 2021-08-24
安克塞克医疗公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current expression plasmid constructs are insufficient to adequately produce each immunomodulatory protein

Method used

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  • Multigene construct for immune-modulatory protein expression and methods of use
  • Multigene construct for immune-modulatory protein expression and methods of use
  • Multigene construct for immune-modulatory protein expression and methods of use

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Embodiment

[0141] List of examples

[0142] The subject matter disclosed herein includes, but is not limited to, the following examples.

[0143] 1. An expression vector, said expression vector comprising the nucleic acid sequence of SEQ ID NO:1.

[0144] 2. An expression vector comprising a nucleic acid encoding a polypeptide comprising amino acids at least 70% identical to the amino acid sequence of SEQ ID NO:9.

[0145] 3. The expression vector according to embodiment 2, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:9.

[0146] 4. The expression vector of embodiment 2 or 3, wherein the nucleic acid comprises a nucleotide sequence having at least 70% identity to the nucleotide sequence of SEQ ID NO:8.

[0147] 5. The expression vector according to embodiment 4, wherein the nucleic acid comprises the nucleotide sequence of SEQ ID NO:8.

[0148] 6. The expression vector of embodiment 4 or 5, wherein said nucleic acid is operably linked to a nucleic acid enco...

example

[0254] I. General method.

[0255] Standard methods in molecular biology are described. Maniatis et al., (1982) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY; Sambrook and Russell, Molecular Cloning (2001), 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY; Wu, (1993) Recombinant DNA, Vol. 217, Academic Press, San Diego, CA . Standard methods are also described in: Ausbel et al., "Current Protocols in Molecular Biology" (2001), Volumes 1 to 4, John Wiley and Sons, Inc. Inc.), New York, NY, which describes cloning of bacterial cells and DNA mutagenesis (vol. 1), cloning of mammalian cells and yeast (vol. 2), glycoconjugates, and protein expression (vol. 3) and Bioinformatics (Volume 4).

[0256] Methods for protein purification are described, including immunoprecipitation, chromatography, electrophoresis, centrifugation and crystallization. Coligan et al. (2000) Current Protocols in ProteinScience, Vo...

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Abstract

Provided are expression vector constructs encoding IL-12 p35 and IL-12 p40 proteins where each protein or component thereof can be expressed utilizing appropriate promoters and / or translation modifiers. Also provided are methods of use for the expression vectors.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application Serial No. 62 / 778,027, filed December 11, 2018, which is incorporated herein by reference. [0003] References to Sequence Listings [0004] Submit as text file via EFS WEB [0005] The electronically submitted Sequence Listing is hereby incorporated by reference in its entirety (file name: 541462_SequenceListing_ST25.txt; date created: December 10, 2019; file size: 57KB). technical field [0006] Recombinant expression vectors for the intratumoral delivery of three genes encoding therapeutically active multimeric and fusion polypeptides are described. Nucleic acids encoding polypeptides isolated from translational regulatory elements are provided. Methods of delivery are also provided. Background technique [0007] Escherichia coli (E. coli) plasmids have long been an important source of recombinant DNA molecules used by researchers and industry. P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/63C12N15/85A61K41/00A61K48/00A61P35/00
CPCA61P35/00C12N15/85A61K48/0075A61K48/005A61K38/00A01K2227/105A01K2267/0331A01K2207/12C07K14/5434C07K14/475C07K14/52C07K14/4748A61K41/00A61K48/00C12N15/63A61K41/0047
Inventor 戴维·A·坎顿
Owner 安克塞克医疗公司
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