Nasal cavity allergen safety detection method and detection device

Abstract

The invention belongs to the technical field of biology and new medicine, and particularly relates to a nasal cavity allergen safety detection method which comprises the following steps of adding a sample to be detected into a solid-phase carrier coated with an inhalation allergen, adding a biotin-labeled anti-human IgE antibody into the washed solid-phase carrier, adding an enzyme-labeled avidin solution into the washed solid-phase carrier, and adding a substrate developing solution. According to the nasal cavity allergen safety detection method, the specific IgE in serum of a patient is determined by adopting an enzyme-linked immunoassay principle and an indirect method, so that the problems of limited research range and relatively small research scale in the existing detection technology are solved, the detection accuracy is improved, effective prevention measures are taken, and the method is suitable for popularization and application; the device is convenient to operate, the detection accuracy is improved, the detection difficulty is reduced, the detection cost is reduced, and the device can be adjusted according to actual detection requirements and is wide in application range.

Description

technical field [0001] The invention belongs to the technical field of biology and new medicine, and in particular relates to a safety detection method for nasal allergens, and also relates to a safety detection device for nasal allergens. Background technique [0002] Allergic reaction refers to the response of tissue damage or dysfunction that occurs when the immune body is stimulated again by the same antigen. [0003] The respiratory tract includes the mouth, nose, and throat. Allergy is a disease of immune system disorders. Inhalation allergy is an allergic reaction due to inhalation of allergens. The common clinical respiratory allergies mainly include hay fever and allergic rhinitis. , allergic cough, allergic asthma, etc., foreign substances that cause allergies are called allergens. When allergens invade the human body, the body’s immune system will release a large number of inflammatory chemicals, causing telangiectasia and blood vessel walls. When the permeabilit...

AI Technical Summary

Problems solved by technology

[0004] Since anaphylaxis is a systemic immune response, the clinical manifestations of allergies are complex and diverse. Mild respiratory allergies manifest as repeated coughing, sneezing, runny nose, nasal congestion, etc., similar to a cold; severe ones may manifest as chest tightness , poor breathing, asthma, allergies can lead to repeated respiratory infections, because allergies can increase the secretion of the respiratory mucosa, excessive secretions can easily make bacteria attach to the respiratory tract, increasing the chance of infection, and repeated infections will destroy the respiratory tract on the one hand The anti-infection ability of the body can cause a vicious circle; on the other hand, it can destroy the structure of the respiratory tract, cause organic lesions, and lead to the occurrence of asthma

[0005] Skin prick test and serum IgE detection are relatively common allergen detection methods at present. The skin prick test requires high technical requirements, and the results are easily affected by skin conditions, drugs and subjective factors. At the same time, it is inconvenient for children to operate

[0006] IgE detection is not affected by subjective factors, has lower operational technical requirements, higher safety performance, higher sensitivity and specificity, but its price is relatively expensive, and the detection accuracy is not high

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