Micro-fluidic chip for nucleic acid extraction amplification detection

Abstract

The invention provides a micro-fluidic chip for nucleic acid extraction amplification detection. The micro-fluidic chip is provided with a sample cracking purification cavity, a nucleic acid extraction cavity, a transfer channel, a nucleic acid release cavity, a magnetic microsphere collection cavity, a liquid separation structure, a waste liquid cavity and amplification reaction cavities, wherein a nucleic acid solution purified in the sample cracking purification cavity flows into the nucleic acid extraction cavity, and magnetic microspheres are specifically combined with nucleic acid in a low-pH-value solution environment of the nucleic acid extraction cavity; through magnetic field control, the magnetic microspheres are transferred to the nucleic acid release cavity, and nucleic acid is released in a high-pH-value solution environment; and an amplification preparation solution containing nucleic acid is subjected to magnetic adsorption by the magnetic microsphere collection cavity to remove the magnetic microspheres, and then is distributed to the multiple amplification reaction cavities through the liquid separation structure for a constant-temperature amplification reaction and real-time fluorescence detection. The micro-fluidic chip can realize quick and convenient nucleic acid extraction, simple and stable isothermal amplification and accurate and reliable real-time fluorescence detection, and provides a good application platform for high-throughput and efficient instant nucleic acid detection.

Description

technical field [0001] The invention relates to the field of molecular diagnosis, in particular to a microfluidic chip for nucleic acid extraction, amplification and detection. Background technique [0002] Microfluidic technology is a technology for precise manipulation of fluids on the micron scale. The microfluidic chip can take advantage of the microfluidic characteristics and chip microstructure design to realize the extraction of samples, the addition and mixing of reagents, the reaction of mixed solutions, etc., which originally required complex steps in the laboratory using professional instruments and equipment, and integrate them On a simple chip, thus completing the required biochemical analysis projects. Compared with the traditional biochemical diagnostic technology, the real-time detection technology represented by microfluidic chip technology has the following advantages: microfluidic chip and its equipment are miniaturized, easy to carry, and can be applied ...

AI Technical Summary

Problems solved by technology

[0003] However, the functions of the current microfluidic nucleic acid detection chip are basically limited to the automatic amplification and detection after nucleic acid extraction, and how to integrate the sample pretreatment, that is, the extraction of nucleic acid into the chip, has become a major problem.

Due to factors such as high cost, difficulty in industrial processing and manufacturing, complex internal structure of the chip, need for professional operation, poor stability and unification of specifications, etc., fully integrated microfluidic nucleic acid extraction and amplification detection products still face challenges. big challenge

Images