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Application of long non-coding RNA PRALR in the diagnosis of paclitaxel chemotherapy resistance in tumors

A tumor, drug resistance technology, applied in the field of biomedicine, can solve problems such as insufficient research

Active Publication Date: 2022-03-25
JINSHAN HOSPITAL FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still insufficient research on this lncRNA in the field of tumors, and there is no report

Method used

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  • Application of long non-coding RNA PRALR in the diagnosis of paclitaxel chemotherapy resistance in tumors
  • Application of long non-coding RNA PRALR in the diagnosis of paclitaxel chemotherapy resistance in tumors
  • Application of long non-coding RNA PRALR in the diagnosis of paclitaxel chemotherapy resistance in tumors

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Experimental program
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Embodiment 1

[0023] 1 material

[0024] RNAiso Plus total RNA extraction reagent (Takara 9109);

[0025] RNA Reverse Transcription Kit (ROCHE 04897030001);

[0026] SYBgreen method qPCR kit (ROCHE 04913914001);

[0027] High-fidelity enzyme PrimeSTAR Max DNA Polymerase (Takara R045A);

[0028] RACE 5' / 3' Kit (TAKARA 634858).

[0029] 2 methods

[0030] 2.1 Identification of the full-length sequence of the new transcript of TCONS_00013523

[0031] 2.1.1 Design of 5'RACE and 3'RACE amplification primers

[0032] The human lncRNA TCONS_00013523 sequence was downloaded from the UCSC database, and primers for nested PCR were designed for 5'RACE and 3'RACE amplification. The amplification primers were synthesized by Suzhou Jinweizhi Biotechnology Co., Ltd., and the primer sequences are shown in Table 1.

[0033] Table 1 Sequences of primer pairs amplified by 5'RACE and 3'RACE

[0034]

[0035] 2.1.2 5'RACE and 3'RACE amplification and sequencing

[0036] see The RACE 5' / 3'Kit (TA...

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Abstract

The present invention relates to the application of long-chain non-coding RNA PRALR-α for diagnosing tumor drug resistance to paclitaxel. In the paclitaxel-resistant ovarian cancer cells, the present invention identified a new transcript full-length sequence of lncRNA (TCONS_00013523), named PRALR-α, and found that the lncRNA was related to the resistance of various tumors to paclitaxel (PTX). The lncRNA detected in the supernatant exosomes of ovarian cancer PTX-resistant cells was significantly different between PTX-sensitive and drug-resistant cells, suggesting that it can be used as a biomarker for the diagnosis of tumor chemotherapy resistance.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a new lncRNA related to ovarian cancer drug resistance, and its use as a biomarker in preparing a diagnostic kit for tumor paclitaxel chemotherapy drug resistance. Background technique [0002] Ovarian cancer is one of the common malignant tumors of female reproductive organs, and its mortality rate ranks first among all gynecological malignant tumors. At present, paclitaxel (PTX) chemotherapy is the first-line therapy for the clinical treatment of advanced ovarian cancer, but the recurrence of ovarian cancer caused by chemotherapy resistance is still an urgent clinical problem to be solved. [0003] LncRNAs, long non-coding RNAs, are a general term for a class of RNA molecules with a length of more than 200 nt that basically have no protein-coding function. They are widely involved in the physiological and pathological processes of the body, including the occurrence and deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12N15/113C12Q2600/158C12Q2600/178C12Q2600/106C12N2310/11
Inventor 许国雄管文彩
Owner JINSHAN HOSPITAL FUDAN UNIV
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