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Complex microbial inoculant for cottonseed meal detoxification and preparation method and application thereof

A technology of compound bacterial agent and cotton meal, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve rare and other problems, and achieve the effects of easy preservation, no three wastes, and elimination of anti-nutritional factors

Pending Publication Date: 2021-09-21
NORTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few research reports on improving the nutritional quality of cottonseed meal by compound fermentation of enzyme-producing strains

Method used

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  • Complex microbial inoculant for cottonseed meal detoxification and preparation method and application thereof
  • Complex microbial inoculant for cottonseed meal detoxification and preparation method and application thereof
  • Complex microbial inoculant for cottonseed meal detoxification and preparation method and application thereof

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preparation example Construction

[0040] The present invention also provides a method for preparing a composite microbial agent for detoxification of cottonseed meal, comprising the following steps:

[0041] (1) adding bacillus to LB broth medium, carrying out shaking table vibration culture, obtaining the bacillus seed liquid used for final fermentation;

[0042] (2) Saccharomyces cerevisiae is added to the YPD liquid medium, and shaker culture is carried out to obtain the Saccharomyces cerevisiae seed liquid for final fermentation;

[0043] (3) Lactobacillus plantarum is added to MRS liquid medium, and static culture is carried out to obtain Lactobacillus plantarum seed liquid for final fermentation;

[0044] (4) Compounding the above-mentioned Bacillus seed liquid, Saccharomyces cerevisiae seed liquid and Lactobacillus plantarum seed liquid for the final fermentation to obtain a composite microbial agent.

[0045]In the present invention, in the step (1), the composition of the LB broth medium is preferabl...

Embodiment 1

[0061] (1) Preparation of bacillus seed liquid:

[0062] Pick 1 ring of bacillus from the slant of the preserved strains and insert it into 4 mL of LB broth medium (the medium components are peptone 0.5g, yeast powder 0.4g, sodium chloride 0.4g, pH value 7.2, sterilized Bacteria condition is 120 ℃ sterilization 15min) in the 50ml Erlenmeyer flask, at 24 ℃, 180r / min under the conditions of 180r / min shaking culture in a constant temperature shaker for 18h, to get the first-grade seed liquid, then pour the first-grade seed liquid into 40mL In a 250mL Erlenmeyer flask with LB broth medium, shake culture in a constant temperature shaker at 24°C and 180r / min for 18h, and the obtained secondary seed liquid is used as the Bacillus seed liquid for final fermentation.

[0063] (2) Preparation of Saccharomyces cerevisiae seed liquid:

[0064] Pick 1 ring of Saccharomyces cerevisiae from the slant of the preserved strains and insert it into 4mLYPD liquid medium (the medium components are...

Embodiment 2

[0071] (1) Preparation of bacillus seed liquid:

[0072] Pick 2 rings of Bacillus from the slant of the preserved strains and insert them into 5mL LB broth medium (the medium components are peptone 1g, yeast powder 0.5g, sodium chloride 0.5g, pH value 7.3, sterilized The condition is sterilized at 121°C for 20min) in a 50ml Erlenmeyer flask at 25°C and 200r / min in a constant temperature shaker for 20h to obtain the first-grade seed solution, and then pour the first-grade seed solution into 50mL LB In a 250mL Erlenmeyer flask of broth medium, shake culture in a constant temperature shaker at 25°C and 200r / min for 20h, and the obtained secondary seed liquid is used as the Bacillus seed liquid for final fermentation.

[0073] (2) Preparation of Saccharomyces cerevisiae seed liquid:

[0074] Pick 2 rings of Saccharomyces cerevisiae from the slant where the strains were preserved and insert it into 5mLYPD liquid medium (the medium components are 1g of yeast extract powder, 2g of p...

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Abstract

The invention belongs to the technical field of complex microbial inoculants, and provides a complex microbial inoculant for cottonseed meal detoxification and a preparation method and application thereof. The complex microbial inoculant comprises bacillus, saccharomyces cerevisiae and lactobacillus plantarum. The prepared complex microbial inoculant is applied to the fermentation process of the cottonseed meal, the content of free gossypol in the cottonseed meal can be effectively reduced, the content of protein and free amino acid in the cottonseed meal can be increased, when a certain amount of the complex microbial inoculant replaces soybean meal in daily ration to feed mice, the growth performance of the mice cannot be reduced, and the complex microbial inoculant has no obvious influence on biochemical indexes of livers. The complex microbial inoculant has the characteristics of simple preparation process, stable and safe product quality, low cost and the like.

Description

technical field [0001] The invention relates to the technical field of composite bacterial agents, in particular to a composite bacterial agent for detoxification of cottonseed meal. Background technique [0002] my country's protein feed resources, especially high-quality protein feed raw materials, are in serious shortage. The annual demand for grain, oil and feed resources reaches 350 million tons, of which imports account for about 1 / 3 of the demand, and the imports of soybeans and fishmeal rank first in the world. In 2018, the national cotton planting area was 33.523 million hectares, and the output reached 6.096 million tons. Cotton seeds are called cottonseed, which is an important source of oil. Cottonseed can produce 12% to 18% cottonseed oil and 45% cottonseed cake by-products through mechanical pressing or pre-pressing and extraction. The crude protein content in cottonseed meal is more than 35%, and the price is relatively low, but cottonseed meal contains endog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23L5/20C12R1/07C12R1/865C12R1/25
CPCC12N1/20C12N1/16A23L5/28
Inventor 舒文秀刘梓琦李姿茹朱凯吴慧昊臧荣鑫周瑞徐红伟
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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