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Marker and detection kit for cardia cancer screening

A technology for detecting reagents and markers, which is applied in the field of medicine and biology, can solve the problems of high mortality, lack of early warning for high-risk groups, and late treatment time for cardiac cancer patients, so as to improve the detection rate, improve the diagnosis effect, and distinguish the effect. Effect

Active Publication Date: 2021-09-28
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lack of effective biological indicators and methods for early warning and early diagnosis of high-risk groups in the prior art is the main reason for the late consultation time and high mortality of cardiac cancer patients

Method used

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  • Marker and detection kit for cardia cancer screening
  • Marker and detection kit for cardia cancer screening
  • Marker and detection kit for cardia cancer screening

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening of Serum Differential Metabolic Markers of Cardiac Cancer

[0030] 1. Experimental samples

[0031] Training set samples: Collect 60 healthy subjects (normal control group) and 60 patients with cardiac cancer (cardia cancer group) who were age- and gender-matched in Henan and Anyang Cancer Hospital according to strict screening and exclusion criteria.

[0032] The inclusion criteria for healthy subjects are: no cardiovascular, respiratory, liver, kidney, gastrointestinal, endocrine, blood, mental, or nervous system diseases and history of the above diseases, no acute or chronic diseases, no evidence of any tumor-related , no history of drug allergy, and the results of clinical laboratory tests were within the normal reference range at the time of screening.

[0033] The inclusion criteria for patients with gastric cardia cancer were as follows: patients with gastric cardia cancer confirmed by endoscopy and histopathology did not receive radiotherap...

Embodiment 2

[0060] Example 2: Evaluation of the ability of differential metabolite diagnosis to distinguish cardiac cancer patients from healthy individuals

[0061] (1) The ability of a single differential metabolite diagnosis to distinguish cardiac cancer patients from normal individuals:

[0062] Contents of Xanthurenic Acid and 2-Hydroxy-2-methylbutyric acid in the serum samples of the cardia cancer group (60 patients with cardia cancer) and normal control group (60 normal people) detected by UPLC-Q / TOF-MS in Example 1 The data were analyzed, and the receiver operating curve (ROC curve) was used to evaluate the ability of each differential metabolite to diagnose and distinguish cardiac cancer patients from normal people.

[0063] The ROC curves of Xanthurenic Acid and 2-Hydroxy-2-methylbutyric acid alone to distinguish patients with cardiac cancer and normal people are as follows figure 1 , figure 2 shown. According to the ROC curve, the area under the curve (AUC), sensitivity and...

Embodiment 3

[0077] Example 3: Application of Two Differential Metabolites in Cardiac Cancer Screening

[0078] 1. Collection of serum samples

[0079] Collect 580 healthy subjects (normal control group) and 437 cardiac cancer patients (cardia cancer group) in Henan area and Anyang Cancer Hospital according to strict screening and exclusion criteria.

[0080] The inclusion criteria for healthy subjects are: no cardiovascular, respiratory, liver, kidney, gastrointestinal, endocrine, blood, mental, or nervous system diseases and history of the above diseases, no acute or chronic diseases, no evidence of any tumor-related , no history of drug allergy, and the results of clinical laboratory tests were within the normal reference range at the time of screening.

[0081] The inclusion criteria for patients with gastric cardia cancer were as follows: patients with gastric cardia cancer confirmed by endoscopy and histopathology did not receive radiotherapy or chemotherapy.

[0082] 2. Experiment...

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Abstract

The invention belongs to the technical field of medical biology, and particularly discloses a marker and a detection kit for cardia cancer screening. The marker for screening the cardia cancer, which is provided by the invention, is at least one of Xanthurenic Acid and 2-Hydroxy-2-methylbutyric acid, and a detection reagent of the marker can be used for preparing a product for screening the cardia cancer. The invention also provides the kit for screening cardia cancer, the kit contains a detection reagent for detecting the marker, and the detection reagent is a reagent for detecting the marker in a sample through chromatography-mass spectrometry or chromatography-mass spectrometry. The cardia cancer can be effectively detected by detecting the expression levels of Xanthurenic Acid and 2-Hydroxy-2-methylbutyric acid in human serum, the detection sensitivity reaches up to 94.3%, the specificity reaches 97.0%, and the kit can be used for large-scale screening of asymptomatic crowds in a cardia cancer high-incidence area and is beneficial to screening and early discovery of asymptomatic high-risk crowds.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically discloses a marker and a detection kit for cardia cancer screening. Background technique [0002] With the development of society and the change of environment, cardia cancer has become a kind of malignant tumor that seriously threatens human life, health and safety. Cardiac cancer refers to the cancer that occurs in the anatomical part of the cardia. It is generally considered in China to be located within 2 cm below the esophagogastric junction, also known as cardia adenocarcinoma. Linzhou City, Henan Province is one of the areas with a high incidence of esophageal cancer in China. Studies have found that there is also a high incidence of cardia cancer in areas with a high incidence of esophageal cancer. This phenomenon suggests that there may be strong carcinogenic factors in the local living environment. These carcinogenic factors act on the upper gastrointestin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 赵学科王立东马琳琳李志强钟侃徐瑞华魏梦霞韩文莉雷玲玲宋昕王盼盼杨苗苗胡景峰范宗民
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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