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A kind of ssr marker for identification of citrus varieties and its application

A citrus and variety technology, applied in the field of SSR markers for identifying citrus varieties, can solve the problems affecting the upgrading and upgrading of the citrus variety renewal industry, unfavorable new citrus varieties and identification of hybrid populations, etc.

Active Publication Date: 2022-03-25
INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, many new citrus varieties have little difference in plant morphology, and can only be distinguished by fruit traits, which is not conducive to the identification of new citrus varieties and hybrid populations, and affects the renewal of citrus varieties and industrial upgrading.

Method used

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  • A kind of ssr marker for identification of citrus varieties and its application
  • A kind of ssr marker for identification of citrus varieties and its application
  • A kind of ssr marker for identification of citrus varieties and its application

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Embodiment Construction

[0021] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0022] 1. Acquisition of SSR molecular markers

[0023] The applicant resequenced the genomes of 'Qingjian' and 'Egan No. 1', developed SSR markers on a genome-wide scale, and designed primers for SSR molecular markers based on gene structure annotation results and site information.

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Abstract

The present invention relates to an SSR marker for identifying citrus varieties, which is CHR2‑2 and / or CHR8‑4, the reference sequence of CHR2‑2 is such as SEQ ID NO:1, and the reference sequence of CHR8‑4 is such as SEQ ID NO:2 shown. The present invention also provides a method for identifying citrus varieties and their hybrid offspring using the above-mentioned SSR markers. The SSR markers CHR2‑2 and CHR8‑4 of the present invention are polymorphic to common main citrus varieties. These two SSR markers have good complementarity, and after combination, they have completely different amplification characteristic peaks in almost all common main citrus varieties, can distinguish almost all common main citrus varieties, and can identify 99.23% of true progeny.

Description

technical field [0001] The invention relates to the field of citrus breeding, more particularly to an SSR marker for identifying citrus varieties and its application. Background technique [0002] my country is a big country of citrus production, and the planting area and total output of citrus rank first in the world. In recent decades, the scale of my country's citrus industry has developed rapidly. By 2019, my country's total citrus output has reached 45.845 million tons. At present, 1,753 citrus varieties have been preserved in my country, and a large number of new citrus varieties have been selected and introduced in recent years. [0003] The polyembryonic feature of citrus has seriously hindered the work of hybrid breeding, so new citrus hybrid varieties mainly select a few monoembryonic varieties as female parents, such as 'Clementine', 'Qingjian' and 'Wanggan'. As a result, many new citrus varieties have little difference in plant morphology, and can only be disti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 宋放蒋迎春吴黎明羊林鑫王志静何利刚王策马小方
Owner INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI