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Mesenchymal stem cell for treating inflammatory bowel disease and application thereof

A technology of inflammatory bowel disease and mesenchymal stem cells, applied in the direction of genetically modified cells, cells modified by introducing foreign genetic material, applications, etc., can solve problems that have not been studied and reported, and achieve strong homing ability and increase the number of , improve the effect of treatment

Active Publication Date: 2021-10-15
广州赛隽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no relevant studies and reports on how to use mesenchymal stem cells to achieve efficient and targeted therapy for inflammatory bowel disease

Method used

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  • Mesenchymal stem cell for treating inflammatory bowel disease and application thereof
  • Mesenchymal stem cell for treating inflammatory bowel disease and application thereof
  • Mesenchymal stem cell for treating inflammatory bowel disease and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Chemokine research

[0036] In this case, inflammatory bowel disease was taken as the research object, blood samples were collected, DNA was extracted, and the chemokine receptor CCR9 of patients with inflammatory bowel disease was sequenced; at the same time, tissue samples of normal people were used as controls; comparative analysis of CCR9 in Variations in inflammatory bowel disease. According to the influence of CCR9 on inflammatory bowel disease, the corresponding transfection nucleic acid is constructed. The details are as follows:

[0037] 1. Sample Collection

[0038] Take 20 mL of fresh peripheral blood from volunteers, dilute it 1:1 with 1×PBS, and separate it by density gradient centrifugation using Ficoll-Paque lymph separation medium, collect all mononuclear cells in the buffy coat, and dilute them 1:4 with sterile PBS . Centrifuge at 2000rpm for 10min and discard the supernatant. Add enough PBS to wash twice. Suspend the cells with RPMI-1640 compl...

Embodiment 2

[0091] In this example, DNFB-induced mice were used for contact hypersensitivity to verify the therapeutic effect of the transgenic mesenchymal stem cells constructed in Example 1 on inflammatory bowel disease.

[0092] In this example, male BALB / c mice aged 6-8 weeks, weighing 16-18g and raised in an SPF environment, were used for the establishment of a contact hypersensitivity model.

[0093] DNFB pre-sensitization solution: prepare a mixed solution, acetone: olive oil volume ratio 4:1, vigorously shake and mix;

[0094] Preparation of pre-sensitization mixture: use the mixture to prepare 0.5% DNFB mixture, that is, pre-sensitization mixture.

[0095] DNFB sensitization solution: use the mixed solution to prepare 0.2% DNFB mixed solution, namely the sensitized mixed solution.

[0096] Pre-sensitization: On the first day, use an electric razor to shave an area of ​​1.5×1.5 cm on the skin of the back of the mouse near the head, apply 20 μL of 0.5% DNFB pre-sensitization mixtu...

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Abstract

The invention discloses a mesenchymal stem cell for treating inflammatory bowel disease and application thereof. The mesenchymal stem cell for treating the inflammatory bowel disease is formed by transfecting nucleic acid of a chemokine receptor CCR9 to the mesenchymal stem cell. The mesenchymal stem cell for treating the inflammatory bowel disease can specifically target a lesion part of the inflammatory bowel disease, is high in homing ability, greatly improves the homing number of the mesenchymal stem cell to the lesion part, and improves the treatment effect of the inflammatory bowel disease; and a novel individualized and accurate treatment scheme and thought are provided for the treatment of the inflammatory bowel disease.

Description

technical field [0001] The present application relates to the field of drugs for treating inflammatory bowel disease, in particular to a mesenchymal stem cell for treating inflammatory bowel disease and its application. Background technique [0002] Inflammatory bowel disease (IBD) is a chronic, incurable global disease. The more developed the economy, the higher the incidence rate. It is estimated that there will be more than 1.5 million IBD patients in my country within 10 years. The main manifestations of patients are prone to recurrent abdominal pain and diarrhea, and even fistula formation, which is difficult to cure and seriously affects the work and life of patients. So far, the etiology and pathogenesis of inflammatory bowel disease have not been fully elucidated, and abnormal immune response is an important feature of this type of disease. Studies have shown that among the four major classes of chemokines, CC chemokine receptor (CCR) plays an important role in IBD;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/87C12N15/12A61K35/28A61K38/17A61P1/00
CPCC12N5/0662C12N5/0663C12N15/87C07K14/7158A61K35/28A61K38/195A61P1/00C12N2510/00Y02A50/30
Inventor 颜孙兴熊敏敏苏建华
Owner 广州赛隽生物科技有限公司
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