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Light environment regulation and control method for delaying growth of potato tissue culture seedlings

A technology of light environment and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of prolonging the growth and development process of potato tissue culture seedlings, requiring a higher technical level, and reducing hormone usage , to achieve the effects of improving energy utilization efficiency, reducing production costs, and reducing hormone usage

Active Publication Date: 2021-11-02
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production process is relatively long, the cost is high, and the technical level of the operator (hormone addition amount, etc.) is high.
[0006] So far, there is no technology to prolong the growth and development process of potato tissue culture seedlings (test-tube seedlings) by regulating the light environment to reduce hormone usage

Method used

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  • Light environment regulation and control method for delaying growth of potato tissue culture seedlings
  • Light environment regulation and control method for delaying growth of potato tissue culture seedlings
  • Light environment regulation and control method for delaying growth of potato tissue culture seedlings

Examples

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Comparison scheme
Effect test

Embodiment 1

[0049] This embodiment provides a light environment regulation method for delaying the growth of potato tissue cultured seedlings (test-tube plantlets). The light energy is all provided by the artificial light source, and does not involve direct sunlight and scattered light; when planting potato tissue culture seedlings (test-tube seedlings) in the above-mentioned plant tissue culture factory, the artificial light source is specifically:

[0050] Potato tissue cultured seedlings (test-tube plantlets) after subculture inoculation 0~48h (first tissue culture stage) and after 48h (second tissue culture stage), artificial light sources include: 45% red light, 30% of blue light, 15% of green light, 10% of the continuous spectrum of all visible light, the peak wavelength of red light is 610-660nm, the peak wavelength of blue light is 430-480nm, and the peak wavelength of green light is 540-560nm, the first During the tissue culture stage, the total light intensity of the artificial ...

Embodiment 2

[0058] This embodiment provides a light environment regulation method for delaying the growth of potato tissue cultured seedlings (test-tube plantlets). The light energy is all provided by the artificial light source, and does not involve direct sunlight and scattered light; when planting potato tissue culture seedlings (test-tube seedlings) in the above-mentioned plant tissue culture factory, the artificial light source is specifically:

[0059] Potato tissue cultured seedlings (test-tube plantlets) after subculture inoculation 0~48h (first tissue culture stage) and after 48h (second tissue culture stage), artificial light source comprises according to light quality spectral integration percentage: 40% red light, 40% of blue light, 10% of green light, 10% of the continuous spectrum of all visible light, the peak wavelength of red light is 610-660nm, the peak wavelength of blue light is 430-480nm, and the peak wavelength of green light is 540-560nm, the first During the tissue...

Embodiment 3

[0067] This embodiment provides a light environment regulation method for delaying the growth of potato tissue cultured seedlings (test-tube plantlets). The light energy is all provided by the artificial light source, and does not involve direct sunlight and scattered light; when planting potato tissue culture seedlings (test-tube seedlings) in the above-mentioned plant tissue culture factory, the artificial light source is specifically:

[0068] Potato tissue cultured seedlings (test-tube plantlets) after subculture inoculation 0~48h (first tissue culture stage) and after 48h (second tissue culture stage), artificial light sources include: 35% red light, 35% blue light, 30% green light, the peak wavelength of red light is 610-660nm, the peak wavelength of blue light is 430-480nm, the peak wavelength of green light is 540-560nm, the top of the tissue culture bottle receives The total light intensity of artificial light source is 40μmol / (m 2 s) of direct light and scattered li...

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Abstract

The invention relates to the technical field of tissue culture, in particular to a light environment regulation and control method for delaying growth of potato tissue culture seedlings. The light environment regulation and control method comprises the steps that potato tissue culture seedlings are subjected to subculture inoculation and cultured under the condition of a full-artificial light source, and according to the light quality spectrum integral percentage, the condition of the full-artificial light source comprises 0-50% of red light, 30-100% of blue light, 0-45% of green light and 0-20% of a full-visible light continuous spectrum, and the sum of the percentage is 100%; the peak wavelength of the red light is 610-660 nm, the peak wavelength of the blue light is 430-480 nm, and the peak wavelength of the green light is 540-560 nm. According to the method, a scientific and reasonable light source providing scheme is provided for prolonging the subculture period of a plant tissue culture factory and storing the potato tissue culture seedlings, the electric energy utilization rate can be increased, and the quality of the potato tissue culture seedlings is stabilized.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a light environment regulation method for delaying the growth of potato tissue culture seedlings. Background technique [0002] Tissue culture is a common asexual reproduction technique in agriculture. Place biological isolated organs, tissues or cells in the culture medium under sterile conditions, place them in a suitable environment, and obtain cells, tissues or individuals through continuous culture, so as to realize the detoxification, rapid propagation and storage of germplasm resources , Guaranteed quality, pure and off-season production. The morphological establishment and physiological and biochemical changes of seedlings in the process of plant tissue culture are regulated by many environmental factors (light, temperature, humidity, etc.). Among them, light plays an extremely important role in the growth and differentiation of plant cells, tissues, and organs. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/04
CPCA01H4/008A01G7/045Y02P60/14
Inventor 张彤李成宇王朝伟张洪杰
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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