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Prebiotic composition and preparation method thereof

A composition and prebiotic technology, applied in the field of prebiotic composition and its preparation, can solve the problems of slow onset of effect, unsatisfactory effect of stimulating growth of intestinal probiotics, ignoring the nutritional and health functions of solid drinks, etc., and achieve fast onset of effect , avoid alcohol damage to the liver, and improve the effect of intestinal flora structure

Pending Publication Date: 2021-11-02
青岛凯特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current beverages mainly focus on the taste needs of consumers, but they often have the disadvantages of unsatisfactory stimulation and slow onset of intestinal probiotics, ignoring the nutritional and health functions of solid beverages themselves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The present invention also provides a preparation method of a prebiotic composition, comprising the following steps: preheating the vegetable gum, adding the preheated enzyme solution to obtain a reaction solution 1, stirring for 5-15 minutes, and then stirring at 40-60°C React for 6-8 hours, then add 3,5-dinitrosalicylic acid, heat at 90-110°C for 2-8 minutes in water, take it out and cool it, use an ultrafiltration membrane to ultrafilter under pressure, collect the filtrate, After freeze-drying, galactomannan is obtained; a phosphate buffer solution with a pH of 5-7 is prepared into a 450-500 g / L lactose solution, and the lactose solution is added to the lactose solution to obtain a reaction solution 2, and the After heating, heat at 90-110°C for 2-8 minutes, remove and cool, add acetonitrile solution, stir evenly, filter and dry to obtain galactooligosaccharides; mix galactomannan and galactooligosaccharides Finally, the finished prebiotic composition is obtained. ...

Embodiment 1

[0030] A preparation method of a prebiotic composition, comprising the steps of:

[0031] After preheating 500ml vegetable gum at 40°C, add 400ml of β-mannan enzyme solution preheated at 40°C, stir for 5min, react at 40°C for 6h, then add 450ml of 3,5-di Nitrosalicylic acid, heated at 90°C for 2 minutes, taken out and cooled, ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 1000 under a pressure of 0.2Mpa, collected the filtrate, and freeze-dried at -30°C to obtain Galactomannan;

[0032] Prepare a phosphate buffer solution with a pH of 5 to make 450g / L 50ml lactose solution, add 25ml of Lactobacillus plantarum enzyme solution into the lactose solution, heat in a water bath at 35°C for 4h, then heat at 90°C for 2min with water separation, then take it out Cool, add 15ml of acetonitrile solution, stir evenly, filter through a 0.3-0.6μm filter membrane, and dry at 45°C to obtain galactooligosaccharides;

[0033] After mixing 85g of galactomanna...

Embodiment 2

[0035] A preparation method of a prebiotic composition, comprising the steps of:

[0036]After preheating 500ml vegetable gum at 40°C, add 500ml of β-mannan enzyme solution preheated at 40°C, stir for 5min, react at 40°C for 6h, then add 450ml of 3,5-Di Nitrosalicylic acid, heated at 90°C for 2 minutes, taken out and cooled, ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 1000 under a pressure of 0.2Mpa, collected the filtrate, and freeze-dried at -30°C to obtain Galactomannan;

[0037] Prepare a phosphate buffer solution with a pH of 6 to make 450g / L 50ml lactose solution, add 25ml of Lactobacillus plantarum enzyme solution into the lactose solution, heat it in a water bath at 35°C for 4 hours, then heat it at 90°C for 2 minutes and take it out Cool, add 15ml of acetonitrile solution, stir evenly, filter through a 0.3-0.6μm filter membrane, and dry at 45°C to obtain galactooligosaccharides;

[0038] After mixing 85g of galactomannans and 15...

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Abstract

The invention provides a prebiotic composition and a preparation method thereof, and relates to the field of prebiotic compositions. The composition contains 85-95 parts of galactomannan and 5-15 parts of galactooligosaccharide. The preparation method comprises the following steps: preheating vegetable gum, adding preheated enzyme liquid, performing reacting for 6-8 hours, adding 3, 5-dinitrosalicylic acid, heating the mixture in a waterproof manner, taking out the mixture, cooling the mixture, performing ultrafiltration by using an ultrafiltration membrane under a pressurization condition, collecting filtrate, and performing freeze-drying to obtain galactomannan; preparing a phosphate buffer into a lactose solution, adding a lactobacillus plantarum enzyme solution into the lactose solution, heating the mixture in a water bath, heating the mixture in a waterproof manner, taking out the mixture, cooling the mixture, adding an acetonitrile solution, uniformly stirring the mixture, filtering the mixture, and drying the mixture to obtain galactooligosaccharide; and mixing the galactomannan and the galactooligosaccharide to obtain a finished product. The prebiotic composition has higher fermentability and hydrolyzability, and the generated short-chain fatty acid can better influence the growth and colonization of probiotics.

Description

technical field [0001] The invention relates to the field of prebiotic compositions, in particular to a prebiotic composition and a preparation method thereof. Background technique [0002] The intestine is the largest immune organ of the human body, and also the largest detoxification organ of the human body. It controls more than 70% of the immune function of the human body and becomes a natural barrier to maintain human health. As the saying goes: "Disease enters through the mouth", most germs are eaten from the mouth, and the main way for bacteria to enter the human body is the intestines. As you can imagine, gut health depends on gut activity. [0003] Prebiotics are non-digestible food ingredients that beneficially affect the host by selectively stimulating the growth and activity of one or a small number of bacteria in the colony to improve the health of the host. Successful prebiotics should pass through the upper gastrointestinal tract, most of which are not dige...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/125A23L33/21C12P19/00C12P19/04C12P19/14
CPCA23L33/125A23L33/21C12P19/14C12P19/04C12P19/00A23V2002/00A23V2200/3202A23V2250/28A23V2250/505
Inventor 常庆秀
Owner 青岛凯特生物科技有限公司
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