Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SNP (Single Nucleotide Polymorphism) molecular marker located on 12 # chromosome of pig and related to piglet malformation number and application of SNP molecular marker

A molecular marker and chromosome technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of inability to fundamentally solve the genetic defects of pigs, restrict the rapid transmission of excellent genes, and eliminate individuals. Achieve the effect of increasing core competitiveness, reducing the number of deformed piglets, and ensuring corporate profits

Pending Publication Date: 2021-11-02
SOUTH CHINA AGRI UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, in the actual breeding production, the breeding pigs with genetic defects will not be kept for breeding, and even the individuals in their families will be eliminated, which is very likely to limit the rapid transmission of excellent genes in the population
Genetic defects are a complex class

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP (Single Nucleotide Polymorphism) molecular marker located on 12 # chromosome of pig and related to piglet malformation number and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker located on 12 # chromosome of pig and related to piglet malformation number and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker located on 12 # chromosome of pig and related to piglet malformation number and application of SNP molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) The extraction method of pure Duroc pig ear-like tissue DNA was extracted from the whole genome DNA according to the marked phenol-chloroform method. The DNA quality and concentration of the pure Duroc population were tested with a Nanodrop-ND1000 spectrophotometer. The ratio of A260 / 280 is 1.8-2.0, and the ratio of A260 / 230 is 1.7-1.9. Finally, the qualified DNA samples were uniformly diluted to 50 ng / μl. Then mix 6 μL of the extracted DNA sample to be tested with 2 μL Loading Buffer, load the sample on agarose gel with a mass-volume ratio of 1%, electrophoresis at 150V for 25 min, and perform electrophoresis under UV spectrophotometer and gel imaging equipment Observe and take pictures to observe the integrity of DNA. After passing the DNA integrity test, the genotype test was performed.

[0035] (2) Detection of 50K SNP genotype of the whole pig genome: DNA samples were sent to Neogen Biotechnology (Shanghai) Co., Ltd., and the genotype of the pig whole genome...

Embodiment 2

[0042] Example 2 Detection of SNP molecular markers

[0043] (1) The target fragment containing the SNP site significantly related to the number of deformities in offspring of Duroc pigs is a 224bp nucleotide sequence in chromosome 12, and the upstream and downstream primers for sequence amplification are primer-F and primer-R , its nucleic acid sequence is as follows:

[0044] Upstream primer primer-F: 5'-TGCCTGGCCTCTCACTGTCACAC-3';

[0045] Downstream primer primer-R: 5'-ACTCCTCTTTGTCCCCGGCTT-3';

[0046] (2) PCR amplification system and condition setting

[0047] Configure a 10 μL system, including 1.0 μL DNA sample, 0.3 μL upstream primer, 0.3 μL downstream primer, 5.0 μL PCR StarMix, ddH 2 O 3.4 μL, PCR conditions were pre-denaturation at 94°C for 2 min, denaturation at 94°C for 30 s, annealing at 64°C for 30 s, extension at 72°C for 15 s, a total of 35 cycles, and a final extension at 72°C for 5 min.

[0048] (3) DNA sequence sequencing identification: Sequence seque...

Embodiment 3

[0051] Example 3 SNP site g.121A>G effect analysis of molecular markers

[0052] According to Table 1, figure 2 It can be seen that the molecular marker SNP site g.121A>G (the 121st nucleotide in SEQ NO.1) has a very significant correlation with the number of piglet deformities. The number of deformed piglets of GG and AG type individuals was less than that of AA type individuals, and the number of deformed piglets of GG type individuals was 1.22 less than that of AA type individuals. Therefore, keeping the breeding pigs of GG and AG genotypes in breeding to gradually increase the frequency of the allele G at this site can significantly reduce the number of piglet deformities and bring greater economic benefits to the enterprise.

[0053] The present invention detects the 121st base mutation site in the sequence of SEQ ID NO: 1, and preliminarily conducts a correlation analysis between its genotype and the occurrence of piglet deformities, and provides a new molecular marker...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker located on a 12 # chromosome of a pig and related to a piglet malformation number and the application of the SNP molecular marker. The site of the SNP molecular marker corresponds to A > G mutation at the 13982775th site on the 12 # chromosome of a reference sequence of an international pig genome version 11.1. The SNP molecular marker is obtained through whole genome association analysis, and the SNP molecular marker is significantly related to the number of malformation of a single piglet. The invention also provides a primer pair for recognizing the molecular marker, an efficient and accurate molecular marker assisted breeding technology can be established by using the molecular marker and the primer pair, and the malformation number of piglets can be greatly reduced when the molecular marker assisted breeding technology is applied to genetic improvement of boars.

Description

technical field [0001] The invention relates to a SNP molecular marker which is located on the No. 12 pig chromosome and is related to the deformity number of piglets and its application. Background technique [0002] my country is the largest pork producer in the world, and the number of live pigs for slaughter accounts for more than half of the total number of live pigs for slaughter in the world. The output value and economic benefits created by the pig industry have a very important impact on the development of the national economy. However, malformed newborn piglets (comprising genetic defects such as scrotum hernia, single testicle, splayed feet, and lock anus) often bring huge economic losses to the pig industry. On the one hand, about 1.8 million piglets die each year due to genetic defects, which brings economic losses of up to hundreds of millions to my country's pig industry every year. On the other hand, in actual breeding production, breeding pigs with genetic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2535/122
Inventor 吴珍芳杨杰徐此能丁荣荣郑恩琴全建平蔡更元黄思秀顾婷杨化强
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com