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Plant source biocontrol bacterial preparation for preventing and treating rubber colletotrichum gloeosporioides

A bio-control bacteria, plant-derived technology, applied in the direction of plant growth regulators, fungicides, biocides, etc., can solve the problems of rubber tree leaves falling off, death, loss of rubber industry development, etc., and achieve good inhibitory effect.

Active Publication Date: 2021-11-05
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rubber tree anthracnose is caused by the infection of Glucosporum anthracnose. In Hainan and Yunnan, the leaves of countless rubber trees fall off, and even the whole plant dies, causing serious losses to the development of the local rubber industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The water extract of LMZ was prepared by water extraction method by taking LMZ; the ethanol extract of citrus peel was obtained by alcohol extraction method by taking citrus peel.

[0016] According to parts by weight, get 40 parts of crushed corn cobs, 50 parts of bean dregs, 10 parts of Cortex Phellodendri, 10 parts of Angelica dahurica dregs, 10 parts of reed rhizome dregs and appropriate amount of water to make fermentation substrate, and control the water content of fermentation substrate to 60-70% section, then inoculated with EM bacteria and fermented for 12 days, then filtered to obtain EM bacteria fermentation broth.

[0017] Mix the EM bacteria fermentation liquid, the water extract of Liangmianzhen and the ethanol extract of tangerine peel, so that the content of the water extract of Liangmianzhen is 0.1wt%, the content of the ethanol extract of citrus peel is 0.3wt%, and the total viable bacteria of EM bacteria Number 5×10 7 cfu / ml, vested.

Embodiment 2

[0021] The water extract of LMZ was prepared by water extraction method by taking LMZ; the ethanol extract of citrus peel was obtained by alcohol extraction method by taking citrus peel.

[0022] According to parts by weight, get 50 parts of crushed corncobs, 40 parts of bean dregs, 5 parts of Cortex Phellodendri dregs, 10 parts of Angelica dahurica dregs, 5 parts of reed rhizome dregs and an appropriate amount of water to make a fermentation substrate, and control the water content of the fermentation substrate to 60-70% section, then inoculated with EM bacteria and fermented for 10 days, then filtered to obtain EM bacteria fermentation broth.

[0023] Mix the EM bacteria fermentation liquid, the water extract of Liangmianzhen and the ethanol extract of tangerine peel, so that the content of the water extract of Liangmianzhen is 0.3wt%, the content of the ethanol extract of citrus peel is 0.1wt%, and the total viable bacteria of EM bacteria Number 8×10 7 cfu / ml, vested.

Embodiment 3

[0027] The water extract of LMZ was prepared by water extraction method by taking LMZ; the ethanol extract of citrus peel was obtained by alcohol extraction method by taking citrus peel.

[0028] In terms of parts by weight, get 40 parts of crushed corncobs, 50 parts of bean dregs, 10 parts of Cortex Phellodendron dregs, 5 parts of Angelica dahurica dregs, 5 parts of reed rhizome dregs and an appropriate amount of water to make a fermentation substrate, and control the water content of the fermentation substrate to 60-70% section, then inoculated with EM bacteria and fermented for 9 days, then filtered to obtain EM bacteria fermentation broth.

[0029] Mix the EM bacteria fermentation liquid, the water extract of Liangmianzhen and the ethanol extract of tangerine peel, so that the content of the water extract of Liangmianzhen is 0.12wt%, the content of the ethanol extract of citrus peel is 0.2wt%, and the total viable bacteria of EM bacteria Number 6×10 7 cfu / ml, vested.

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Abstract

The invention provides a plant source biocontrol bacterial preparation for preventing and treating rubber colletotrichum gloeosporioides, wherein the plant source biocontrol bacterial preparation is composed of EM bacterium fermentation liquor, a zanthoxylum nitidum aqueous extract and a citrus peel ethanol extract, the content of the zanthoxylum nitidum aqueous extract is 0.1-0.3 wt%, the content of the citrus peel ethanol extract is 0.1-0.3 wt%, and the total viable count of EM bacteria is greater than or equal to 5*10<7> cfu / ml. Effective components of the plant source biocontrol bacterial preparation are composed of the EM bacteria and fermentation metabolites thereof, the zanthoxylum nitidum aqueous extract and the citrus peel ethanol extract which belong to active components with different action mechanisms, have a better inhibition effect on rubber colletotrichum gloeosporioides under the synergistic effect, and can effectively prevent and treat rubber colletotrichum gloeosporioides; and all the components belong to green, environment-friendly and non-toxic active components with dual biological regulation functions of pesticide effect and fertilizer effect.

Description

technical field [0001] The invention relates to a plant-derived biological control bacteria preparation, in particular to a plant-derived biological control bacteria preparation for preventing and treating rubber anthracnose bacteria. Background technique [0002] Anthracnose can infect a variety of plants and cause anthracnose. This kind of disease is widely distributed and seriously harmful. It is one of the key research objects of plant pathologists. Rubber tree anthracnose is the most serious one. Rubber tree anthracnose is caused by the infection of Glucosporum anthracnose. In Hainan and Yunnan, the leaves of countless rubber trees fall off, and even the whole plant dies, causing serious losses to the development of the local rubber industry. [0003] Anthracnose mainly overwinters in diseased branches in the form of mycelium, and produces conidia under suitable conditions as the primary source of infection. Rubber tree seedlings, young trees until mature rubber trees ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/36A01N63/00A01P3/00
CPCA01N65/00A01N65/36A01N63/00
Inventor 刘文波秦春秀缪卫国陈代朋
Owner HAINAN UNIVERSITY
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