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Purification method and application of soybean cyst nematode new race X12

A technology of soybean cyst nematode and purification method, which is applied in the field of purification of a new soybean cyst nematode race X12, which can solve the problems of soybean economic loss and achieve the effects of stable offspring and high homozygosity of the population

Inactive Publication Date: 2021-11-09
HENAN ACAD OF AGRI SCI
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  • Claims
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Problems solved by technology

However, soybean cyst nematode (Soybean Cyst Nematode, SCN) is widely distributed in the main soybean producing areas of the world, causing serious economic losses to soybean production.

Method used

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  • Purification method and application of soybean cyst nematode new race X12
  • Purification method and application of soybean cyst nematode new race X12
  • Purification method and application of soybean cyst nematode new race X12

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Embodiment 1

[0025] A method for purifying soybean cyst nematode new race X12, comprising the following steps:

[0026] (1) Collect the diseased soil infected by soybean cyst nematode X12, uniformly mix the diseased soil and sterile fine sand at a volume ratio of 3:0.5-1.0, prepare the test soil, and pack it in soybean cyst nematode-resistant In a disposable plastic test cup for identification;

[0027] The collection method of the diseased soil is as follows: according to the reported investigation and research results, the soil between 2cm-6cm below the soil surface is collected from the soybean field infected by soybean cyst nematode X12, and the soil is used for subsequent use; the soybean cyst nematode has 9 pairs of chromosomes;

[0028] (2) Transplant 3-5 days of germination and the unearthed cotyledon stage of soybean excellent resistant Xingxian Huipi branch (number: ZDD2315) into experimental cups with test diseased soil, and transplant 1 plant in each experimental cup. grown in...

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Abstract

The invention provides a purification method and application of a soybean cyst nematode new race X12. The purification method comprises the steps that a soybean cyst nematode group identified as a soybean cyst nematode X12 physiological race is subjected to continuous single-cyst propagation on domestic excellent resistance-source Chrysochelon in Xing County (numbering: ZDD2315) for 4 generations by adopting a single-cyst propagation method, and is continuously purified and propagated on the Chrysochelon in Xing County for 3-4; and the first generation is recovered by using the susceptible variety Lee to realize population purification and propagation of the cysts. The purification method can be used for separating and purifying the X12 race with superstrong pathogenicity from soybean cyst nematode groups mixed in nature, the purified X12 microspecies can be continuously used for genome sequencing research, soybean cyst nematode disease-causing gene research, soybean resistance resource screening, soybean cyst nematode-resistant gene positioning and phenotype identification screening research in the soybean disease-resistant variety cultivation process in multiple generations, and has important application value in related research of soybean cyst nematode.

Description

technical field [0001] The invention relates to a purification method and application of a new soybean cyst nematode race X12, belonging to the field of biotechnology. Background technique [0002] Soybean is an important source of people's intake of vegetable protein and vegetable oil, so soybean plays a pivotal role in agricultural production. However, soybean cyst nematode (Soybean Cyst Nematode, SCN) is widely distributed in the world's major soybean production areas, causing serious economic losses to soybean production. The Huanghuai area is one of the two major soybean producing areas in my country. Preliminary research results show that soybean cyst nematodes are common in this area, and No. 2 is the dominant physiological race, followed by No. 5 physiological race. During the investigation and research, a new race X12 of soybean cyst nematode with super pathogenicity was also detected. It belongs to a new race of soybean cyst nematode with super pathogenicity. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/0336
Inventor 练云李海朝王金社王仕伟雷晨芳武永康李金英魏荷卢为国
Owner HENAN ACAD OF AGRI SCI
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