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Pure white jiji mushroom strain and its molecular markers, specific primer pairs and applications

A technology of molecular markers and shimeji mushrooms, which is applied in the direction of fungi, biochemical equipment and methods, microorganisms, etc., can solve the problems of easy degeneration and mutation of strains, and achieve the effect of long preservation period, high yield per unit area, and less occurrence

Inactive Publication Date: 2018-12-21
SHANGHAI FINC BIO TECH INC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention also provides a breeding method for stabilizing the pure white mushroom strain, which is used to solve the current targeted breeding method for the problem that the strain is prone to degenerate variation in the subculture process

Method used

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  • Pure white jiji mushroom strain and its molecular markers, specific primer pairs and applications
  • Pure white jiji mushroom strain and its molecular markers, specific primer pairs and applications
  • Pure white jiji mushroom strain and its molecular markers, specific primer pairs and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Finc-W-5 obtained by selfing of polyspores

[0066] The pure Shimeji mushroom strain Finc-W-5 was obtained by the method of polyspore self-breeding.

[0067] Specific steps are as follows:

[0068] 1) The parent strain H-W purchased in the market;

[0069] 2) Preparation of mother seed culture medium, original seed culture medium and cultivated seed culture medium: the formula of each culture medium has been given, so I will not repeat it here;

[0070] 3) Separation of basidiospores: Place the parent strain H-W in a petri dish, and collect the spores that fall in the petri dish after 24 hours, dilute it in sterile water to an appropriate concentration, and spread it on a PDA medium plate. Incubate at 23°C for 10 days. After the spores germinate, use an inoculation loop to inoculate the PDA oblique tubes. These test tubes were cultured at 23°C for 12 days, and then the hyphae were separated from the test tubes, and the formation of lock-like unions was observed under ...

Embodiment 2

[0074] Example 2 Antagonism Experiment

[0075] The Finc-W-5 and the parent strain H-W of the present invention are test materials, and the medium used is a PDA medium.

[0076] Finc-W-5 and the parent strain H-W were inoculated and cultured on a plate medium. The inoculation points were 2.5cm apart and incubated at 23°C for 18-20 days. Please refer to Table 1 for the results of this test.

[0077] Table 1 Antagonistic test among different pure Shimeji mushroom strains

[0078] Variety

[0079] Such as Figure 1-2 As shown, there is an obvious line of antagonism between the junctions of the confronting culture colonies, indicating that Finc-W-5 is a pure white Shimeji mushroom strain different from the parent strain H-W.

Embodiment 3

[0080] Example 3 Cultivation experiment

[0081] The Finc-W-5 of the present invention and the parent strain HW are separately cultured and cultivated. 4 bacterial ages are set from 70 days to 85 days, and each additional 5 days is a bacterial age, and each bacterial age is 96 bottles, and then small batches are cultivated The results are summarized and compared, and the advantages and characteristics of the strain Finc-W-5 of the present invention are summarized.

[0082] The culture medium of this embodiment participates in the culture medium of the cultivated species, and will not be repeated.

[0083] Cultivation bottle volume: 850cc, bottle mouth diameter 58mm.

[0084] Cultivation: The temperature is 18-23℃, the relative humidity of the air is 65-75%, and the carbon dioxide concentration is 2000~3500ppm.

[0085] Cultivation: The fruiting temperature is set to 13℃~15℃, the relative humidity of the air is 93%~100%, the carbon dioxide concentration is 1000~2000ppm, the light intens...

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Abstract

The invention belongs to the technical field of edible fungi, and in particular relates to a pure white mushroom strain, a molecular marker, a pair of specific primers and an application thereof. The name of the pure white Hypsizygus strain provided by the present invention is Finc-W-5, which belongs to Hypsizygus marmoreus, and is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, address: 1 Beichen West Road, Chaoyang District, Beijing No. 3 Courtyard, the preservation number is CGMCC No.12520, and the preservation date is June 30, 2016. According to the technical scheme provided by the present invention, the obtained new strains have high homozygosity, stable vitality, are not easy to degenerate and mutate, have a short cultivation period, relatively concentrated fruiting time, high per unit yield, beautiful flower shape, and long fresh-keeping period, and are very suitable for large-scale Area promotion planting, the market prospect is broad.

Description

Technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to a pure Shimeji mushroom strain and its molecular marker, specific primer pair and application. Background technique [0002] Hypsizygus marmoreus (Latin name Hypsizygus marmoreus), belongs to the Basidiomycotina, Layer Fungi, Agaricus, Tricholomaceae, Ligularia, and Yumushroom. It is also called because of its unique crab flavor. It is a crab mushroom. At present, there are two varieties of light gray and pure white cultivated. The white varieties are also called "White Jade Mushroom" and "Yulong Mushroom", which are very popular in the market. Most of the pure white Jade mushrooms are cultivated in factories. [0003] The first artificial cultivation began in Japan around 1978. Its taste is delicious, crispy and nutritious. Its flavor is fresher than oyster mushrooms, its meat is thicker than oyster mushrooms, its texture is tougher than shiitake mushrooms, and its ta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N15/11C12Q1/6895C12Q1/04A23L31/00C12R1/645
CPCC12N1/14C12Q1/6895C12Q2600/156C12N1/145C12R2001/645
Inventor 韩成玲钱仙云张文宏吴慧敏
Owner SHANGHAI FINC BIO TECH INC
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