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Extraction method and application of Boletus laetissimus Hongo crude polysaccharide

An extraction method and a crude polysaccharide technology are applied in the field of extraction of the crude polysaccharide of Boletus orange, which can solve the problems of low extraction efficiency, easy inactivation of compound enzymes, and great influence of enzyme temperature, so as to improve the yield and improve the extraction efficiency. Effect

Pending Publication Date: 2021-06-04
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction of crude polysaccharides from boletus is mostly based on compound enzyme enzymatic hydrolysis, but the enzyme is greatly affected by temperature and pH control, it is not easy to control during the extraction process, and the compound enzyme is easily inactivated, resulting in low extraction efficiency and obtaining The amount of crude polysaccharides is small, which is not conducive to the study of the antioxidant activity of boletus orange polysaccharides

Method used

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  • Extraction method and application of Boletus laetissimus Hongo crude polysaccharide
  • Extraction method and application of Boletus laetissimus Hongo crude polysaccharide
  • Extraction method and application of Boletus laetissimus Hongo crude polysaccharide

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Effect test

Embodiment 1

[0036] A kind of extraction method of orange boletus crude polysaccharide, comprises the steps:

[0037] 1) drying and pulverizing: drying and pulverizing the orange boletus, and passing through a 40-mesh sieve to obtain powder;

[0038] 2) Soaking: Add 95% ethanol to the powder obtained in step 1) for soaking, quickly filter with filter paper at room temperature, and collect the filter residue; wherein, the volume ratio of powder to ethanol is 1:15;

[0039] 3) Water extraction: add distilled water to the filter residue obtained in step 2), extract in a water bath at 80° C. for 2.5 hours, filter, collect the filtrate, carry out water extraction on the filter residue again, repeat twice, and combine the filtrate to obtain a water extract; wherein, The volume ratio of filter residue to distilled water is 1:15;

[0040] 4) Concentration: Concentrate the water extract obtained in step 3) under reduced pressure at 0.09 MPa and 45° C. for 1 hour to obtain a primary concentrate;

...

Embodiment 2

[0044] A kind of extraction method of orange boletus crude polysaccharide, comprises the steps:

[0045] 1) drying and pulverizing: drying and pulverizing the orange boletus, and passing through a 40-mesh sieve to obtain powder;

[0046] 2) Soaking: Add 95% ethanol to the powder obtained in step 1) for soaking, quickly filter with filter paper at room temperature, and collect the filter residue; wherein, the volume ratio of powder to ethanol is 1:25;

[0047] 3) Water extraction: add distilled water to the filter residue obtained in step 2), extract in a water bath at 90° C. for 3.5 hours, filter, collect the filtrate, carry out water extraction on the filter residue again, repeat 3 times, and combine the filtrate to obtain a water extract; wherein, The volume ratio of filter residue to distilled water is 1:25;

[0048] 4) Concentration: Concentrate the water extract obtained in step 3) under reduced pressure at 0.095 MPa and 45°C for 2 hours to obtain a primary concentrate; ...

Embodiment 3

[0052] A method for extracting crude polysaccharides from boletus orange, comprising the steps of:

[0053] 1) drying and pulverizing: drying and pulverizing the orange boletus, and passing through a 40-mesh sieve to obtain powder;

[0054] 2) Soaking: Add 95% ethanol to the powder obtained in step 1) for soaking, quickly filter with filter paper at room temperature, and collect the filter residue; wherein, the volume ratio of the powder to the organic solvent is 1:20;

[0055] 3) Water extraction: add distilled water to the filter residue obtained in step 2), extract in a water bath at 85° C. for 3 hours, filter, collect the filtrate, carry out water extraction on the filter residue again, repeat 3 times, and combine the filtrate to obtain a water extract; wherein, the filter residue The volume ratio with distilled water is 1:20;

[0056] 4) Concentration: Concentrate the water extract obtained in step 3) under reduced pressure at 0.10 MPa and 45°C for 1.5 hours to obtain a pr...

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Abstract

The invention discloses a Boletus laetissimus Hongo crude polysaccharide extraction method which comprises the following steps: drying, crushing and sieving Boletus laetissimus Hongo to obtain powder; adding an organic solvent into the obtained powder for soaking, filtering, and collecting filter residues; adding distilled water into the obtained filter residues, carrying out water bath extraction at 80-90 DEG C for 2.5-3.5 hours, filtering the mixture, collecting filtrate, carrying out water extraction on the filter residues again, repeating the steps for 2-3 times, and combining the filtrate to obtain a water extract; then concentrating the obtained water extract under reduced pressure to obtain a primary concentrated solution; removing impurity protein in the concentrated solution, repeating the step for 4-6 times, and dialyzing and concentrating the liquid to obtain a secondary concentrated solution; and adding an organic solvent into the secondary concentrated solution, performing standing treatment at 4 DEG C for 24-48 hours, centrifuging the liquid, collecting the precipitate, and freeze-drying the precipitate to obtain the crude polysaccharide.

Description

technical field [0001] The invention relates to the technical field of biological extraction, and more specifically relates to an extraction method and application of a boletus aurantiacus crude polysaccharide with antioxidant activity. Background technique [0002] Orange boletus [Crocinoboletus rufoaureus (Massee) N.K.Zeng, Zhu L.Yang & G.Wu] is a fungus of the genus Crocinoboletus in the family Boletaceae (Boletaceae), also known as the golden red boletus, the main feature is the cover The surface and stalk surface are golden red, the bacterial flesh quickly turns blue-green after being injured, and the base hyphae are golden yellow. This species is mainly distributed in Hainan, Fujian and other regions in China. Its fruiting body contains a special pigment component - boletocrocin (boletocrocin). [0003] At present, the extraction of crude polysaccharides from boletus is mostly based on compound enzyme enzymatic hydrolysis, but the enzyme is greatly affected by temper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 曾念开王勇柴慧张玉卓谢惠菁张絮徐畅
Owner HAINAN MEDICAL COLLEGE
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