Full-automatic nucleic acid extraction, amplification and detection integrated reaction tube and column type detection card

Abstract

The invention relates to the field of in-vitro-diagnosis nucleic acid detection and provides a full-automatic nucleic acid extraction, amplification and detection integrated reaction tube and a column type detection card. The integrated reaction tube sequentially comprises a right-angle circular truncated cone section, a cylindrical section and a right-angle conical section along a direction from an opening part to the bottom, the right-angle circular truncated cone section forms a cracking area, the cylindrical section forms a washing area, the right-angle conical section forms an elution amplification area, and the right-angle side of the right-angle circular truncated cone section and the right-angle side of the right-angle conical section are located at the same side and are flush with the side portion of the cylindrical section. The column type detection card is the integrated reaction tube filled with a lysis solution, a cleaning solution and a reaction solution, wherein the solutions are separated by phase-change separators. The reaction tube not only can complete a whole extraction and amplification detection process in one tube, but also has the characteristics of simple structure, convenience in production, simple magnet movement structure of matched detection equipment and the like.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic nucleic acid detection, in particular to a reaction tube integrating automatic nucleic acid extraction, amplification and detection, and a column detection card using the reaction tube. Background technique [0002] From nucleic acid extraction to amplification detection, there are steps such as lysis, washing, elution, and amplification. The current mainstream technology must be carried out step by step in a standard laboratory. The whole process is easy to pollute the sample nucleic acid, and the operation is difficult. High personnel requirements are not conducive to the promotion of nucleic acid detection technology. [0003] The Chinese invention patent application with the publication number CN111876468A discloses a fully automatic nucleic acid detection test tube, which includes: a main body, an annular pipe groove, a tapered pipe body, and a flip cover; The body is connected through the...

AI Technical Summary

Problems solved by technology

But still there is following defect: 1, the structure of detection test tube is complicated, is unfavorable for production

Since the detection test tube includes an annular tube groove, resulting in a complex structure, it is not conducive to the production of the detection test tube, and it is also not conducive to the packaging of the three liquids

2. The direction of movement of the external magnets is inconsistent, which will increase the complexity of the supporting detection equipment

The movement of the magnetic beads is driven by the external magnet, so the external magnet needs to be able to move up and down, and it must also be able to make a circular motion around the annular pipe groove, which makes the structure of the matching detection equipment complicated.

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