Urine oxalic acid content detection kit and detection method

Abstract

The invention discloses a urine oxalic acid content detection kit and a detection method. The kit comprises a reagent R1 and a reagent R2. The reagent R1 is prepared from the following components: citric acid, DMAB and MBTH, the concentration of the citric acid in the reagent R1 is 0.01 to 1M, and the concentration of the DMAB in the reagent R1 is 0.1 to 0.5 mM; the concentration of the MBTH in the reagent R1 is 0.01 to 0.5 mM; the reagent R2 comprises the following components: a buffer solution with the pH value of 5.6, oxalate oxidase and peroxidase; the concentration of the buffer solution in the R2 reagent is 0.1 M, the concentration of the oxalate oxidase in the R2 reagent is greater than 50 U / L, and the concentration of the peroxidase in the R2 reagent is greater than 5000 U / L; and the R2 reagent is in a dry powder state and is redissolved by water when being used. The kit disclosed by the invention has a relatively high detection limit, can be used for detecting the content of oxalic acid in urine, has relatively high detection sensitivity, and has the advantages of simplicity and rapidness in operation.

Description

technical field

[0001] The invention relates to the technical field of detection of oxalic acid content in urine, in particular to a urine oxalic acid detection kit and a detection method. Background technique

[0002] The scientific name of oxalic acid is oxalic acid, and its molecular weight is 90.2. It can enter the body directly through diet and is finally metabolized by the liver.

[0003] There are two sources of oxalic acid in the human body: ① The exogenous oxalic acid absorbed by the intestine is obtained from food, accounting for about 10% of the total oxalic acid. ② The endogenous oxalic acid caused by the decomposition of protein and carbohydrate in the body and some drugs accounts for about 90%. Total excretion of oxalate includes endogenous and dietary oxalate that accumulates after microbial degradation. Nearly half of dietary oxalate is decomposed by bacteria, which have the ability to enzymatically decompose oxalate within a certain pH range. Some patient...

Examples

Embodiment 1

[0049] Urine oxalate content detection kit, including R1 reagent and R2 reagent;

[0050] The R1 reagent comprises the following components:

[0051] Citric acid, DMAB and MBTH, the concentration of the citric acid in the R1 reagent is 0.07M, the concentration of the DMAB in the R1 reagent is 0.18mM; the concentration of the MBTH in the R1 reagent is 0.09mM;

[0052] R2 reagents include the following components:

[0053] The buffer solution, oxalate oxidase and peroxidase that pH value is 5; The concentration of described buffer solution in R2 reagent is 0.06M, and the concentration of described oxalate oxidase in R2 reagent is 100U / L, and described process The concentration of oxidase in the R2 reagent is 5000U / L; the buffer is a citrate buffer, and the citrate buffer has 0.06M citric acid and 0.06M sodium citrate in a volume ratio of 41: 59 mixed to obtain;

[0054] The R2 reagent is in a dry powder state and is reconstituted with water when used.

[0055] The detection ...

Embodiment 2

[0058] This embodiment is based on embodiment 1, and the difference with embodiment 1 is that the concentration of each component is different, specifically:

[0059] Urine oxalate content detection kit, including R1 reagent and R2 reagent;

[0060] The R1 reagent comprises the following components:

[0061] Citric acid, DMAB and MBTH, the concentration of the citric acid in the R1 reagent is 0.2M, the concentration of the DMAB in the R1 reagent is 0.29mM; the concentration of the MBTH in the R1 reagent is 0.2mM;

[0062] R2 reagents include the following components:

[0063] The buffer solution, oxalate oxidase and peroxidase that pH value is 5.8; The concentration of described buffer solution in R2 reagent is 0.2M, and the concentration of described oxalate oxidase in R2 reagent is 300U / L, and described process The concentration of oxidase in the R2 reagent is 10000U / L; The buffer is a citrate buffer, and the citrate buffer has 0.2M citric acid and 0.2M sodium citrate in a...

Embodiment 3

[0066] This embodiment is based on embodiment 1, and the difference with embodiment 1 is that the concentration of each component is different, specifically:

[0067] Urine oxalate content detection kit, including R1 reagent and R2 reagent;

[0068] The R1 reagent comprises the following components:

[0069] Citric acid, DMAB and MBTH, the concentration of the citric acid in the R1 reagent is 0.15M, the concentration of the DMAB in the R1 reagent is 0.2mM; the concentration of the MBTH in the R1 reagent is 0.15mM;

[0070] R2 reagents include the following components:

[0071] The buffer solution, oxalate oxidase and peroxidase that pH value is 5; The concentration of described buffer solution in R2 reagent is 0.13M, and the concentration of described oxalate oxidase in R2 reagent is 200U / L, and described process The concentration of oxidase in the R2 reagent is 8000U / L; the buffer is a citrate buffer, and the citrate buffer has 0.13M citric acid and 0.13M sodium citrate in ...

technical field

[0001] The invention relates to the technical field of detection of oxalic acid content in urine, in particular to a urine oxalic acid detection kit and a detection method. Background technique

[0002] The scientific name of oxalic acid is oxalic acid, and its molecular weight is 90.2. It can enter the body directly through diet and is finally metabolized by the liver.

[0003] There are two sources of oxalic acid in the human body: ① The exogenous oxalic acid absorbed by the intestine is obtained from food, accounting for about 10% of the total oxalic acid. ② The endogenous oxalic acid caused by the decomposition of protein and carbohydrate in the body and some drugs accounts for about 90%. Total excretion of oxalate includes endogenous and dietary oxalate that accumulates after microbial degradation. Nearly half of dietary oxalate is decomposed by bacteria, which have the ability to enzymatically decompose oxalate within a certain pH range. Some patient...

Examples

Embodiment 1

[0049] Urine oxalate content detection kit, including R1 reagent and R2 reagent;

[0050] The R1 reagent comprises the following components:

[0051] Citric acid, DMAB and MBTH, the concentration of the citric acid in the R1 reagent is 0.07M, the concentration of the DMAB in the R1 reagent is 0.18mM; the concentration of the MBTH in the R1 reagent is 0.09mM;

[0052] R2 reagents include the following components:

[0053] The buffer solution, oxalate oxidase and peroxidase that pH value is 5; The concentration of described buffer solution in R2 reagent is 0.06M, and the concentration of described oxalate oxidase in R2 reagent is 100U / L, and described process The concentration of oxidase in the R2 reagent is 5000U / L; the buffer is a citrate buffer, and the citrate buffer has 0.06M citric acid and 0.06M sodium citrate in a volume ratio of 41: 59 mixed to obtain;

[0054] The R2 reagent is in a dry powder state and is reconstituted with water when used.

[0055] The detection ...

Embodiment 2

[0058] This embodiment is based on embodiment 1, and the difference with embodiment 1 is that the concentration of each component is different, specifically:

[0059] Urine oxalate content detection kit, including R1 reagent and R2 reagent;

[0060] The R1 reagent comprises the following components:

[0061] Citric acid, DMAB and MBTH, the concentration of the citric acid in the R1 reagent is 0.2M, the concentration of the DMAB in the R1 reagent is 0.29mM; the concentration of the MBTH in the R1 reagent is 0.2mM;

[0062] R2 reagents include the following components:

[0063] The buffer solution, oxalate oxidase and peroxidase that pH value is 5.8; The concentration of described buffer solution in R2 reagent is 0.2M, and the concentration of described oxalate oxidase in R2 reagent is 300U / L, and described process The concentration of oxidase in the R2 reagent is 10000U / L; The buffer is a citrate buffer, and the citrate buffer has 0.2M citric acid and 0.2M sodium citrate in a...

Embodiment 3

[0066] This embodiment is based on embodiment 1, and the difference with embodiment 1 is that the concentration of each component is different, specifically:

[0067] Urine oxalate content detection kit, including R1 reagent and R2 reagent;

[0068] The R1 reagent comprises the following components:

[0069] Citric acid, DMAB and MBTH, the concentration of the citric acid in the R1 reagent is 0.15M, the concentration of the DMAB in the R1 reagent is 0.2mM; the concentration of the MBTH in the R1 reagent is 0.15mM;

[0070] R2 reagents include the following components:

[0071] The buffer solution, oxalate oxidase and peroxidase that pH value is 5; The concentration of described buffer solution in R2 reagent is 0.13M, and the concentration of described oxalate oxidase in R2 reagent is 200U / L, and described process The concentration of oxidase in the R2 reagent is 8000U / L; the buffer is a citrate buffer, and the citrate buffer has 0.13M citric acid and 0.13M sodium citrate in ...

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