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Method for enriching anaerobic ammonium oxidation bacteria by adding exogenous signal molecules

An exogenous signal molecule, anaerobic ammonium oxidation technology, applied in anaerobic digestion treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve unresolved and increase the abundance of anammox bacteria, etc. Achieve the effect of increasing abundance, promoting denitrification performance and improving sludge activity

Active Publication Date: 2021-11-12
HANGZHOU NORMAL UNIVERSITY
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Problems solved by technology

[0007] None of the above patent technical documents is aimed at the anammox reactor that is already in the stable operation stage, nor does it solve the problem of how to further increase the abundance of anammox bacteria in the anammox reactor that is already in the stable operation stage

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  • Method for enriching anaerobic ammonium oxidation bacteria by adding exogenous signal molecules
  • Method for enriching anaerobic ammonium oxidation bacteria by adding exogenous signal molecules

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Embodiment Construction

[0026] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The operating methods not indicated in the following examples are generally in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.

[0027] A method for enriching anammox bacteria by adding exogenous signal molecules, comprising the steps of:

[0028] (1) Use an upflow anaerobic sludge bed (UASB) reactor with an effective volume of 1 L, inoculate anaerobic ammonium oxidation flocculent sludge with a particle size of less than 0.9 mm, and the initial sludge volume is 0.5 L. Place it in a constant temperature incubator at 35±1°C and run it away from light, and the hydraulic retention time is 2 to 3 hours. The concentration of volatil...

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Abstract

The invention discloses a method for enriching anaerobic ammonium oxidation bacteria by adding exogenous signal molecules, which comprises the following steps: inoculating anaerobic ammonium oxidation sludge of which the functional bacteria are Candidatus Kuenenia in a reactor, taking ammoniacal nitrogen and nitrite nitrogen with the concentration of 250-300mg / L as water inlet substrates, adding inorganic salts and trace elements at the same time, adding 20-40 [mu] g L<-1> exogenous signal molecules C6-HSL into water after a reactor operates stably, continuously operating, enriching the functional bacteria Candidatus Kuenenia, and continuously improving the abundance of the functional bacteria Candidatus Kuenenia in the anaerobic ammonia oxidation sludge for a long time.

Description

technical field [0001] The invention relates to the technical field of ammonium oxidizing bacteria enrichment, in particular to a method for enriching anammox bacteria by adding exogenous signal molecules. Background technique [0002] The anammox process is a chemoautotrophic denitrification process in which anammox bacteria use nitrate as an electron acceptor to convert ammonia into nitrogen under anaerobic or anoxic conditions. Compared with the traditional nitrification-denitrification process, the anammox process has the advantages of high nitrogen removal efficiency, low oxygen consumption, low sludge production, and even no need for organic carbon replenishment. [0003] Anammox bacteria are spherical bacteria with an average diameter of 800-1100nm and are generally red. At present, laboratories mostly use reactors such as sequencing batch reactors, upflow anaerobic sludge bed (UASB) reactors, and biological turntables to cultivate anammox bacteria. However, due to ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F3/34
CPCC02F3/28C02F3/34Y02W10/10
Inventor 范念斯付瑾瑾卢郑扬马渊珑徐贤雯金仁村
Owner HANGZHOU NORMAL UNIVERSITY
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