Anti-inflammatory composition
A composition and compound technology, applied in anti-inflammatory agents, drug combinations, plant raw materials, etc., can solve problems such as increasing the incidence of cancer, infection, and increasing cytokine activity
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Embodiment 1
[0105] Fermented plant materials, including fermented rapeseed species (rapeseed) and seaweed (Laminaria) combined methanol extracts (80% methanol)) for compound analysis.
[0106] Chromatography at Dionex Performed on a 3000 Quaternary Rapid Separation UHPLC+focusing system (Thermo Fisher Scientific, Gemmering, Germany).
[0107] On a Kinetex 1.7u XB-C18 column (100×2.1mm, 1.7μm, Separation was achieved on Phenomenex, Torrance, CA, USA. Formic acid (0.05%) and acetonitrile (provided with 0.05% formic acid) in water were used as mobile phases A and B, respectively.
[0108] Gradient conditions are as follows: 0.0-0.5min, 2% B; 0.5-14.0min 2-20% B; -26.55min 100-2%B and 26.55-30.0min 2%B. The mobile phase flow rate was 300 μl min-1. The column temperature was maintained at 25°C. Four wavelengths (205nm, 220nm, 250nm and 390nm) were monitored by a UV-VIS detector.
[0109] Liquid chromatography was coupled to a CompactmicrOTOF-Q mass spectrometer (Bruker, Bremen, Germa...
Embodiment 2
[0122] Preparation of composite plant material extracts
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