Hook effect-free immunochromatography test strip and preparation method thereof
A technology of immunochromatography test paper and hook effect, which is applied in the direction of analyzing materials, measuring devices, instruments, etc., can solve the problems of incompletely solving the problem of hook effect and limiting the detection range, etc., to solve the hook effect and realize the switch effect , high detection specificity
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[0028] An immunochromatographic test strip without hook effect, comprising a bottom plate 1, a nitrocellulose membrane 2, a sample pad 3 and an absorption pad 4 are arranged above the bottom plate 1, and the nitrocellulose membrane 2 is located between the sample pad 3 and the absorption pad 4 Between, above the nitrocellulose membrane 2, a transition pad 5, a test line, and a control line are set, the test line is set between the transition pad 5 and the control line, a conjugate pad 6 is set above the transition pad 5, and a Set with labeled antibody.
[0029] Wherein, the transition pad 5 is a film processed by a chemical method or a film processed by a physical method, and the porosity is distributed in gradients.
[0030] The present invention also provides a method for preparing an immunochromatographic test strip without a hook effect, comprising the following steps:
[0031] Step 1: Fix the test line and the control line on the nitrocellulose membrane 2, and dry at 36...
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