Hook effect-free immunochromatography test strip and preparation method thereof

A technology of immunochromatography test paper and hook effect, which is applied in the direction of analyzing materials, measuring devices, instruments, etc., can solve the problems of incompletely solving the problem of hook effect and limiting the detection range, etc., to solve the hook effect and realize the switch effect , high detection specificity

Pending Publication Date: 2021-12-14
CHONGQING TECH & BUSINESS UNIV +2
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Although the dynamic measurement method is free from false negatives, it still limits the detection range of quantification
Therefore, none of these methods can completely solve the problems caused by the hook effect

Method used

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  • Hook effect-free immunochromatography test strip and preparation method thereof
  • Hook effect-free immunochromatography test strip and preparation method thereof
  • Hook effect-free immunochromatography test strip and preparation method thereof

Examples

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Effect test

Embodiment

[0028] An immunochromatographic test strip without hook effect, comprising a bottom plate 1, a nitrocellulose membrane 2, a sample pad 3 and an absorption pad 4 are arranged above the bottom plate 1, and the nitrocellulose membrane 2 is located between the sample pad 3 and the absorption pad 4 Between, above the nitrocellulose membrane 2, a transition pad 5, a test line, and a control line are set, the test line is set between the transition pad 5 and the control line, a conjugate pad 6 is set above the transition pad 5, and a Set with labeled antibody.

[0029] Wherein, the transition pad 5 is a film processed by a chemical method or a film processed by a physical method, and the porosity is distributed in gradients.

[0030] The present invention also provides a method for preparing an immunochromatographic test strip without a hook effect, comprising the following steps:

[0031] Step 1: Fix the test line and the control line on the nitrocellulose membrane 2, and dry at 36...

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Abstract

The invention discloses a hook effect-free immunochromatography test strip and a preparation method thereof. The immunochromatography test strip comprises a bottom plate, a nitrocellulose membrane, a sample pad and an absorption pad are arranged above the bottom plate, and the nitrocellulose membrane is located between the sample pad and the absorption pad. A transition pad, a test line and a control line are arranged above the nitrocellulose membrane, the test line is arranged between the transition pad and the control line, a conjugate pad is arranged above the transition pad, and a labeled antibody is arranged above the conjugate pad. Under the condition of no need of additional manual operation, the hook effect in a traditional sandwich method LFIA test strip is solved at low cost, the false negative in the test result is effectively eliminated, and the application limitation of the existing LFIA test strip is broken; the novel immune lateral flow chromatography test strip shows extremely high detection specificity on an analyte.

Description

technical field [0001] The invention belongs to the technical field of in vitro detection, in particular to an immunochromatographic test strip without hook effect and a preparation method. Background technique [0002] Sandwich-based lateral flow immunoassay (LFIA) technology has been widely developed in the field of in vitro detection due to its low cost, fast effect, and easy operation, and can be used for qualitative or quantitative analysis. The LFIA technology for qualitative analysis is mostly based on colorimetry, using the surface plasmon resonance phenomenon of gold nanoparticles for detection, and is suitable for application scenarios such as pregnancy, drug abuse, and infectious disease detection. The LFIA technology part of quantitative analysis is similar to the colorimetric method of qualitative LFIA, which uses color depth to achieve quantitative detection; in recent years, a fluorescent method using quantum dots or fluorescent microspheres as labels has also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 董涛何国桢杨朝初图莉尔·伦宁达阿若·布兰斯塔德
Owner CHONGQING TECH & BUSINESS UNIV
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