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Method for detecting pesticide and metabolite residues thereof in traditional Chinese medicinal materials

A technology for Chinese medicinal materials and metabolites, applied in the field of pesticide detection, can solve the problems of complexity, poor repeatability, time-consuming and labor-intensive, and achieve high accuracy and repeatability.

Pending Publication Date: 2022-01-18
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex matrix of Chinese herbal medicines, most of the existing pretreatment methods are time-consuming and laborious, and have low accuracy and poor repeatability.

Method used

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  • Method for detecting pesticide and metabolite residues thereof in traditional Chinese medicinal materials
  • Method for detecting pesticide and metabolite residues thereof in traditional Chinese medicinal materials
  • Method for detecting pesticide and metabolite residues thereof in traditional Chinese medicinal materials

Examples

Experimental program
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Effect test

Embodiment 1

[0079] The Chinese medicinal material Ophiopogon japonicus to be tested was pulverized with a high-speed pulverizer and passed through a 40-mesh sieve for later use. Accurately weigh 2g of Chinese herbal medicine powder, add 10mL of water, vortex for 1min to obtain the first extraction product, add 10mL of water and 10mL of 1% by volume acetic acid acetonitrile, and vortex for 1min to obtain the second extraction product, add 6g of anhydrous Magnesium sulfate and 1.5g sodium chloride, shake immediately, and vortex for 1min. Centrifuge at 4000 rpm for 5 minutes, and use the supernatant as matrix extract. Take 2mL of matrix extract, add 150mg of anhydrous magnesium sulfate, 30mg of ethylenediamine-N-propylsilyl silanized silica gel and 30mg of octadecylsilane bonded silica gel, vortex and mix for 1 minute, then centrifuge at 10000rpm for 5 minutes, and take 1mL of the supernatant was passed through a 0.22μm organic filter membrane and added to the sample injection vial as the t...

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Abstract

The invention relates to a method for detecting pesticide residues in traditional Chinese medicinal materials, and the method comprises the following steps: S1, mixing a traditional Chinese medicinal material matrix, water and an acetic acid acetonitrile aqueous solution, and carrying out first extraction to obtain a first extract; S2, mixing the first extract with anhydrous magnesium sulfate and sodium chloride, and performing second extraction to obtain a second extract; S3, performing first centrifugation on the second extract, taking supernate as a matrix extracting solution, mixing the matrix extracting solution with a purifying agent, performing purification treatment, performing second centrifugation on the purified material, and taking supernate as a to-be-detected solution, wherein the purifying agent is prepared from anhydrous magnesium sulfate, ethylenediamine-N-propyl silanized silica gel and octadecyl silane bonded silica gel; and S4, determining the to-be-detected solution by using gas chromatography-tandem mass spectrometry and / or ultra-high performance liquid chromatography-tandem mass spectrometry. The method can realize simultaneous detection of 75 pesticides in traditional Chinese medicinal materials, and has high accuracy and repeatability.

Description

technical field [0001] This application relates to the field of pesticide detection, in particular, to a method for detecting residues of pesticides and their metabolites in Chinese herbal medicines. Background technique [0002] Chinese medicinal materials are the source of the Chinese medicine industry, the raw materials for the production of Chinese patent medicine industry, and the basis of traditional Chinese medicine for treating diseases. With the rapid development of the traditional Chinese medicine industry, the demand for traditional Chinese medicine resources is increasing day by day. Artificial planting of Chinese medicinal materials is an effective way to realize the regeneration and sustainable utilization of traditional Chinese medicine resources. At present, the overall development level of traditional Chinese medicine agriculture is relatively backward. The prevention and control of diseases and insect pests in the production of Chinese medicinal materials m...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/065
Inventor 范蓓李敏敏赵玉乐王凤忠崔莹金诺黄亚涛卢嘉马晓帆冷轶凡
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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