Anti-tumor experimental animal model and construction method thereof

Abstract

The invention discloses an anti-tumor experimental animal model and a construction method thereof. The construction method comprises the steps that a cancer specimen of a patient is immediately placed in an RPMI-1640 culture solution after being in vitro, tumor tissue is trimmed into tumor blocks, meanwhile, a prepared normal nude mouse is subjected to general anesthesia, diphenytoin sodium is diluted with normal saline, then intraperitoneal injection of the diphenytoin sodium is conducted, an incision is obliquely formed in the right rib, the abdominal cavity is opened, transplanted visceral organ cavities can be exposed, prepared human cancer tissue blocks are implanted, blood is sticked with cotton swabs, bleeding stopping is realized, sterilizing is carried out with alcohol cotton balls after obvious bleeding is avoided, incisions are continuously sutured, surrounding bloodstains can be wiped, the material is sterilized, and put back into a cage, and viscera is taken for histological examination when a preset observation period is reached. According to the anti-tumor experimental animal model and the construction method thereof, a cancer patient sample is directly taken out from a patient to construct the animal model, the occurrence condition of human tumors can be restored to the maximum extent, tumor heterogeneity is achieved, and the construction method is low in cost, short in period and good in repeatability.

Description

technical field [0001] The invention relates to the field of anti-tumor experimental animal models, in particular to an anti-tumor experimental animal model and a construction method thereof. Background technique [0002] Anti-tumor experimental model refers to the experimental model used to detect and evaluate the anti-tumor activity of drugs, which can be used to screen anti-tumor drugs. It is human cancer cells, observe the killing effect or proliferation inhibition effect of drugs on tumor cells; in vivo experiment, use experimental animal transplanted tumors to model human cancer tumors and transplant them into immunodeficiency animal models, and observe drug efficacy, including the inhibitory effect on tumor growth and life-prolonging effects of tumor-bearing animals; biological models for simulating tumors; molecular biology models, according to the characteristics of tumor cells, select new molecular targets and establish corresponding models and methods, without con...

AI Technical Summary

Problems solved by technology

[0002] Anti-tumor experimental model refers to the experimental model used to detect and evaluate the anti-tumor activity of drugs, which can be used to screen anti-tumor drugs. It is human cancer cells, observe the killing effect or proliferation inhibition effect of drugs on tumor cells; in vivo experiment, use experimental animal transplanted tumors to model human cancer tumors and transplant them into immunodeficiency animal models, and observe drug efficacy, including the inhibitory effect on tumor growth and life-prolonging effects of tumor-bearing animals; biological models for simulating tumors; molecular biology models, according to the characteristics of tumor cells, select new molecular targets and establish corresponding models and methods, without conscious artificial experiments in experimental animal populations A type of tumor that occurs naturally due to treatment is called spontaneous tumor. The type and incidence of spontaneous tumor may vary with the species, strain and type of experimental animals. In tumor experimental research, generally, it should be selected Experimental animal tumor models with high morbidity are used as research objects, otherwise they cannot be studied. Of course, tumor models with low morbidity are also useful and can be used as controls. The existing anti-tumor experimental animal models are too cumbersome to construct, expensive and The cycle is long, and most of them use injection to inject tumors, which is not conducive to subculture, transplantation and preservation, resulting in the need for repeated injections on different mice