The invention provides an organ model body, a preparation method and application thereof in preparation of a resectoscope resection training model, and relates to the technical field of medical teaching molds. The organ model body comprises a simulated organ cavity and a simulated organ pipeline, wherein one end of the simulated organ pipeline is connected with a closed passage of the simulated organ cavity, and meanwhile, the simulated organ cavity is mainly prepared from the heart of a livestock animal, so that the operation touch feeling and the force feedback of a doctor in minimally invasive surgery training are ensured, and the effect that a training scene is more real is achieved; moreover, the livestock animal heart serving as an in vitro animal organ has the advantages of being convenient to obtain, economical and practical. Therefore, the organ model body not only saves investment of places, cost, labor and the like, but also can routinize minimally invasive surgery training,ensures that doctors can perform repeated training for many times, and achieves sufficient skill proficiency.