Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cornu cervi pantotrichum polypeptide and preparation method thereof

A deer antler polypeptide and filtrate technology, applied in the field of polypeptide preparation, can solve the problems of poor anti-fatigue and anti-oxidation effects, and the anti-inflammatory effect needs to be further improved, so as to optimize the enzymatic hydrolysis sequence, improve the activities of anti-oxidation and enhance immunity, and improve the anti-inflammatory effect. High oxidative and immune-enhancing effects

Active Publication Date: 2022-02-11
JILIN BUSINESS & TECH COLLEGE
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-fatigue and anti-oxidation effects are poor, and the anti-inflammatory effect needs to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cornu cervi pantotrichum polypeptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A preparation method of ginseng polypeptide, comprising the steps of:

[0054] (1) Cut the velvet antler into small pieces, wash it with cold water until it has no blood color, grind it, add it to the acetic acid-sodium acetate buffer solution with pH=3.5, homogenize it for 10 minutes, and filter to obtain the filter residue A and filtrate A;

[0055] (2) Carry out enzymolysis of the filter residue A in 8 times the amount of water, according to the mass of water, first add 1% pepsin, adjust the pH to 1.5, enzymolyze at 30°C for 40min; then add 0.5% pineapple enzyme , adjust the pH to 6, enzymatic hydrolysis at 40°C for 40min; finally add 1% compound enzyme (the mass ratio of neutral protease, papain and trypsin is 1:2:1), adjust the pH to 5.5, and hydrolyze at 40°C Under enzymatic hydrolysis for 40min, filter to obtain filtrate B;

[0056] (3) Combine filtrate A and filtrate B, add ethanol until the alcohol content is 70%, let it stand for 2 hours, and centrifuge at 25...

Embodiment 2

[0059] A preparation method of ginseng polypeptide, comprising the steps of:

[0060] (1) Cut the velvet antler into small pieces, wash it with cold water until it has no blood color, grind it, add it to the acetic acid-sodium acetate buffer solution with pH=3.5, homogenize it for 20 minutes, and filter to obtain the filter residue A and filtrate A;

[0061] (2) Carry out enzymolysis of the filter residue A in 10 times the amount of water, according to the mass of water, first add 3% pepsin, adjust the pH to 2, and enzymolyze at 40°C for 20min; then add 2% pineapple enzyme , adjust the pH to be 8, enzymolysis at 60°C for 20min; finally add 3% compound enzyme (the mass ratio of neutral protease, papain and trypsin is 1:4:2), adjust the pH to be 8, at 60°C Under enzymatic hydrolysis for 20min, filter to obtain filtrate B;

[0062] (3) Combine filtrate A and filtrate B, add ethanol until the alcohol content is 80%, let it stand for 5 hours, and centrifuge at 3500r / min to obtain ...

Embodiment 3

[0065] A preparation method of ginseng polypeptide, comprising the steps of:

[0066] (1) Cut the velvet antler into small pieces, wash it with cold water until it has no blood color, grind it, add it to the acetic acid-sodium acetate buffer solution with pH=3.5, homogenize it for 15 minutes, and filter to obtain the filter residue A and filtrate A;

[0067] (2) Carry out enzymolysis of the filter residue A in 9 times the amount of water, according to the mass of water, first add 2% pepsin, adjust the pH to 2, and enzymolyze at 35°C for 30min; then add 1% pineapple enzyme , adjust the pH to 7, enzymolysis at 50°C for 30min; finally add 2% compound enzyme (the mass ratio of neutral protease, papain and trypsin is 1:3:2), adjust the pH to 6.5, Under enzymatic hydrolysis for 30min, filter to obtain filtrate B;

[0068] (3) Combine filtrate A and filtrate B, add ethanol until the alcohol content is 75%, let it stand for 3 hours, and centrifuge at 3000r / min to obtain supernatant C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of polypeptide preparation, and concretely relates to a cornu cervi pantotrichum polypeptide and a preparation method thereof. The preparation method of the cornu cervi pantotrichum polypeptide comprises the following steps: 1, crushing cornu cervi pantotrichum, then adding into homogenate, homogenizing, and filtering to obtain filter residue A and a filtrate A ; (2) performing enzymolysis on the filter residue A in water, and filtering to obtain a filtrate B; (3) combining the filtrate A and the filtrate B, and performing alcohol precipitation to obtain a supernatant C; and (4) carrying out ultrafiltration on the supernatant C to obtain a filtrate C, and drying to obtain the cornu cervi pantotrichum polypeptide. The cornu cervi pantotrichum polypeptide has the advantages of obvious anti-fatigue, anti-oxidation and immunity-enhancing effects and simple preparation process.

Description

technical field [0001] The invention relates to the technical field of polypeptide preparation, in particular to an antler polypeptide and a preparation method thereof. Background technique [0002] Antler is the unossified and densely hairy young horn of the stag Cervus nippon Temminck or red deer Cervus elaphusLinnaeus of the deer family. The former is commonly known as "flower antler", while the latter is commonly referred to as "red antler". "Shen Nong's Materia Medica" records that "Velvet antler is sweet and warm, non-toxic, the main leakage of bad blood, cold and heat convulsions, nourishing Qi and strong will, and the population is not old." "Compendium of Materia Medica" records: "Deer antler, produces essence and nourishes marrow, nourishes blood and Yang, strengthens muscles and bones, and cures all deafness, deafness, dark eyes, dizziness, and dysentery." [0003] The chemical composition of deer antler is relatively complex, including more than 19 kinds of ami...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30A61K38/01A61P39/00A61P39/06A61P37/04A61K36/8968
CPCC12P21/06C07K1/36C07K1/34C07K1/30A61K38/012A61K36/258A61K36/815A61K36/8968A61K36/54A61P39/00A61P39/06A61P37/04A61K2300/00Y02A50/30
Inventor 鞠凤霞殷金龙张卓郑卫王磊黄旭
Owner JILIN BUSINESS & TECH COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com