Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application of rhodococcus ruber HJM-8

A technology of dimethylacetamide, HJM-8, applied in the field of microorganisms, can solve problems such as difficult biodegradation, environmental and human toxicity

Pending Publication Date: 2022-02-15
ZHEJIANG SHUREN COLLEGE ZHEJIANG SHUREN UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DMAC has certain toxicity, and its discharge into the environment will have tox

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application of rhodococcus ruber HJM-8
  • Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application of rhodococcus ruber HJM-8
  • Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application of rhodococcus ruber HJM-8

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] The preparation method of described bacterium-containing suspension comprises the following specific steps:

[0027] (1) Incline culture: Inoculate Rhodococcus Erythrococcus HJM-8, which efficiently degrades dimethylacetamide, onto the slant medium, and culture it at 30-38°C for 1-3 days to obtain slant bacteria; the slant medium is finally Concentration composition is: K 2 HPO 4 1500mg·L -1 , KH 2 PO 4 500mg·L -1 , NaCl 1000mg·L -1 , DMAC 500mg·L -1 , MgSO 4 ·7H 2 O 200mg·L -1 , solvent is water, pH value 7.0-8.0, agar 18-20g L -1 ;

[0028] (2) Seed culture: pick colonies from the slant and inoculate them into the seed medium, and cultivate them at 30-38°C for 18-24h to obtain the seed liquid; the final concentration of the seed medium consists of: NaCl 10g L -1 , yeast extract powder 5g·L-1 , peptone 10g·L -1 , the solvent is water, the pH value is 7.0-8.0;

[0029] (3) Fermentation: inoculate the seed solution to the fermentation medium with an inocu...

Embodiment 1

[0033] Example 1: Isolation, purification and identification of Rhodococcus ruber (Rhodococcus ruber) HJM-8

[0034] 1. Isolation and purification of Rhodococcus ruber HJM-8

[0035] Rhodococcus ruber (Rhodococcus ruber) HJM-8 was screened from the aerobic pool sludge of a pharmaceutical wastewater treatment plant in Taizhou, Zhejiang. The specific steps are as follows:

[0036] (1) Sampling: Sampling at multiple points from the sludge in the aerobic tank of a pharmaceutical wastewater treatment plant in Zhejiang, as a raw material for screening Rhodococcus ruber (Rhodococcus ruber) HJM-8, which can efficiently degrade dimethylacetamide;

[0037] (2) Strain isolation: Take an appropriate amount of activated sludge in the aerobic tank, let it stand for 2 hours, take 10 mL of supernatant and inoculate it into a 250 mL culture bottle containing 100 mL of enriched culture solution, and shake it on a constant temperature shaker at 30°C and 160 rpm 24h, repeated 3 times, the final ...

Embodiment 2

[0046] Example 2 The preparation process of the bacterial suspension containing Rhodococcus rubrum HJM-8 that efficiently degrades dimethylacetamide as the active ingredient

[0047] The preparation process of the bacterium-containing suspension:

[0048] (1) Slant culture: inoculate the Rhodococcus erythrococcus HJM-8, which efficiently degrades dimethylacetamide, onto the slant medium, and culture it at 30° C. for 3 days to obtain slant cells; the final concentration of the slant medium consists of: K 2 HPO 4 1500mg·L -1 , KH 2 PO 4 500mg·L -1 , NaCl 1000mg·L -1 , DMAC 500mg·L -1 , MgSO 4 ·7H 2 O 200mg·L -1 , solvent is water, pH value 7.0, agar 18g L -1 ;

[0049] (2) Seed culture: pick colonies from the slant and inoculate them into the seed medium, and cultivate them at 30°C for 18 hours to obtain the seed liquid; the final concentration of the seed medium consists of: NaCl 10g L -1 , yeast extract powder 5g·L -1 , peptone 10g·L -1 , the solvent is water, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide (DMAC) and degradation application of the Rhodococcus ruber HJM-8. The Rhodococcus ruber HJM-8 is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the postal code is 430072, the preservation number is CCTCC NO: M 2021654, and the preservation date is June 1, 2021. According to the rhodococcus ruber HJM-8 with the DMAC degradation performance, the degradation rate of DMAC with the initial concentration being 500 mg.L <-1> can reach 100% within 24 h, and the discovery of the degradation bacterium has important significance on efficient purification of industrial wastewater containing DMAC.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Rhodococcus erythrococcus HJM-8 that efficiently degrades dimethylacetamide and its application. Background technique [0002] Dimethylacetamide (DMAC) is an important chemical raw material and a solvent with excellent performance. It is widely used in polyurethane, acrylic fiber, medicine, pesticide, dyestuff and electronics industries. DMAC has a certain degree of toxicity, and its discharge into the environment will have toxic effects on the environment and humans, and it is also difficult to biodegrade. [0003] DMAC is an important solvent in synthetic fibers such as acrylonitrile, polyurethane textiles and polyurethane resin synthesis industries. It can also be used as a solvent for C8 fractionation and separation of styrene extraction. Due to its strong miscibility, DMAC is used in medicine and polymer membranes. , The field of dye coating synthesis is also widel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC12N1/20C02F3/34C02F2101/38
Inventor 陈浚王泽宇袁博涵何佳美
Owner ZHEJIANG SHUREN COLLEGE ZHEJIANG SHUREN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap