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Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application of rhodococcus ruber HJM-8

A technology of dimethylacetamide, HJM-8, applied in the field of microorganisms, can solve problems such as difficult biodegradation, environmental and human toxicity

Pending Publication Date: 2022-02-15
ZHEJIANG SHUREN COLLEGE ZHEJIANG SHUREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DMAC has a certain degree of toxicity, and its discharge into the environment will have toxic effects on the environment and humans, and it is also difficult to biodegrade

Method used

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  • Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application of rhodococcus ruber HJM-8
  • Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application of rhodococcus ruber HJM-8
  • Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application of rhodococcus ruber HJM-8

Examples

Experimental program
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Effect test

preparation example Construction

[0026] The preparation method of described bacterium-containing suspension comprises the following specific steps:

[0027] (1) Incline culture: Inoculate Rhodococcus Erythrococcus HJM-8, which efficiently degrades dimethylacetamide, onto the slant medium, and culture it at 30-38°C for 1-3 days to obtain slant bacteria; the slant medium is finally Concentration composition is: K 2 HPO 4 1500mg·L -1 , KH 2 PO 4 500mg·L -1 , NaCl 1000mg·L -1 , DMAC 500mg·L -1 , MgSO 4 ·7H 2 O 200mg·L -1 , solvent is water, pH value 7.0-8.0, agar 18-20g L -1 ;

[0028] (2) Seed culture: pick colonies from the slant and inoculate them into the seed medium, and cultivate them at 30-38°C for 18-24h to obtain the seed liquid; the final concentration of the seed medium consists of: NaCl 10g L -1 , yeast extract powder 5g·L-1 , peptone 10g·L -1 , the solvent is water, the pH value is 7.0-8.0;

[0029] (3) Fermentation: inoculate the seed solution to the fermentation medium with an inocu...

Embodiment 1

[0033] Example 1: Isolation, purification and identification of Rhodococcus ruber (Rhodococcus ruber) HJM-8

[0034] 1. Isolation and purification of Rhodococcus ruber HJM-8

[0035] Rhodococcus ruber (Rhodococcus ruber) HJM-8 was screened from the aerobic pool sludge of a pharmaceutical wastewater treatment plant in Taizhou, Zhejiang. The specific steps are as follows:

[0036] (1) Sampling: Sampling at multiple points from the sludge in the aerobic tank of a pharmaceutical wastewater treatment plant in Zhejiang, as a raw material for screening Rhodococcus ruber (Rhodococcus ruber) HJM-8, which can efficiently degrade dimethylacetamide;

[0037] (2) Strain isolation: Take an appropriate amount of activated sludge in the aerobic tank, let it stand for 2 hours, take 10 mL of supernatant and inoculate it into a 250 mL culture bottle containing 100 mL of enriched culture solution, and shake it on a constant temperature shaker at 30°C and 160 rpm 24h, repeated 3 times, the final ...

Embodiment 2

[0046] Example 2 The preparation process of the bacterial suspension containing Rhodococcus rubrum HJM-8 that efficiently degrades dimethylacetamide as the active ingredient

[0047] The preparation process of the bacterium-containing suspension:

[0048] (1) Slant culture: inoculate the Rhodococcus erythrococcus HJM-8, which efficiently degrades dimethylacetamide, onto the slant medium, and culture it at 30° C. for 3 days to obtain slant cells; the final concentration of the slant medium consists of: K 2 HPO 4 1500mg·L -1 , KH 2 PO 4 500mg·L -1 , NaCl 1000mg·L -1 , DMAC 500mg·L -1 , MgSO 4 ·7H 2 O 200mg·L -1 , solvent is water, pH value 7.0, agar 18g L -1 ;

[0049] (2) Seed culture: pick colonies from the slant and inoculate them into the seed medium, and cultivate them at 30°C for 18 hours to obtain the seed liquid; the final concentration of the seed medium consists of: NaCl 10g L -1 , yeast extract powder 5g·L -1 , peptone 10g·L -1 , the solvent is water, ...

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Abstract

The invention discloses Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide (DMAC) and degradation application of the Rhodococcus ruber HJM-8. The Rhodococcus ruber HJM-8 is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the postal code is 430072, the preservation number is CCTCC NO: M 2021654, and the preservation date is June 1, 2021. According to the rhodococcus ruber HJM-8 with the DMAC degradation performance, the degradation rate of DMAC with the initial concentration being 500 mg.L <-1> can reach 100% within 24 h, and the discovery of the degradation bacterium has important significance on efficient purification of industrial wastewater containing DMAC.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Rhodococcus erythrococcus HJM-8 that efficiently degrades dimethylacetamide and its application. Background technique [0002] Dimethylacetamide (DMAC) is an important chemical raw material and a solvent with excellent performance. It is widely used in polyurethane, acrylic fiber, medicine, pesticide, dyestuff and electronics industries. DMAC has a certain degree of toxicity, and its discharge into the environment will have toxic effects on the environment and humans, and it is also difficult to biodegrade. [0003] DMAC is an important solvent in synthetic fibers such as acrylonitrile, polyurethane textiles and polyurethane resin synthesis industries. It can also be used as a solvent for C8 fractionation and separation of styrene extraction. Due to its strong miscibility, DMAC is used in medicine and polymer membranes. , The field of dye coating synthesis is also widel...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC12N1/20C02F3/34C02F2101/38
Inventor 陈浚王泽宇袁博涵何佳美
Owner ZHEJIANG SHUREN COLLEGE ZHEJIANG SHUREN UNIV
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