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Countercurrent chromatography continuous sampling separation method based on elution-extrusion mode

A technology of countercurrent chromatography and separation method, which is applied in the field of natural product separation, and can solve problems such as inability to separate target compounds at the same time, achieve the effects of reducing solvent consumption, rapid separation process, and increased preparation volume

Pending Publication Date: 2022-03-01
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, existing elution-extrusion modes cannot simultaneously separate two target compounds

Method used

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  • Countercurrent chromatography continuous sampling separation method based on elution-extrusion mode
  • Countercurrent chromatography continuous sampling separation method based on elution-extrusion mode
  • Countercurrent chromatography continuous sampling separation method based on elution-extrusion mode

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Embodiment 1

[0030] Continuous sample separation, using the elution extrusion mode, first pump the stationary phase (upper phase) into the countercurrent chromatography separation column at a flow rate of 30mL / min; after the countercurrent chromatography separation column is filled with the stationary phase, the countercurrent chromatography Set to 1600rpm, turn on the countercurrent chromatograph; when the actual speed of the countercurrent chromatograph reaches 1600rpm and is stable, pump the mobile phase into the countercurrent chromatographic separation column at a flow rate of 6.0mL / min; at the same time, calculate the continuous pump according to the formula (1) Into the sample solution time T inj is 14.87 minutes. In order to ensure that the elution and separation of the two compounds in the sample solution do not overlap, the continuous injection time is set to 14 minutes. After the stationary phase and mobile phase in the countercurrent chromatographic column reach hydrodynamic e...

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Abstract

The invention discloses a counter-current chromatography continuous sampling separation method based on an elution-extrusion mode. An elution-extrusion mode in counter-current chromatography is adopted, a sample is dissolved by a mobile phase in a two-phase solvent of the counter-current chromatography, and continuous sample injection of the sample is realized. The method is mainly used for separating two compounds, one compound can be obtained through separation in the elution process, and the other compound can be obtained through separation in the extrusion process. According to the preparation method disclosed by the invention, real continuous sample introduction is realized by utilizing an elution-extrusion mode in counter-current chromatography, and compared with a traditional method, the sample introduction amount is greatly increased, the solvent consumption can be reduced, and the preparation amount can be increased.

Description

technical field [0001] The invention belongs to the technical field of separation methods of natural products, and relates to a continuous sampling separation method of countercurrent chromatography, in particular to a continuous sampling separation method of countercurrent chromatography based on an elution-extrusion mode. Background technique [0002] Counter-current chromatography (Counter-current Chromatography) is a chromatographic separation technique based on the principle of liquid-liquid distribution. It mainly uses the difference in the distribution coefficient of the target compound between immiscible two-phase solvents to achieve separation. Due to the use of liquid stationary phase and mobile phase, it has the advantages of no irreversible adsorption caused by solid stationary phase, large sample volume, simple sample pretreatment, etc., and has been widely used in the field of natural product separation. [0003] Elution-extrusion mode (Elution-Extrusion Mode) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40B01D15/08B01D15/10B01D15/42
CPCC07D311/30C07D311/40B01D15/08B01D15/10B01D15/42
Inventor 黄新异邸多隆孙潇
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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