Unlock instant, AI-driven research and patent intelligence for your innovation.
Novel ligand assays
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
一种配体、受体的技术，应用在新型配体测定领域，能够解决昂贵、费用高昂等问题
Pending Publication Date: 2022-03-04
INSTUGEN GMBH
View PDF1 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, preparation of nuclear extracts from eukaryotic cells is expensive to manufacture due to the need for expensive cell growth media and the technical expertise required to enrich nuclear material
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
example 1
[0430] The results presented in Example 1, Table 1 directly compare the effect of manipulating the stoichiometry between steroid hormone receptors and nucleic acid reporter constructs for the same 15bp construct comprising a Cy3 / Cy5 fluorophore combination at the opposite end , when the ratio of steroid hormone receptor to reporter construct / HRE increased from 0.423 to 2.12 in the presence of SHR ligand, ΔE FRET Increased from 15% to 34%. This represents ΔE FRET Significantly and thus assay sensitivity is enhanced.
[0431] Thus, in further examples according to the test kits, assays and methods described herein, the ratio of steroid hormone receptor to nucleic acid reporter construct is between about 1:1 and about 5.0:1 and includes but is not limited to : 1.0:1, 1.1:1, 1.2:1, 1.3:1, 1.4:1, 1.5; 1, 1.6:1, 1.7:1, 1.8:1, 1.9:1, 2.0:1, 2.1:1, 2.2 :1, 2.3:1, 2.4:1, 2.5; 1, 2.6:1, 2.7:1, 2.8:1, 2.9:1, 3.0:1, 3.1:1, 3.2:1, 3.3:1, 3.4:1 , 3.5; 1, 3.6:1, 3.7:1, 3.8:1, 3.9:1, 4.0:...
example
[0645] The following information and data demonstrate the potential for ligands that bind to and activate the androgen receptor, including, for example, testosterone and dihydrotestosterone, or bind to the estrogen receptor, including, for example, estradiol. And various prototype assays for the detection of ligands that activate estrogen receptors. These examples serve to illustrate the activity assay platform described and claimed herein, in which ligands that bind to the androgen receptor or ligands that bind to the estrogen receptor (i.e., ER-α and ER-β) The assay concepts and principles of detection exemplified will be equally applicable to the detection of other receptor ligands of interest, including but not limited to ligands that bind to progesterone receptors including but not limited to progesterone, Ligands that bind to mineralocorticoid receptors including but not limited to aldosterone and ligands that bind to glucocorticoid receptors including but not limited to...
example 2
[0685] Other Fluorometric Prototypes
[0686] 2.1 Fluorescence adjustment via AR / ARE platform
[0687] The main components of fluorescence modulation by the AR / ARE platform include a DNA construct encoding an androgen response element (ARE) and incorporating a fluorescent moiety and a quencher moiety, combined with recombinant androgen receptor (AR), recombinant heat Shock protein 90 (HSP90) and buffer combination.
[0688] When the ARE is bound by the ligand-AR, the DNA construct will exhibit a high level of fluorescence modulation, resulting in a change in the level of fluorescence.
[0689] · DNA constructs include
[0690] o Incorporating ARE SEQ ID No.1 and 2 oligonucleotides
[0691] o A fluorophore dye and a fluorescent quencher matched to the fluorophore dye
[0692] ·Business restructuring AR
[0693] · Commercial recombinant HSP90
[0694] · Androgen ligands (such as testosterone)
[0695] • Necessary buffers.
[0696] 2.2 Fluorescence modulation by AR / ARE
...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The present invention relates to the detection of ligands that bind to and activate a steroid hormone receptor. In particular, the present invention provides test kits and assays for selectively identifying steroid hormone receptor ligands from a test sample. Importantly, the test kits and assays described herein are cell-free and enzyme-free and do not require the production of expensive nuclear extracts to enable the performance of the test kits and assays. In contrast, the test kits and assays described herein employ a reporter construct that includes a hormone response element that, when bound by a ligand-activated steroid hormone receptor, changes physical, mechanical, optical, photochemical, or electrochemical properties of the reporter construct. Thus, the presence of a target ligand in a sample under study can be determined using measured changes in physical, mechanical, optical, photochemical, or electrochemical properties (e.g., fluorescent readings) of the reporter construct.
Description
technical field [0001] The present invention generally relates to assays, methods and test kits for detecting ligands in a sample. In particular, the present invention provides assays, methods and test kits for screening test samples for the presence of a ligand characterized by its ability to form a complex with a steroid hormone receptor and elicit a steroid hormone-specific genomic response. Background technique [0002] The detection of ligands capable of eliciting a genomic response to steroid hormones is important in many fields of biochemistry, molecular biology and medicine. Such ligands include endogenous steroids, exogenous steroids, non-steroidal molecules and synthetic molecules. For example, determination of total hormone bioactivity in serum or plasma is important for monitoring human health-related conditions including aging, perimenopause, menopause, androgen deficiency, androgen hyperactivity, hormone replacement therapy , endocrine cancers including breas...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More