Preparation method of antibacterial gel with polyethyleneimine as gel matrix

A polyethylenimine and gel matrix technology, applied in bandages, medical science, etc., can solve the problems of high difficulty in process control, complicated hydrogel preparation process, poor antibacterial effect of hydrogel, etc., and achieve good inhibitory effect, The effect of good application prospects

Active Publication Date: 2022-03-22
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chitosan, hyaluronic acid, sodium alginate, collagen and other natural polymers are common raw materials for preparing hydrogel dressings. Most of these natural polymers have certain antibacterial activity and can endow hydrogel with antibacterial properties. The preparation process of the hydrogel is relatively complicated, the process control is difficult, and the antibacterial effect of the prepared hydrogel is poor. Therefore, a medical gel material with a relatively simple preparation method and good antibacterial effect is needed.

Method used

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  • Preparation method of antibacterial gel with polyethyleneimine as gel matrix
  • Preparation method of antibacterial gel with polyethyleneimine as gel matrix
  • Preparation method of antibacterial gel with polyethyleneimine as gel matrix

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A kind of preparation method that takes polyethyleneimine as the antibacterial gel of gel base, comprises the following steps:

[0035] Step 1. Add polyethyleneimine to 10mmol / L Tris-Hcl buffer to prepare a polyethyleneimine solution with a concentration of 64mg / mL; add catechol to 10mmol / L Tris-Hcl buffer In, the preparation concentration is the catechol solution of 26.4mg / mL;

[0036] Step 2, 5g polyethyleneimine solution, 5g catechol solution, 1g chitosan, 0.1g carbomer, 0.1g hyaluronic acid, 0.1g sodium benzoate, 0.05g konjac glucomannan and 0.1g H with a mass fraction of 30% 2 o 2 After mixing, stir at a speed of 1500r / min for 10min, and then pressurize and ultrasonicate for 60min to obtain an antibacterial gel; the pressure of the pressurized ultrasonic is 0.5MPa; the ultrasonic frequency is 75KHz, and the ultrasonic power is 300W;

[0037] After testing, the antimicrobial gel obtained in this example has a bacteriostatic rate of 97.1% and 97.4% for Escherichia...

Embodiment 2

[0039] A kind of preparation method that takes polyethyleneimine as the antibacterial gel of gel base, comprises the following steps:

[0040] Step 1. Add polyethyleneimine to 10mmol / L Tris-Hcl buffer to prepare a polyethyleneimine solution with a concentration of 64mg / mL; add catechol to 10mmol / L Tris-Hcl buffer In, the preparation concentration is the catechol solution of 26.4mg / mL;

[0041] Step 2, with 6g polyethyleneimine solution, 6g catechol solution, 1.5g chitosan, 0.2g carbomer, 0.2g hyaluronic acid, 0.2g sodium benzoate, 0.1g konjac glucomannan and 0.2 g with a mass fraction of 30% H 2 o 2 After mixing, stir at a speed of 2000r / min for 10min, and then pressurize and ultrasonicate for 45min to obtain an antibacterial gel; the pressure of the pressurized ultrasonic is 0.6MPa; the ultrasonic frequency is 75KHz, and the ultrasonic power is 300W;

[0042] After testing, the antibacterial rates of the antibacterial gel obtained in this example against Escherichia coli a...

Embodiment 3

[0044] A kind of preparation method that takes polyethyleneimine as the antibacterial gel of gel base, comprises the following steps:

[0045] Step 1. Add polyethyleneimine to 10mmol / L Tris-Hcl buffer to prepare a polyethyleneimine solution with a concentration of 64mg / mL; add catechol to 10mmol / L Tris-Hcl buffer In, the preparation concentration is the catechol solution of 26.4mg / mL;

[0046]Step 2, 8g polyethyleneimine solution, 7g catechol solution, 2g chitosan, 0.3g carbomer, 0.2g hyaluronic acid, 0.2g sodium benzoate, 0.1g konjac glucomannan and 0.1g H with a mass fraction of 30% 2 o 2 After mixing, stir at a speed of 2000r / min for 10min, and then pressurize and ultrasonicate for 45min to obtain an antibacterial gel; the pressure of the pressurized ultrasonic is 0.6MPa; the ultrasonic frequency is 75KHz, and the ultrasonic power is 300W;

[0047] After testing, the antibacterial rates of the antibacterial gel obtained in this example to Escherichia coli and Staphylococ...

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Abstract

The invention discloses a preparation method of antibacterial gel taking polyethyleneimine as a gel matrix. The preparation method comprises the following steps: preparing a polyethyleneimine solution and a catechol solution; the preparation method comprises the following steps: mixing a polyethyleneimine solution, a catechol solution, chitosan, carbomer, hyaluronic acid, sodium benzoate, konjac glucomannan and H2O2, stirring, and carrying out pressurized ultrasonic treatment to obtain the antibacterial gel. The antibacterial gel taking the polyethyleneimine as the gel matrix has a very good inhibition effect on staphylococcus aureus and escherichia coli, and in addition, the antibacterial gel material disclosed by the invention does not inhibit cell activity and has an excellent application prospect.

Description

technical field [0001] The invention relates to the technical field of medical dressings, in particular to a method for preparing an antibacterial gel using polyethyleneimine as a gel matrix. Background technique [0002] The skin is an important barrier for the human body to resist external damage. Skin defects caused by mechanical trauma, deep burns, chronic ulcers, etc. have always been a difficult problem for clinicians. Traditional dressings such as gauze, bandages, etc. can reduce pollution and protect the wound through compression to stop bleeding; however, the function is single, and it is easy to cause wound damage during the dressing change process. Using gel as a dressing is a better protection method. The gel material used in the medical field needs to have good moisture retention; in addition, it is necessary to prevent the growth and reproduction of bacteria; other gel materials can provide anti-inflammatory, antibacterial, and hemostatic , Promote the growth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/008A61L26/0014A61L26/0023A61L26/0004A61L26/0066A61L2300/404C08L79/02C08L5/08
Inventor 穆宁黄晓蓓闫肃李礼杨大坚王化斌
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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