Method for long-term conservation and rapid recovery of reniform worm strains

Abstract

The invention belongs to the field of long-term preservation of germplasm resources of functional species, and particularly relates to a long-term breed conservation and rapid recovery method for reniform worm strains, which is characterized in that a 0.22 mu m microporous filter membrane is selected as a breed conservation medium. According to the breed conservation method, the reniform worm bodies can enter the cysts and are attached to the filter membranes in a short time, reniform worm seed plants can be subjected to breed conservation stably for a long time, and compared with a traditional breed conservation method, the method is more convenient and faster, and has the advantages of being longer in preservation time, relatively easy to achieve breed conservation conditions, lower in cost, convenient to transport and carry and the like. The invention also relates to a method for obtaining high-density reniform worm by resuscitation of the microporous filter membrane, the resuscitation process is simple, the resuscitation process is not limited by temperature and time, and the effect is remarkable. The stable breed conservation validity period of the reniform worm strain can reach more than half a year, and the problems of tedious breed conservation procedures, space limitation, death of worm bodies in the transportation process and the like are effectively solved.

Description

technical field [0001] The invention belongs to the field of long-term preservation of germplasm resources of functional species, and in particular relates to a method for long-term preservation and rapid recovery of reniform species strains, using a 0.22 μm microporous filter membrane for long-term preservation and preservation of reniform species strains Afterwards, the method of resuscitating the sample with distilled water and sterilized wheat kernels. Background technique [0002] Species, species preservation method, conservation purpose and effect in the species preservation method of free-living ciliates reported in the prior art are as follows: [0003] [0004] The main defects of existing seed conservation methods include: [0005] (1) A long-term conservation and high-efficiency recovery method for Nephromorpha has not been established. [0006] (2) In the past, the general conservation method of free-living ciliates was used, which required high equipment a...

AI Technical Summary

Problems solved by technology

[0005] (1) A long-term conservation and high-efficiency recovery method for nephromorpha has not been established

[0006] (2) In the past, the general conservation method of free-living ciliates was used, which required high equipment and conditions, poor stability, short conservation time, and required regular maintenance and repeated recovery to maintain activity;

[0007] (3) The previous methods of seed preservation have high requirements on transportation conditions and cannot tolerate long-distance and high-temperature and low-temperature environments

[0008] The preservation of protozoan species is very important, and it is an important prerequisite for all scientific research work using protozoa as experimental materials. At present, the general conservation methods for ciliates are not ideal for the preservation of reniform species. How to It is the problem to be solved in the present invention that convenient and efficient long-term preservation and rapid recovery of the nephroid strains are carried out

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