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Method for cryopreservation and resuscitation of follicles

A technology of cryopreservation and cryoprotectant, which is applied in the field of cryopreservation and resuscitation of follicles, and can solve problems such as devitrification, recrystallization, and insufficient rewarming rate

Active Publication Date: 2022-07-08
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method does not rewarm quickly enough, leading to severe devitrification and recrystallization, which can be fatal for preantral follicles

Method used

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  • Method for cryopreservation and resuscitation of follicles
  • Method for cryopreservation and resuscitation of follicles
  • Method for cryopreservation and resuscitation of follicles

Examples

Experimental program
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Embodiment 1

[0038] The methods of microencapsulation, cryopreservation, nano-rewarming and in vitro 3D culture of preantral follicles are as follows:

[0039] 1) Hydrogel microencapsulation of preantral follicles: performed by a small centrifuge device. The centrifuge device consists of a 1.5mL special centrifuge tube and nozzle. The nozzle (300 μm in diameter) was fixed on the special centrifuge tube through the hole in the tube cap. First, a 0.3 mol / L calcium chloride solution was added to a special centrifuge tube, and a sodium alginate solution (concentration of 1 wt%) containing preantral follicles was put into the nozzle, and centrifuged at 100 × g for 2 minutes to obtain the resulting Alginate hydrogel microcapsules loaded with preantral follicles, which were then collected into culture medium for subsequent manipulation.

[0040] 2) Incubation of protective agent and addition of nanoparticles: Immerse the encapsulated preantral follicles in a cryoprotectant composed of water and...

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Abstract

The invention relates to the technical field of follicle cryopreservation, in particular to a follicle cryopreservation and resuscitation method. According to the method for cryopreserving the follicles, the follicles are firstly packaged, and hydrogel packaging can effectively prevent ice crystals from growing inwards, so that extracellular ice damage is reduced, and the concentration of a permeable protective agent is reduced. Due to isolation of the hydrogel, the ovarian follicles cannot be toxic by adding the nanoparticles into the cryopreservation liquid, and the ovarian follicles can be heated uniformly by performing magnetic induction heating and light induction heating on the cryopreserved ovarian follicles through the nanoparticles, so that the resuscitation efficiency is improved, and the survival rate of the resuscitated ovarian follicles is increased. In addition, the three-dimensional network structure of the encapsulated hydrogel is similar to an extracellular matrix, and the hydrogel can be used for in-vitro culture of anterior follicles after resuscitation.

Description

technical field [0001] The present invention relates to the technical field of cryopreservation of follicles, in particular to a cryopreservation and recovery method of follicles. Background technique [0002] In recent years, some malignant diseases (such as breast cancer, blood diseases) have an increasing incidence in young women. While aggressive chemotherapy, radiation therapy or bone marrow transplants can cure 90 percent of girls and young women, these treatments can still damage gonadal function and even lead to premature ovarian failure. Consequently, the demand for female fertility preservation has increased dramatically. Cryopreservation of preantral follicles is a safe option for prepubertal girls with specific diseases (eg, acute leukemia, ovarian cancer) and women in urgent need of cancer treatment. [0003] The development of follicles is generally divided into primordial follicles, primary follicles, secondary follicles, tertiary follicles and antral follic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/071
CPCA01N1/0221A01N1/0231A01N1/0294A01N1/0284C12N5/0682C12N2529/00C12N2501/31C12N2500/25C12N2501/235C12N2501/11C12N2531/00A01N1/02
Inventor 赵刚田聪会沈凌霄
Owner UNIV OF SCI & TECH OF CHINA
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