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High-specificity permeability-scattering integrated method ferritin latex turbidimetric detection kit

A detection kit and high-specificity technology, applied in the field of high-specificity transmission-scattering integrated method ferritin latex turbidimetric detection kit, can solve the problems of non-specific signal value occurrence, inconsistent transmission results, etc., achieve fast speed and low cost Low, high-throughput effects

Pending Publication Date: 2022-03-22
BYRON DIAGNOSTICS SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The R&D personnel of Bo Rong Diagnostics and Hitachi High-tech Co., Ltd. jointly found that the traditional transmission platform reagents have no problems in the selection of sensitizer type and concentration, and the selection of surfactants. If they are directly applied to the scattering platform, it will lead to non-specific signals. values ​​appear, causing inconsistencies between scattering and transmission results

Method used

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  • High-specificity permeability-scattering integrated method ferritin latex turbidimetric detection kit
  • High-specificity permeability-scattering integrated method ferritin latex turbidimetric detection kit
  • High-specificity permeability-scattering integrated method ferritin latex turbidimetric detection kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0146] The first reagent R1 contains components at the following concentrations:

[0147] 100mM Hepes-NaOH buffer, pH7.0

[0148] NaCl 1800mM

[0149] Polyethylene glycol 120002g / L

[0150] Tween-2016ml / L

[0151] Deferoxamine 10g / L

[0152] Rabbit polyclonal antibody blocking agent 40ml / L (Fei Peng Bio E-015)

[0153] Preservative 0.5ml / L PC300

[0154] The corresponding R2 contains the following concentrations of components:

[0155] 50mM PBS pH7.4

[0156] Tween-2016ml / L

[0157] 200nm carboxyl latex 2.4g / L

[0158] 350nm carboxyl latex 1.8g / L

[0159] Protective agent (sucrose 30g / L, trehalose 10g / L, fructose 10g / L)

[0160] FER rabbit polyclonal antibody 40mg / L (Chengdu Huirui Xinyuan Biotechnology)

[0161] Preservative 0.5ml / L PC300

[0162] Among them, the carboxyl latex latex is subjected to secondary convergent polymerization according to 2017109869707 "A Method for Improving the Antigen Excess and Linear Range of Latex Immunoturbidimetry"; Sensitivity p...

Embodiment 2

[0187] The first reagent R1 contains components at the following concentrations:

[0188] 100mM Hepes-NaOH buffer, pH6.8

[0189] NaCl 1500mM

[0190] Polyethylene glycol 120002g / L

[0191] Tween-2016ml / L

[0192] Deferoxamine 15g / L

[0193] Rabbit polyclonal antibody blocking agent 20ml / L (Fei Peng Bio E-015)

[0194] Preservative 0.5ml / L PC300

[0195] The corresponding R2 contains the following concentrations of components:

[0196] 100mM BES-NaOH buffer pH7.0

[0197] Tween-2010ml / L

[0198] 200nm carboxyl latex 1.6g / L

[0199] 350nm carboxyl latex 1.0g / L

[0200] Protective agent (sucrose 20g / L, trehalose 20g / L, fructose 20g / L)

[0201] FER rabbit polyclonal antibody 20mg / L (Chengdu Huirui Xinyuan Biotechnology)

[0202] Preservative 0.5ml / L PC300

[0203] Among them, the carboxyl latex latex is subjected to secondary convergent polymerization according to 2017109869707 "A Method for Improving the Antigen Excess and Linear Range of Latex Immunoturbidimetry"; ...

Embodiment 3

[0252] The first reagent R1 contains components at the following concentrations:

[0253] 100mM Hepes-NaOH buffer, pH7.5

[0254] NaCl 2000mM

[0255] Polyethylene glycol 120002g / L

[0256] Tween-2016ml / L

[0257] Deferoxamine 8g / L

[0258] Rabbit polyclonal antibody blocking agent 30ml / L (Fei Peng Bio E-015)

[0259] Preservative 1g / L sodium azide

[0260] The corresponding R2 contains the following concentrations of components:

[0261] 200mM Glycine-Tris buffer pH7.6

[0262] Tween-205ml / L

[0263] 200nm carboxyl latex 2.0g / L

[0264] 350nm carboxyl latex 1.6g / L

[0265] Protective agent (sucrose 10g / L, trehalose 10g / L, fructose 30g / L)

[0266] FER rabbit polyclonal antibody 30mg / L (Chengdu Huirui Xinyuan Biotechnology)

[0267] Preservative 1g / L sodium azide

[0268] Among them, the carboxyl latex latex is subjected to secondary convergent polymerization according to 2017109869707 "A Method for Improving the Antigen Excess and Linear Range of Latex Immunoturbid...

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Abstract

The invention discloses a high-specificity permeability-scattering integrated method ferritin latex turbidimetric detection kit, and belongs to the technical field of biological detection. The invention discloses a high-specificity permeability-scattering integrated method ferritin latex turbidimetric detection kit. The kit comprises a first reagent R1 and a second reagent R2, the R1 is prepared from a Hepes-NaOH buffer solution, NaCl, polyethylene glycol 12000, Tween-20, deferoxamine and a rabbit polyclonal antibody blocking agent; r2 comprises a buffer solution, Tween-20, 200nm carboxyl latex, 350nm carboxyl latex, a protective agent and an FER rabbit polyclonal antibody. According to the invention, the linear range of FER detection range of 1.5-2200ng / ml can be realized; the repeatability CVlt is 2ng / ml; 10%; determining a correlation coefficient gt of a clinical sample test result and a Rogowski ferritin test result; and 0.95%.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a highly specific transmission and scattering integrated method ferritin latex turbidimetric detection kit. Background technique [0002] Ferritin is one of the main forms of iron stored in the body. It has the ability to bind iron and reserve iron to maintain the supply of iron in the body and the relative stability of hemoglobin. The determination of serum ferritin is the most sensitive indicator for checking iron deficiency in the body, and it is used to diagnose iron deficiency anemia, liver disease, etc., and it is also one of the markers of malignant tumors. [0003] Iron deficiency anemia is clinically more common anemia, the main reason is iron deficiency. In recent years, studies have found that ferritin is a specific indicator of iron deficiency anemia, and the occurrence of iron deficiency anemia can be divided into three stages. The first stage is the i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/539G01N33/53G01N21/82
CPCG01N33/6893G01N33/539G01N33/5306G01N21/82G01N2800/22Y02A50/30
Inventor 王泽鹏王钊
Owner BYRON DIAGNOSTICS SHANGHAI
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