Novel coronavirus vaccine candidate strain taking influenza B virus as vector as well as construction method and application of novel coronavirus vaccine candidate strain

A technology of influenza virus, coronavirus, applied in the direction of virus/bacteriophage, application, antiviral agent, etc.

Pending Publication Date: 2022-04-01
ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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  • Abstract
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Problems solved by technology

[0008] The object of the present invention is to provide a new type of coronavirus vaccine candidate strain and its construction method and application using influenza B virus as a carrier, so as to solve the problems existing in the existing new coronavirus vaccine

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  • Novel coronavirus vaccine candidate strain taking influenza B virus as vector as well as construction method and application of novel coronavirus vaccine candidate strain
  • Novel coronavirus vaccine candidate strain taking influenza B virus as vector as well as construction method and application of novel coronavirus vaccine candidate strain
  • Novel coronavirus vaccine candidate strain taking influenza B virus as vector as well as construction method and application of novel coronavirus vaccine candidate strain

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Embodiment Construction

[0056] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0057] 1. Materials and methods

[0058] 1 material

[0059] 1.1 Cells and chick embryos

[0060] MDCK cells and 293T cells were preserved by the Virology Laboratory of Military Veterinary Research Institute, Academy of Military Medical Sciences; 7-9-day-old SPF chicken embryos were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0061] 1.2 Main reagents

[0062] Phusion HF DNAPolymerase was purchased from NEW ENGLAND Biolabs, USA; ReverseTr...

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Abstract

The invention discloses a novel coronavirus vaccine candidate strain taking an influenza B virus as a vector as well as a construction method and application of the novel coronavirus vaccine candidate strain, and relates to the field of novel coronavirus vaccines.After an NS1 gene segment of an influenza B virus attenuated strain B / Yamagata / 16 / 88 is truncated and 110 amino acids are reserved, the NS1 gene segment is inserted into a receptor binding domain gene segment on a genetically synthesized novel coronavirus reference strain S protein, and the novel coronavirus vaccine candidate strain with the influenza B virus as the vector is obtained. The method comprises the following steps: constructing a recombinant plasmid NS110-RBD; a recombinant influenza virus rescued strain rIBV-NS110-RBD which takes an influenza B virus attenuated strain B / Yamagata / 16 / 88 as a skeleton and stably expresses a receptor binding domain (RBD) gene segment on a new coronavirus reference strain S protein is rescued by using a reverse genetics technology. The invention lays a foundation for the research on the packaging mechanism of the influenza B virus and the research and development of the novel coronavirus and novel influenza virus multi-combined vaccine.

Description

technical field [0001] The invention relates to the technical field of novel coronavirus vaccines, in particular to a novel coronavirus vaccine candidate strain with type B influenza virus as a carrier and a construction method and application thereof. Background technique [0002] The novel coronavirus has caused a severe outbreak of COVID-19 worldwide and was declared a pandemic in March 2020. According to the World Health Organization (WHO), as of October 2021, more than 200 countries around the world have reported 235,673,032 confirmed cases, including 4,814,651 deaths. [0003] The new coronavirus (SARS-COV-2) is the main pathogen that causes the new coronavirus pneumonia. It belongs to the Coronaviridae family and the βcoronavirus genus with the other two coronaviruses SARS-CoV and MERS-CoV that infect humans. The new coronavirus is an enveloped, positive, single-stranded RNA virus with a genome of 29903nts, composed of nucleocapsid protein (N), membrane protein (M), ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/86C12N15/50A61K39/215A61P31/14
CPCY02A50/30
Inventor 孙伟洋高玉伟王振飞朱梦涵王铁成冯娜闫飞虎李恩涛李元果赵永坤杨松涛夏咸柱
Owner ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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