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Bone marrow biopsy stationary liquid and preparation method thereof

A technology of fixative and bone marrow, which is applied in the field of medical examination, can solve the problems of prolonging the preparation time of bone marrow tissue specimens, reducing the detection efficiency of bone marrow biopsy results, and destroying tissue morphology and composition, achieving good fixation and decalcification effect, good Fixed effect, effect of promoting decalcification

Pending Publication Date: 2022-04-05
宁波同盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the fixation effect is not good, the structure and composition of the bone marrow tissue will be damaged or changed during the subsequent processing, and if the decalcification effect is not good, the slices will be incomplete or broken, which will affect the pathological diagnosis of the bone marrow tissue.
[0003] At present, the fixation and decalcification of bone marrow tissue are usually carried out in two steps through fixative fixation and decalcification with decalcification solution, which is cumbersome and time-consuming.
Studies have shown that the existing fixative solutions, Bouin's solution and LowyFMA solution, also have dual functions of fixation and decalcification. The effective ingredients for decalcification are glacial acetic acid and picric acid, but the processing time is relatively long, requiring about 96 hours, which is greatly improved. Prolonged the preparation time of bone marrow tissue samples and reduced the detection efficiency of bone marrow biopsy results

Method used

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  • Bone marrow biopsy stationary liquid and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A bone marrow biopsy fixative, comprising the following raw materials: 30 mL of an aqueous formaldehyde solution with a concentration of 37%, 45 mL of an aqueous formic acid solution with a concentration of 20%, 0.25 g of ethylenediaminetetraacetic acid, and a phosphate buffer to make up to 1000 mL, wherein the phosphate buffer The concentration of the liquid was 0.1 mol / L, and the pH was 6.8.

[0021] The preparation method of the above-mentioned bone marrow biopsy fixative comprises the following steps:

[0022] (1), 0.25g of ethylenediaminetetraacetic acid is added in 650mL of phosphate buffer and fully dissolved to obtain the first solution;

[0023] (2), mix the first solution with 30 mL of formaldehyde aqueous solution and 45 mL of formic acid aqueous solution, and then use phosphate buffer to make up the volume to 1000 mL.

[0024] Use the above-mentioned bone marrow biopsy fixative to fix and decalcify the bone marrow biopsy tissue. The specific method is as fo...

Embodiment 2

[0029] A bone marrow biopsy fixative, comprising the following raw materials: 35 mL of an aqueous formaldehyde solution with a concentration of 35%, 50 mL of an aqueous formic acid solution with a concentration of 10%, 0.2 g of ethylenediaminetetraacetic acid, and a phosphate buffer to make up to 1000 mL, wherein the phosphate buffer The concentration of the liquid was 0.1 mol / L, and the pH was 7.2.

[0030] The preparation method of the above-mentioned bone marrow biopsy fixative comprises the following steps:

[0031] (1), 0.2g of ethylenediaminetetraacetic acid is added in 600mL of phosphate buffer and fully dissolved to obtain the first solution;

[0032] (2), mix the first solution with 35 mL of formaldehyde aqueous solution and 50 mL of formic acid aqueous solution, and then make up to 1000 mL with phosphate buffer.

[0033] Use the above-mentioned bone marrow biopsy fixative to fix and decalcify the bone marrow biopsy tissue. The specific method is as follows:

[0034...

Embodiment 3

[0038] A bone marrow biopsy fixative, comprising the following raw materials: 25 mL of a 40%-concentrated formaldehyde solution, 40 mL of a 20%-concentrated formic acid aqueous solution, 0.3 g of ethylenediaminetetraacetic acid, and a phosphate buffer to make up to 1000 mL, wherein the phosphate buffer The concentration of the liquid was 0.1 mol / L, and the pH was 7.0.

[0039] The preparation method of the above-mentioned bone marrow biopsy fixative comprises the following steps:

[0040] (1), 0.3g of ethylenediaminetetraacetic acid is added in 700mL of phosphate buffer and fully dissolved to obtain the first solution;

[0041] (2), mix the first solution with 25 mL of formaldehyde aqueous solution and 40 mL of formic acid aqueous solution, and then use phosphate buffer to make up the volume to 1000 mL to obtain.

[0042] Use the above-mentioned bone marrow biopsy fixative to fix and decalcify the bone marrow biopsy tissue. The specific method is as follows:

[0043] S1. Pla...

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Abstract

The invention discloses a bone marrow biopsy stationary liquid which comprises the following raw materials: 25-35 mL of a formaldehyde aqueous solution, 40-50 mL of a formic acid aqueous solution, 0.2-0.3 g of ethylenediamine tetraacetic acid and the balance of a phosphate buffer solution, wherein the total volume is 1000 mL. The invention also discloses a preparation method of the bone marrow biopsy stationary liquid and a method for carrying out fixed decalcification treatment on bone marrow biopsy tissues by adopting the bone marrow biopsy stationary liquid. The bone marrow biopsy stationary liquid can achieve the purposes of fixation and decalcification at the same time, the content of volatile and irritant substances is low, the quality of a specimen treated by the bone marrow biopsy stationary liquid is good, the treatment time is short, and pathological diagnosis can be rapidly and accurately carried out.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a bone marrow biopsy fixative and a preparation method thereof. Background technique [0002] Bone marrow biopsy is an effective method to obtain bone marrow tissue by using bone marrow aspirate and perform pathological examination. It is usually used for the diagnosis of hematopoietic system diseases, such as the identification and diagnosis of anemia, thrombocytopenia, leukemia and multiple myeloma. . With the development of medicine, bone marrow biopsy has been carried out rapidly and widely used. At present, the main steps of preparing a specimen for bone marrow biopsy include: fixation, decalcification, dehydration, paraffin embedding, sectioning, HE staining, etc. The purpose of decalcification is to remove calcium from hard bone tissue, which is Preparation of tissue sections. Fixation and decalcification are the key links affecting the quality of bone marrow ti...

Claims

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Application Information

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IPC IPC(8): G01N1/30
Inventor 尤丽丽
Owner 宁波同盛生物科技有限公司
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