Haploid breeding device and breeding method for red flowering Chinese cabbage

Abstract

The method comprises the following steps: S1, haploid induction: taking a part of red flowering Chinese cabbage anther to an induction culture medium, performing induction at 19-30 DEG C, checking whether haploid appears or not after induction is performed for 16-32 hours, ending induction when haploid appears, raising the temperature to 25-33 DEG C when haploid does not appear, and taking out the haploid when the haploid does not appear; performing dark culture until haploid embryoid appears; s2, haploid cultivation: inoculating a differentiation culture medium, performing light supplement treatment on the embryos through a light supplement lamp in a cultivation box so as to continuously perform light cultivation, transferring differentiated buds into a rooting culture medium after the differentiated buds grow out, transferring the differentiated buds into the rooting culture medium for cultivation after the differentiated buds appear, and transferring part of the embryos with good growth vigor into a test field for cultivation, and chromosome doubling is carried out through colchicine, so that a good experimental embryo reference is provided before large-scale transfer to a planting field, meanwhile, chromosome doubling is carried out through colchicine, the breeding and planting time is shortened, and the yield is increased.

Description

technical field [0001] The invention relates to the technical field of beetroot breeding, in particular to a haploid breeding device and breeding method for beetroot. Background technique [0002] Cabbage sprouts, also known as seaweed sprouts and red rape sprouts, belong to the same variety as Cantonese cabbage sprouts. They are varieties of Brassica species Brassica subspecies in the family Brassicaceae, a biennial herb. Rich in calcium, phosphorus, iron, carotene, ascorbic acid and other ingredients, multivitamins are higher than Chinese cabbage and pakchoi. The so-called haploid breeding means that haploids are induced by methods such as anther culture and make a single Chromosomes are doubled and paired to become energetic and normal fertile homozygotes, thereby breeding new varieties. [0003] Most of the existing haploid breeding of red cabbage is carried out by heterozygosity, but it is difficult to distinguish heterozygous and homozygous by using heterozygous haplo...

AI Technical Summary

Problems solved by technology

[0003] Most of the existing haploid breeding of red cabbage is carried out by heterozygosity, but it is difficult to distinguish heterozygous and homozygous by using heterozygous haploid breeding, and the gap between heterozygous and homozygous is relatively large. large, and the performance of homozygous is poor in the field, while heterozygous breeding takes a long time, and most of the existing cultivation is carried out in greenhouses and fields, which are not suitable for temperature adjustment that requires small-scale experimental planting. Perfect, the temperature adjustment speed is slow, and the external nutrient delivery can only be replenished manually. Therefore, a haploid breeding device and breeding method for red cabbage sprouts are proposed.

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