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A kind of animal bifidobacterium milk subspecies bla36 for improving constipation and its culture method and application

An animal bifidobacteria, a technology for improving constipation, applied in the field of microbial culture, can solve problems such as limited, achieve obvious advantages, good effect, and improve the effect of constipation reaction

Active Publication Date: 2022-05-27
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the microbial preparations used to improve constipation in the prior art are still very limited, and the effect of these preparations on improving constipation still needs to be further improved

Method used

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  • A kind of animal bifidobacterium milk subspecies bla36 for improving constipation and its culture method and application
  • A kind of animal bifidobacterium milk subspecies bla36 for improving constipation and its culture method and application
  • A kind of animal bifidobacterium milk subspecies bla36 for improving constipation and its culture method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The present embodiment provides a kind of animal Bifidobacterium lactis subsp. lactis BLa36 for improving constipation, and its separation and screening method is as follows:

[0043] Breast milk samples were selected, diluted 10 times with normal saline with a mass concentration of 0.9%, diluted 3 times, and spread on MRS solid medium (the medium contains the antibiotic mupirocin lithium salt, which can inhibit most of the removal of bifidox). Other strains other than Bacillus, the medicines were purchased from Haibo Bio), after culturing at 37 °C for 48 hours, pick out 3 strains of different forms, streak and purify on the surface of MRS solid medium, pick a single colony at 37 °C Use MRS liquid medium to expand the culture, and then use 30% glycerol for preservation. The in vitro physiological characteristics of three preserved strains of human milk-derived single bacteria were tested, and one strain was screened for gastrointestinal fluid tolerance (artificial simul...

Embodiment 2

[0045] The present embodiment carries out morphological identification and 16SrRNA molecular biology identification of Bifidobacterium animalis subsp. lactis BLa36 screened in Example 1, and the steps are as follows:

[0046] (1) Morphological identification:

[0047] The strains were inoculated in MRS solid medium, cultured at 37 °C for 48 h, and observed under a microscope.

[0048] Observation showed that the colonies were milky white, with semi-circular protrusions, smooth and moist surfaces, and neat edges.

[0049] (2) 16S rRNA molecular biology identification:

[0050] The strains stored at -80°C were taken out, inoculated in a centrifuge tube containing 20 mL of MRS liquid medium at a proportion of 2%, cultured at 37°C for 18 h, and then centrifuged, centrifuged at 8000 rpm for 10 min, and the supernatant was removed. liquid to collect bacteria.

[0051] The genome of the strain was extracted, and general bacterial primers were added for PCR amplification, and the a...

Embodiment 3

[0057] The present embodiment optimizes the culture conditions of Bifidobacterium animalis subsp. lactis BLa36, and the steps are as follows:

[0058] Bifidobacterium animalis subsp. lactis BLa36 was inoculated into MRS liquid medium, and cultured at different temperatures of 10-50 °C for 48 h. During the cultivation process, the OD of the culture solution was measured by a microplate reader at intervals. 600 numerical value.

[0059] The results showed that the best growth of Bifidobacterium animalis subsp. lactis BLa36 was at 30-37℃, and the stable growth phase could be reached after 18-24 hours of culture.

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Abstract

The present invention provides a Bifidobacterium subsp. lactis BLa36 that improves constipation and its culture method and application. The Bifidobacterium subsp. lactis BLa36 that improves constipation is named as Bifidobacterium subsp. animalis Bifidobacterium animalis subsp. lactis BLa36 strain, the preservation unit is the General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC No.24029, the preservation date is December 2, 2021, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing . The animal bifidobacterium lactis subspecies has good gastric acid tolerance and can improve constipation.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and relates to a constipation-improving Bifidobacterium animalis subsp. lactis BLa36 and a culture method and application thereof. Background technique [0002] Intractable constipation is a common syndrome. With the changes in social living habits and dietary structure, the incidence of constipation has increased year by year in recent years. Epidemiological surveys show that the incidence of constipation in various countries is 2%-27%. After excluding constipation caused by organic and metabolic diseases, chronic constipation can be divided into 3 subtypes: slow transit constipation (STC), outlet obstruction constipation and mixed constipation, of which STC accounts for the chronic 45.5% of constipation. The concept of STC was first proposed by Proton and Lennar-Joners in 1986. After 30 years of research, it is generally believed that STC is a type of intractable functional cons...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/10A23L33/135C12R1/01
CPCC12R2001/01A61K35/745A61P1/10C12N1/20A23L33/135C12N1/205
Inventor 方曙光董瑶盖忠辉陈珂可朱建国程文
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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