Large-scale production method for plug seedlings of nepenthes in middle Yunnan region
A production method and Nepenthes technology, applied in the field of agriculture, can solve the problems of low initial culture survival rate, browning of explants, and low production efficiency, so as to improve the cultivation effect and efficiency, solve severe browning, and improve efficiency Effect
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Embodiment 1
[0034] (1) Selection and treatment of explants
[0035] On March 16, 2019, select the robust Nepenthes species 'Gaya' plants, spray and disinfect the healthy Nepenthes target plants with 75% (mass ratio) chlorothalonil WP 800 times, pre-selected Terminal buds and lateral buds are used as explants, and the pre-selected terminal buds and lateral buds are covered with shading ziplock bags to avoid light;
[0036] On March 23, 2019, the ziplock bag was opened, and the pretreated terminal buds and lateral buds with a length of 3-5 cm were cut with a sterile scalpel as explant materials, and the leaves were removed; Disinfect the surface of the disinfection dish with 75% (mass ratio) alcohol for 30 seconds; then wash it with sterile water for 3 times, then disinfect it in the mixed disinfectant solution for 8-10 minutes, and then rinse it with sterile water for 3 times; the mixed disinfectant solution For every 100ml of sodium hypochlorite solution with a mass concentration of 2%, ...
Embodiment 2
[0050] Embodiment 2 and embodiment 3 are different from embodiment 1 except that the measures listed in table 1 are the same as embodiment 1, and will not be repeated.
[0051] Table 1: Differences between Embodiment 2, Embodiment 3 and Embodiment 1
[0052]
[0053]
[0054] 2. The influence of different pretreatment of explants and sampling period on the culture effect
[0055] During the research and development of this patented technology, it was found that the explant shading pretreatment and sampling time point have a great influence on the effect of explant culture. After shading treatment, the explant tissues were young and tender, and the usable stem segment had an average of one node more, and the browning was relatively light during the initial and proliferation culture (Table 2). The explants sampled in late March had a high survival rate, fast recovery and less browning, but as the sampling time was delayed, the culture survival rate decreased, and the reco...
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