Large-scale production method for plug seedlings of nepenthes in middle Yunnan region

A production method and Nepenthes technology, applied in the field of agriculture, can solve the problems of low initial culture survival rate, browning of explants, and low production efficiency, so as to improve the cultivation effect and efficiency, solve severe browning, and improve efficiency Effect

Active Publication Date: 2022-04-29
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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Problems solved by technology

[0005] In order to solve technical problems such as low initial culture survival rate, severe browning of explants, high energy consumption, and low production efficiency in the production process of existing Nepenthes tissue culture and production process, the climatic conditions and limited growth cycle in central Yunnan were objectively In practice, a large-scale production method of Nepenthes plug seedlings in central Yunnan is provided through integrated innovation

Method used

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  • Large-scale production method for plug seedlings of nepenthes in middle Yunnan region
  • Large-scale production method for plug seedlings of nepenthes in middle Yunnan region
  • Large-scale production method for plug seedlings of nepenthes in middle Yunnan region

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Experimental program
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Effect test

Embodiment 1

[0034] (1) Selection and treatment of explants

[0035] On March 16, 2019, select the robust Nepenthes species 'Gaya' plants, spray and disinfect the healthy Nepenthes target plants with 75% (mass ratio) chlorothalonil WP 800 times, pre-selected Terminal buds and lateral buds are used as explants, and the pre-selected terminal buds and lateral buds are covered with shading ziplock bags to avoid light;

[0036] On March 23, 2019, the ziplock bag was opened, and the pretreated terminal buds and lateral buds with a length of 3-5 cm were cut with a sterile scalpel as explant materials, and the leaves were removed; Disinfect the surface of the disinfection dish with 75% (mass ratio) alcohol for 30 seconds; then wash it with sterile water for 3 times, then disinfect it in the mixed disinfectant solution for 8-10 minutes, and then rinse it with sterile water for 3 times; the mixed disinfectant solution For every 100ml of sodium hypochlorite solution with a mass concentration of 2%, ...

Embodiment 2

[0050] Embodiment 2 and embodiment 3 are different from embodiment 1 except that the measures listed in table 1 are the same as embodiment 1, and will not be repeated.

[0051] Table 1: Differences between Embodiment 2, Embodiment 3 and Embodiment 1

[0052]

[0053]

[0054] 2. The influence of different pretreatment of explants and sampling period on the culture effect

[0055] During the research and development of this patented technology, it was found that the explant shading pretreatment and sampling time point have a great influence on the effect of explant culture. After shading treatment, the explant tissues were young and tender, and the usable stem segment had an average of one node more, and the browning was relatively light during the initial and proliferation culture (Table 2). The explants sampled in late March had a high survival rate, fast recovery and less browning, but as the sampling time was delayed, the culture survival rate decreased, and the reco...

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Abstract

A large-scale production method for plug seedlings of nepenthes in the middle Yunnan region is characterized in that more than 50 high-quality plug seedlings are bred from one terminal bud explant in March to October in the same year through compact connection and technical measure integration of seven steps of explant pretreatment, initiation, proliferation, subculture, seedling strengthening and rooting culture, tissue culture seedling transplanting and field management; through explant pretreatment, an improved WPM culture medium with low salt content is selected, so that the problems of low survival rate of initial culture, serious browning and slow growth of explants in subsequent group culture links are solved; an LED combined light source I and an LED combined light source II are adopted in the multiplication culture link and the strong seedling culture link respectively, compared with a traditional fluorescent lamp, energy is saved by 43.7%-53%, and meanwhile the multiplication multiple and the strong seedling rate are improved by 38.1% and 28.0% respectively; in the links of rooting culture, tissue culture seedling transplanting and field management, the beneficial weather conditions of relatively damp and hot summer in the middle Yunnan region are fully utilized, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and in particular relates to a method for large-scale production of Nepenthes plug seedlings in central Yunnan. Background technique [0002] Nepenthes, also known as piglet cages, thunder pots, etc., are perennial climbing woody vines of the family Nepenthaceae (Nepenthaceae), native to southeastern Asia and northern Oceania, and there are about 100 species in the world. The remaining species are distributed in some areas such as Zhanjiang, Zhuhai, and Zhongshan in Hainan and Guangdong in my country, but the native species are rarely used. It was not until the 1990s that the excellent varieties of Nepenthes obtained by interspecific hybridization abroad were introduced and gradually used in production applications. [0003] Nepenthes has become an eye-catching ornamental plant, and the cage-shaped metamorphosis leaves derived from leaves have a unique shape and insectivorous characteristic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002Y02P60/14
Inventor 阮继伟余蓉培瞿素萍吴丽芳杨春梅单芹丽吴旻卢珍红
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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