Method for extracting purified royal jelly major protein from royal jelly

Abstract

The invention discloses a method for extracting purified royal jelly major protein from royal jelly. Comprising the following steps: S1, preparing a buffer solution to carry out oscillation treatment on royal jelly; s2, filtering the solution I; s3, carrying out centrifugal treatment on the solution II; s4, performing protein precipitation on the centrifugal supernatant; s5, carrying out purification treatment on the obtained royal jelly major protein; the royal jelly is moderated by setting the buffer solution, the activity of main protein in the royal jelly is maintained, inactivation is not caused, and impurities in the royal jelly are effectively filtered by the ultrafiltration membrane with the molecular weight cut-off of 100kDa, so that the royal jelly quality is improved. The method comprises the following steps: firstly, extracting the royal jelly main protein, then, carrying out effective crude extraction on the royal jelly main protein through centrifugal equipment and an ultrafiltration membrane with the molecular weight cut-off of 49kDa, keeping few impurities in the royal jelly main protein, and then, purifying the royal jelly main protein through ion exchange chromatography, so that the purity of the royal jelly main protein is relatively high.

Description

technical field [0001] The invention belongs to the technical field of protein extraction and purification, and in particular relates to a method for extracting and purifying royal jelly main protein from royal jelly. Background technique [0002] Royal jelly, also known as royal jelly, royal jelly, royal jelly, bee milk, is the secretion of the pharyngeal glands of young workers who cultivate larvae in the honeycomb. It is the food for the larvae that will become the queen bee and the food for the queen bee for life. , the protein components of royal jelly are mainly composed of royal jelly major proteins 1, 2 and 3, each of which has unique physiological functions and is beneficial to human health. However, the traditional purification methods of main royal jelly proteins 1, 2, and 3 mainly include dialysis, superposition and combination of multiple chromatographic separation methods, etc. However, various extraction and purification methods on the market still have variou...

AI Technical Summary

Problems solved by technology

However, the traditional purification methods of main royal jelly protein 1, 2, and 3 mainly include dialysis, superposition and combination of multiple chromatographic separation methods, etc. However, various extraction and purification methods on the market still have various problems

[0003] Such as authorized announcement number is the ultrafiltration membrane separation method that obtains royal jelly main protein and active filtrate liquid in the disclosed royal jelly of CN103772497A, although it has realized the extraction rate of MRJPs reaches 82.63%, becomes the frozen protein content greater than 70% through freeze-drying Dry powder; the filtrate is used to prepare beverages to realize the comprehensive utilization of royal jelly, but it does not solve the existing problems in the extraction and purification of the main protein of royal jelly, which cannot maintain the activity of the protein, and cannot realize the rough and fine purification of the main protein , improving the purity of main protein, etc., for this reason we propose a method for extracting and purifying royal jelly main protein from royal jelly

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